AB137025

Anti-Ubiquitin antibody [EPR8590]

20ul selling size
RabMAb
Recombinant
What is this?

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(9 Publications)

Rabbit Recombinant Monoclonal UBC antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 9 publications.

View Alternative Names

Polyubiquitin-C, UBC

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin antibody [EPR8590] (AB137025)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin antibody [EPR8590] (AB137025)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling Ubiquitin with ab137025 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Lab

Western blot - Anti-Ubiquitin antibody [EPR8590] (AB137025)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. ab137025 and ab8245 (loading control to GAPDH) were diluted 1/200 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-Ubiquitin antibody [EPR8590] (ab137025) at 1/200 dilution

Lane 1:

MCF-7 cell lysate at 20 µg

Lane 2:

MCF-7 Whole cell lysate + M132 (50 uM 90 min) at 20 µg

Lane 3:

Mouse brain tissue lysate at 20 µg

Predicted band size: 26 kDa

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Unknown

Western blot - Anti-Ubiquitin antibody [EPR8590] (AB137025)

All lanes:

Western blot - Anti-Ubiquitin antibody [EPR8590] (ab137025) at 1/1000 dilution

Lane 1:

Fetal brain cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 26 kDa

false

Carrier free

Anti-Ubiquitin antibody [EPR8590] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8590

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab137025 recognizes Ubiquitin, polyubiquitin and ubiquitinated proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ubiquitin is a small regulatory protein found in almost all tissues of eukaryotic organisms. It has a molecular weight of approximately 8.6 kilodaltons. It functions mechanically by attaching to proteins through a process called ubiquitination which involves the formation of an isopeptide bond. Ubiquitin molecules can form polyubiquitin chains through different lysine residues such as K48 and K63 that determine their function. These chains label substrate proteins for various fates including degradation. Ubiquitin is expressed ubiquitously in cells reflecting its essential role in maintaining protein homeostasis.

Biological function summary

The ubiquitin system plays a critical role in regulating protein turnover and quality control within cells. It is part of a larger complex known as the ubiquitin-proteasome system (UPS) which is responsible for degrading proteins that need to be turned over. This process is essential for cell cycle control response to oxidative stress and DNA repair. Ubiquitin's role in tagging proteins for degradation or signaling allows cells to respond quickly to changes in their environment and maintain balance.

Pathways

Ubiquitin functions in several important biological pathways including the Wnt and NF-kB pathways. In the Wnt signaling pathway ubiquitination modulates the stability of key components thereby affecting the pathway's overall activity. In the NF-kB signaling pathway ubiquitin labels inhibitor proteins for degradation which releases and activates NF-kB. These pathways highlight ubiquitin's interaction with proteins such as beta-catenin in Wnt and IkB in NF-kB illustrating how it regulates diverse cellular processes.

The dysfunction of the ubiquitin system is linked to neurodegenerative diseases and cancers. Ubiquitin-related defects in protein degradation can lead to the buildup of unwanted proteins contributing to conditions like Parkinson's disease. Connections with cancer are evident as ubiquitin controls cell cycle proteins and aberrant ubiquitination may drive tumor growth and progression. The protein p53 known to be controlled by ubiquitination plays a significant role in cancer related mechanisms when dysregulated. Understanding and targeting ubiquitin-related pathways may provide new therapeutic opportunities for treating these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ubiquitin. Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked : Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in proteotoxic stress response and cell cycle; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. During ubiquitination, the acceptor ubiquitin is positioned in the active site via direct interaction with the E2 ubiquitin-conjugating enzymes such as UBE2R2 (PubMed : 38326650). As a monoubiquitin, its C-terminal glycine is recognized as a C-degron by Cul2-RING (CRL2) E3 ubiquitin-protein ligase complexes (PubMed : 39548056).

See full target information UBC

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Clinical and experimental pharmacology & physiology 51:e13855 PubMed38636942

2024

Salvianic acid A sodium facilitates cardiac microvascular endothelial cell proliferation by enhancing the hypoxia-inducible factor-1 alpha/vascular endothelial growth factor signalling pathway post-myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Jichun Liu,Fei Wu,Zhenhan Li,Shengwei Zheng,Yanqiang Huang,Hao Chen

Cell reports 39:110656 PubMed35417709

2022

An oxygen-adaptive interaction between SNHG12 and occludin maintains blood-brain barrier integrity.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Li,Jia-Yi Wei,Hui Liu,Kang-Ji Wang,Sheng-Nan Jin,Zheng-Kang Su,Hui-Jie Wang,Jun-Xiu Shi,Bo Li,De-Shu Shang,Wen-Gang Fang,Xiao-Xue Qin,Wei-Dong Zhao,Yu-Hua Chen

Cancer science 113:1652-1668 PubMed35293097

2022

Exosomal miR-106a-5p accelerates the progression of nasopharyngeal carcinoma through FBXW7-mediated TRIM24 degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Chang-Wu Li,Jing Zheng,Guo-Qing Deng,Yu-Guang Zhang,Yue Du,Hong-Yan Jiang

Cell death & disease 11:297 PubMed32341332

2020

Chidamide epigenetically represses autophagy and exerts cooperative antimyeloma activity with bortezomib.

Applications

Unspecified application

Species

Unspecified reactive species

Li Xu,Juan Feng,Hailong Tang,Ying Dong,Mimi Shu,Xiequn Chen

Development (Cambridge, England) 147: PubMed31862842

2020

Deficient autophagy in epithelial stem cells drives aging in the freshwater cnidarian .

Applications

Unspecified application

Species

Unspecified reactive species

Szymon Tomczyk,Nenad Suknovic,Quentin Schenkelaars,Yvan Wenger,Kazadi Ekundayo,Wanda Buzgariu,Christoph Bauer,Kathleen Fischer,Steven Austad,Brigitte Galliot

EBioMedicine 49:26-39 PubMed31636012

2019

The LIV-1-GRPEL1 axis adjusts cell fate during anti-mitotic agent-damaged mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Pingbo Chen,Beibei Wang,Qingqing Mo,Peng Wu,Yong Fang,Yuan Tian,Xin Jin,Yue Gao,Yuan Wu,Yang Cao,Yang Zhang,Ling Xi,Shixuan Wang,Junbo Hu,Ding Ma,Jianfeng Zhou,Qinglei Gao,Gang Chen

Journal of molecular and cellular cardiology 129:303-313 PubMed30885746

2019

In vitro analysis of arrhythmogenic cardiomyopathy associated desmoglein-2 (DSG2) mutations reveals diverse glycosylation patterns.

Applications

Unspecified application

Species

Unspecified reactive species

Jana Davina Debus,Hendrik Milting,Andreas Brodehl,Astrid Kassner,Dario Anselmetti,Jan Gummert,Anna Gaertner-Rommel

Journal of cell science 131: PubMed29748379

2018

SOD1 aggregation alters ubiquitin homeostasis in a cell model of ALS.

Applications

Unspecified application

Species

Unspecified reactive species

Natalie E Farrawell,Isabella Lambert-Smith,Kristen Mitchell,Jessie McKenna,Luke McAlary,Prajwal Ciryam,Kara L Vine,Darren N Saunders,Justin J Yerbury

PloS one 8:e74051 PubMed24040163

2013

Hypoxia-inducible factor-1alpha and MAPK co-regulate activation of hepatic stellate cells upon hypoxia stimulation.

Applications

Species

Unspecified reactive species

Yueqin Wang,Yimin Huang,Fei Guan,Yan Xiao,Jing Deng,Huoying Chen,Xiaolin Chen,Jianrong Li,Hanju Huang,Chunwei Shi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ubiquitin antibody [EPR8590]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin antibody [EPR8590] (AB137025)

Western blot - Anti-Ubiquitin antibody [EPR8590] (AB137025)

Western blot - Anti-Ubiquitin antibody [EPR8590] (AB137025)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Product promise