Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034]
- 20ul selling size
- RabMAb
- Recombinant
- What is this?
4
(1 Review)
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(24 Publications)
Rabbit Recombinant Monoclonal UBC Linkage-specific K27 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Rat, Mouse, Human samples. Cited in 24 publications.
View Alternative Names
Polyubiquitin-C, UBC
- Flow Cyt (Intra)
Supplier Data
Flow Cytometry (Intracellular) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (AB181537)
Intracellular flow cytometric analysis of2% paraformaldehyde-fixedJurkat (Human T cell leukemia cells from peripheral blood) cells labeling Ubiquitin (linkage-specific K27) with ab181537 at 1/160 dilution (red). The secondary antibody wasGoat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control isRabbit monoclonal IgG (black) and cell without incubation with primary antibody and secondary antibody is blue.
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (AB181537)
Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma)
labelling Ubiquitin (linkage-specific K27) with purified ab181537 at 1/1000. Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (AB181537)
Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder labeling Ubiquitin (linkage-specific K27) with ab181537 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on cancer cells of Human bladder transitional cell carcinoma is observed. Counter stained with Hematoxylin.
Negative control : Used PBS instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (AB181537)
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Ubiquitin (linkage-specific K27) with ab181537 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on lymphocytes of Rat spleen is observed. Counter stained with Hematoxylin.
Negative control : Used PBS instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (AB181537)
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Ubiquitin (linkage-specific K27) with ab181537 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on lymphocytes of Mouse spleen is observed. Counter stained with Hematoxylin.
Negative control : Used PBS instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- WB
Supplier Data
Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (AB181537)
Blocking/dilution buffer : 5% BSA/TBST.
All lanes:
Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537) at 1/5000 dilution
All lanes:
HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates at 10 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDa
false
- WB
Supplier Data
Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (AB181537)
Blocking/Dilution buffer : 5% BSA/TBST.
All lanes:
Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537) at 1/50000 dilution
Lane 1:
293T (Human epithelial cells from embryonic kidney) whole cell lysates at 10 µg
Lane 2:
Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates at 10 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDa
false
- WB
Supplier Data
Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (AB181537)
Blocking/dilution buffer : 5% BSA/TBST.
All lanes:
Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537) at 1/5000 dilution
Lane 1:
Mouse brain lysates at 10 µg
Lane 2:
Mouse heart lysates at 10 µg
Lane 3:
Mouse kidney lysates at 10 µg
Lane 4:
Mouse spleen lysates at 10 µg
Lane 5:
Rat brain lysates at 10 µg
Lane 6:
Rat heart lysates at 10 µg
Lane 7:
Rat kidney lysates at 10 µg
Lane 8:
Rat spleen lysates at 10 µg
Lane 9:
C6 (Rat glial tumor cells) whole cell lysates at 10 µg
Lane 10:
RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates at 10 µg
Lane 11:
PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 10 µg
Lane 12:
NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates at 10 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDa,14 kDa
false
- WB
Supplier Data
Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (AB181537)
Blocking/dilution buffer : 5% BSA/TBST.
Expected MW is ~17kDa (8.5*2)
The ~30kDa band may be the dimer of K27-linked-Ub2 recombinant protein.
All lanes:
Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537) at 1/5000 dilution
Lane 1:
K27-linked-Ub2 recombinant protein at 0.02 µg
Lane 2:
K6-linked-Ub2 recombinant protein at 0.02 µg
Lane 3:
K11-linked-Ub2 recombinant protein at 0.02 µg
Lane 4:
K29-linked-Ub2 recombinant protein at 0.02 µg
Lane 5:
K33-linked-Ub2 recombinant protein at 0.02 µg
Lane 6:
K48-linked-Ub2 recombinant protein at 0.02 µg
Lane 7:
K63-linked-Ub2 recombinant protein at 0.02 µg
Lane 8:
Ubiquitin recombinant protein at 0.02 µg
Lane 9:
Ubiquitin linear recombinant protein at 0.02 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 26 kDa
false
Related conjugates and formulations (9)
-
HRP Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034]
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519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034]
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665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034]
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Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] - BSA and Azide free
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565 Alexa Fluor® 555
Alexa Fluor® 555 Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034]
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617 Alexa Fluor® 594
Alexa Fluor® 594 Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034]
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578 PE
PE Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034]
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660 APC
APC Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034]
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775 Alexa Fluor® 750
Alexa Fluor® 750 Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034]
Reactivity data
Product details
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The ubiquitin system plays a critical role in regulating protein turnover and quality control within cells. It is part of a larger complex known as the ubiquitin-proteasome system (UPS) which is responsible for degrading proteins that need to be turned over. This process is essential for cell cycle control response to oxidative stress and DNA repair. Ubiquitin's role in tagging proteins for degradation or signaling allows cells to respond quickly to changes in their environment and maintain balance.
Pathways
Ubiquitin functions in several important biological pathways including the Wnt and NF-kB pathways. In the Wnt signaling pathway ubiquitination modulates the stability of key components thereby affecting the pathway's overall activity. In the NF-kB signaling pathway ubiquitin labels inhibitor proteins for degradation which releases and activates NF-kB. These pathways highlight ubiquitin's interaction with proteins such as beta-catenin in Wnt and IkB in NF-kB illustrating how it regulates diverse cellular processes.
Product protocols
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Target data
Publications (24)
Recent publications for all applications. Explore the full list and refine your search
Nature communications 16:7360 PubMed40783390
2025
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PLoS pathogens 21:e1013333 PubMed40623121
2025
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Journal of biomedical science 32:26 PubMed39972304
2025
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Nature communications 15:8519 PubMed39353976
2024
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Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2400666 PubMed39136283
2024
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Cell reports. Medicine 5:101510 PubMed38614093
2024
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Molecular cancer research : MCR 21:578-590 PubMed36940483
2023
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Nature communications 13:5493 PubMed36123338
2022
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Cellular & molecular immunology 19:1117-1129 PubMed35982175
2022
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Signal transduction and targeted therapy 7:264 PubMed35918322
2022
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com