JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB181537

Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034]

4

(1 Review)

|

(24 Publications)

Rabbit Recombinant Monoclonal UBC Linkage-specific K27 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Rat, Mouse, Human samples. Cited in 24 publications.

View Alternative Names

Polyubiquitin-C, UBC

9 Images
Flow Cytometry (Intracellular) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (AB181537)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (AB181537)

Intracellular flow cytometric analysis of2% paraformaldehyde-fixedJurkat (Human T cell leukemia cells from peripheral blood) cells labeling Ubiquitin (linkage-specific K27) with ab181537 at 1/160 dilution (red). The secondary antibody wasGoat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control isRabbit monoclonal IgG (black) and cell without incubation with primary antibody and secondary antibody is blue.

Immunocytochemistry/ Immunofluorescence - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (AB181537)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (AB181537)

Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma)
labelling Ubiquitin (linkage-specific K27) with purified ab181537 at 1/1000. Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (AB181537)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (AB181537)

Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder labeling Ubiquitin (linkage-specific K27) with ab181537 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on cancer cells of Human bladder transitional cell carcinoma is observed. Counter stained with Hematoxylin.
Negative control : Used PBS instead of primary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (AB181537)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (AB181537)

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Ubiquitin (linkage-specific K27) with ab181537 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on lymphocytes of Rat spleen is observed. Counter stained with Hematoxylin.
Negative control : Used PBS instead of primary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (AB181537)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (AB181537)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Ubiquitin (linkage-specific K27) with ab181537 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on lymphocytes of Mouse spleen is observed. Counter stained with Hematoxylin.
Negative control : Used PBS instead of primary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (AB181537)
  • WB

Supplier Data

Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (AB181537)

Blocking/dilution buffer : 5% BSA/TBST.

All lanes:

Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537) at 1/5000 dilution

All lanes:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 26 kDa

Observed band size: 26 kDa

false

Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (AB181537)
  • WB

Supplier Data

Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (AB181537)

Blocking/Dilution buffer : 5% BSA/TBST.

All lanes:

Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537) at 1/50000 dilution

Lane 1:

293T (Human epithelial cells from embryonic kidney) whole cell lysates at 10 µg

Lane 2:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 26 kDa

Observed band size: 26 kDa

false

Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (AB181537)
  • WB

Supplier Data

Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (AB181537)

Blocking/dilution buffer : 5% BSA/TBST.

All lanes:

Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537) at 1/5000 dilution

Lane 1:

Mouse brain lysates at 10 µg

Lane 2:

Mouse heart lysates at 10 µg

Lane 3:

Mouse kidney lysates at 10 µg

Lane 4:

Mouse spleen lysates at 10 µg

Lane 5:

Rat brain lysates at 10 µg

Lane 6:

Rat heart lysates at 10 µg

Lane 7:

Rat kidney lysates at 10 µg

Lane 8:

Rat spleen lysates at 10 µg

Lane 9:

C6 (Rat glial tumor cells) whole cell lysates at 10 µg

Lane 10:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates at 10 µg

Lane 11:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 10 µg

Lane 12:

NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 26 kDa

Observed band size: 26 kDa,14 kDa

false

Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (AB181537)
  • WB

Supplier Data

Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (AB181537)

Blocking/dilution buffer : 5% BSA/TBST.

Expected MW is ~17kDa (8.5*2)

The ~30kDa band may be the dimer of K27-linked-Ub2 recombinant protein.

All lanes:

Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537) at 1/5000 dilution

Lane 1:

K27-linked-Ub2 recombinant protein at 0.02 µg

Lane 2:

K6-linked-Ub2 recombinant protein at 0.02 µg

Lane 3:

K11-linked-Ub2 recombinant protein at 0.02 µg

Lane 4:

K29-linked-Ub2 recombinant protein at 0.02 µg

Lane 5:

K33-linked-Ub2 recombinant protein at 0.02 µg

Lane 6:

K48-linked-Ub2 recombinant protein at 0.02 µg

Lane 7:

K63-linked-Ub2 recombinant protein at 0.02 µg

Lane 8:

Ubiquitin recombinant protein at 0.02 µg

Lane 9:

Ubiquitin linear recombinant protein at 0.02 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 26 kDa

false

  • HRP

    HRP Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034]

  • Carrier free

    Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] - BSA and Azide free

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034]

  • 578 PE

    PE Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034]

  • 660 APC

    APC Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17034

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p>Actual Band: 14kDa and above.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/160", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p>Actual Band: 14kDa and above.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p>Actual Band: 14kDa and above.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>" } } }

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ubiquitin is a small regulatory protein found in almost all tissues of eukaryotic organisms. It has a molecular weight of approximately 8.6 kilodaltons. It functions mechanically by attaching to proteins through a process called ubiquitination which involves the formation of an isopeptide bond. Ubiquitin molecules can form polyubiquitin chains through different lysine residues such as K48 and K63 that determine their function. These chains label substrate proteins for various fates including degradation. Ubiquitin is expressed ubiquitously in cells reflecting its essential role in maintaining protein homeostasis.
Biological function summary

The ubiquitin system plays a critical role in regulating protein turnover and quality control within cells. It is part of a larger complex known as the ubiquitin-proteasome system (UPS) which is responsible for degrading proteins that need to be turned over. This process is essential for cell cycle control response to oxidative stress and DNA repair. Ubiquitin's role in tagging proteins for degradation or signaling allows cells to respond quickly to changes in their environment and maintain balance.

Pathways

Ubiquitin functions in several important biological pathways including the Wnt and NF-kB pathways. In the Wnt signaling pathway ubiquitination modulates the stability of key components thereby affecting the pathway's overall activity. In the NF-kB signaling pathway ubiquitin labels inhibitor proteins for degradation which releases and activates NF-kB. These pathways highlight ubiquitin's interaction with proteins such as beta-catenin in Wnt and IkB in NF-kB illustrating how it regulates diverse cellular processes.

The dysfunction of the ubiquitin system is linked to neurodegenerative diseases and cancers. Ubiquitin-related defects in protein degradation can lead to the buildup of unwanted proteins contributing to conditions like Parkinson's disease. Connections with cancer are evident as ubiquitin controls cell cycle proteins and aberrant ubiquitination may drive tumor growth and progression. The protein p53 known to be controlled by ubiquitination plays a significant role in cancer related mechanisms when dysregulated. Understanding and targeting ubiquitin-related pathways may provide new therapeutic opportunities for treating these conditions.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Ubiquitin. Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked : Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in proteotoxic stress response and cell cycle; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. During ubiquitination, the acceptor ubiquitin is positioned in the active site via direct interaction with the E2 ubiquitin-conjugating enzymes such as UBE2R2 (PubMed : 38326650). As a monoubiquitin, its C-terminal glycine is recognized as a C-degron by Cul2-RING (CRL2) E3 ubiquitin-protein ligase complexes (PubMed : 39548056).
See full target information UBC Linkage-specific K27

Publications (24)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:7360 PubMed40783390

2025

Opposing regulation of the K63-linked polyubiquitination of RIPK3 by SMURF1 and USP5 in necroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Chi Hyun Hwang,Minhong Lee,Ju Won Kim,Young Woo Nam,Gyuho Hwang,Hyun Sung Ryu,Jinho Seo,Eun-Woo Lee,Hyuk Wan Ko,Jaewhan Song

PLoS pathogens 21:e1013333 PubMed40623121

2025

RNF213 regulates blood‒brain barrier integrity by targeting TRAF3 for type I interferon activation during A. baumannii infection.

Applications

Unspecified application

Species

Unspecified reactive species

Yanfeng Li,Qingqing Xie,Luyu Yang,Qian Jiang,Zhiping Liu,Chengjiang Gao,Xiaopeng Qi,Tao Xu

Journal of biomedical science 32:26 PubMed39972304

2025

K27-linked RORγt ubiquitination by Nedd4 potentiates Th17-mediated autoimmunity.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuming Zeng,Hui Guo,Na Tang,Pranav S Renavikar,Nitin J Karandikar,Amy E Lovett-Racke,Michael K Racke,Chengkai Yan,Rong Tang,Sushmita Sinha,Krishnendu Ghosh,Jeremy P Ryal,Song Ouyang,Min Chen,Foued Amari,Coppola Vincenzo,R Marshall Pope,Yalan Li,Huan Yang,Wallace Y Langdon,Jian Zhang

Nature communications 15:8519 PubMed39353976

2024

CHIP ameliorates nonalcoholic fatty liver disease via promoting K63- and K27-linked STX17 ubiquitination to facilitate autophagosome-lysosome fusion.

Applications

Unspecified application

Species

Unspecified reactive species

Hyunjin Rho,Seungyeon Kim,Seung Up Kim,Jeong Won Kim,Sang Hoon Lee,Sang Hoon Park,Freddy E Escorcia,Joon-Yong Chung,Jaewhan Song

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2400666 PubMed39136283

2024

CDK4/6 Inhibitors Impede Chemoresistance and Inhibit Tumor Growth of Small Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Wen,Xue Sun,Lingge Zeng,Shumei Liang,Deyu Li,Xiangtian Chen,Fanrui Zeng,Chao Zhang,Qiongyao Wang,Qinsong Zhong,Ling Deng,Linlang Guo

Cell reports. Medicine 5:101510 PubMed38614093

2024

Arachidonic acid released by PIK3CA mutant tumor cells triggers malignant transformation of colonic epithelium by inducing chromatin remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Baoyu He,Qingli Bie,Rou Zhao,Yugang Yan,Guanjun Dong,Baogui Zhang,Sen Wang,Wenrong Xu,Dongxing Tian,Yujun Hao,Yanhua Zhang,Mingsheng Zhao,Huabao Xiong,Bin Zhang

Molecular cancer research : MCR 21:578-590 PubMed36940483

2023

USP10 Regulates ZEB1 Ubiquitination and Protein Stability to Inhibit ZEB1-Mediated Colorectal Cancer Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Sun,Jia Yu,Justin Guinney,Bo Qin,Frank A Sinicrope

Nature communications 13:5493 PubMed36123338

2022

Extracellular fibrinogen-binding protein released by intracellular Staphylococcus aureus suppresses host immunity by targeting TRAF3.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaokai Zhang,Tingrong Xiong,Lin Gao,Yu Wang,Luxuan Liu,Tian Tian,Yun Shi,Jinyong Zhang,Zhuo Zhao,Dongshui Lu,Ping Luo,Weijun Zhang,Ping Cheng,Haiming Jing,Qiang Gou,Hao Zeng,Dapeng Yan,Quanming Zou

Cellular & molecular immunology 19:1117-1129 PubMed35982175

2022

The membrane-associated ubiquitin ligases MARCH2 and MARCH3 target IL-5 receptor alpha to negatively regulate eosinophilic airway inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Lin-Wen Zeng,Lu Feng,Rui Liu,Heng Lin,Hong-Bing Shu,Shu Li

Signal transduction and targeted therapy 7:264 PubMed35918322

2022

Targeting HECTD3-IKKα axis inhibits inflammation-related metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Fubing Li,Huichun Liang,Hua You,Ji Xiao,Houjun Xia,Xi Chen,Maobo Huang,Zhuo Cheng,Chuanyu Yang,Wenjing Liu,Hailin Zhang,Li Zeng,Yingying Wu,Fei Ge,Zhen Li,Wenhui Zhou,Yi Wen,Zhongmei Zhou,Rong Liu,Dewei Jiang,Ni Xie,Bin Liang,Zhenzhen Liu,Yanjie Kong,Ceshi Chen
View all publications

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com