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Rabbit Recombinant Monoclonal antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Rat, Mouse, Human samples. Cited in 16 publications.


Images

Immunocytochemistry/ Immunofluorescence - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (AB181537), expandable thumbnail
  • Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (AB181537), expandable thumbnail
  • Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (AB181537), expandable thumbnail
  • Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (AB181537), expandable thumbnail
  • Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (AB181537), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Mouse
Tested
Expected
Expected
Tested
Rat
Tested
Expected
Expected
Tested

Tested
Tested

Species
Rat
Dilution info
1/5000
Notes

Actual Band: 14kDa and above.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
1/5000
Notes

Actual Band: 14kDa and above.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/5000
Notes

Actual Band: 14kDa and above.

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/160
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

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Rabbit Recombinant Monoclonal antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Rat, Mouse, Human samples. Cited in 16 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR17034
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Ubiquitin is a small regulatory protein found in almost all tissues of eukaryotic organisms. It has a molecular weight of approximately 8.6 kilodaltons. It functions mechanically by attaching to proteins through a process called ubiquitination which involves the formation of an isopeptide bond. Ubiquitin molecules can form polyubiquitin chains through different lysine residues such as K48 and K63 that determine their function. These chains label substrate proteins for various fates including degradation. Ubiquitin is expressed ubiquitously in cells reflecting its essential role in maintaining protein homeostasis.

Biological function summary

The ubiquitin system plays a critical role in regulating protein turnover and quality control within cells. It is part of a larger complex known as the ubiquitin-proteasome system (UPS) which is responsible for degrading proteins that need to be turned over. This process is essential for cell cycle control response to oxidative stress and DNA repair. Ubiquitin's role in tagging proteins for degradation or signaling allows cells to respond quickly to changes in their environment and maintain balance.

Pathways

Ubiquitin functions in several important biological pathways including the Wnt and NF-kB pathways. In the Wnt signaling pathway ubiquitination modulates the stability of key components thereby affecting the pathway's overall activity. In the NF-kB signaling pathway ubiquitin labels inhibitor proteins for degradation which releases and activates NF-kB. These pathways highlight ubiquitin's interaction with proteins such as beta-catenin in Wnt and IkB in NF-kB illustrating how it regulates diverse cellular processes.

Associated diseases and disorders

The dysfunction of the ubiquitin system is linked to neurodegenerative diseases and cancers. Ubiquitin-related defects in protein degradation can lead to the buildup of unwanted proteins contributing to conditions like Parkinson's disease. Connections with cancer are evident as ubiquitin controls cell cycle proteins and aberrant ubiquitination may drive tumor growth and progression. The protein p53 known to be controlled by ubiquitination plays a significant role in cancer related mechanisms when dysregulated. Understanding and targeting ubiquitin-related pathways may provide new therapeutic opportunities for treating these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma)
    labelling Ubiquitin (linkage-specific K27) with purified ab181537 at 1/1000. Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

  • Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537), expandable thumbnail

    Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537)

    Blocking/dilution buffer: 5% BSA/TBST.

    Expected MW is ~17kDa (8.5*2)

    The ~30kDa band may be the dimer of K27-linked-Ub2 recombinant protein.

    All lanes: Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537) at 1/5000 dilution

    Lane 1: K27-linked-Ub2 recombinant protein at 0.02 µg

    Lane 2: K6-linked-Ub2 recombinant protein at 0.02 µg

    Lane 3: K11-linked-Ub2 recombinant protein at 0.02 µg

    Lane 4: K29-linked-Ub2 recombinant protein at 0.02 µg

    Lane 5: K33-linked-Ub2 recombinant protein at 0.02 µg

    Lane 6: K48-linked-Ub2 recombinant protein at 0.02 µg

    Lane 7: K63-linked-Ub2 recombinant protein at 0.02 µg

    Lane 8: Ubiquitin recombinant protein at 0.02 µg

    Lane 9: Ubiquitin linear recombinant protein at 0.02 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 26 kDa

  • Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537), expandable thumbnail

    Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537)

    Blocking/Dilution buffer: 5% BSA/TBST.

    All lanes: Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537) at 1/50000 dilution

    Lane 1: 293T (Human epithelial cells from embryonic kidney) whole cell lysates at 10 µg

    Lane 2: Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 26 kDa

    Observed band size: 26 kDa

  • Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537), expandable thumbnail

    Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537)

    Blocking/dilution buffer: 5% BSA/TBST.

    All lanes: Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537) at 1/5000 dilution

    All lanes: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 26 kDa

    Observed band size: 26 kDa

  • Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537), expandable thumbnail

    Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537)

    Blocking/dilution buffer: 5% BSA/TBST.

    All lanes: Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537) at 1/5000 dilution

    Lane 1: Mouse brain lysates at 10 µg

    Lane 2: Mouse heart lysates at 10 µg

    Lane 3: Mouse kidney lysates at 10 µg

    Lane 4: Mouse spleen lysates at 10 µg

    Lane 5: Rat brain lysates at 10 µg

    Lane 6: Rat heart lysates at 10 µg

    Lane 7: Rat kidney lysates at 10 µg

    Lane 8: Rat spleen lysates at 10 µg

    Lane 9: C6 (Rat glial tumor cells) whole cell lysates at 10 µg

    Lane 10: RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates at 10 µg

    Lane 11: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 10 µg

    Lane 12: NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 26 kDa

    Observed band size: 26 kDa, 14 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537)

    Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder labeling Ubiquitin (linkage-specific K27) with ab181537 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear staining on cancer cells of Human bladder transitional cell carcinoma is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Ubiquitin (linkage-specific K27) with ab181537 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear staining on lymphocytes of Mouse spleen is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537)

    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Ubiquitin (linkage-specific K27) with ab181537 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear staining on lymphocytes of Rat spleen is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537)

    Intracellular flow cytometric analysis of2% paraformaldehyde-fixedJurkat (Human T cell leukemia cells from peripheral blood) cells labeling Ubiquitin (linkage-specific K27) with ab181537 at 1/160 dilution (red). The secondary antibody wasGoat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control isRabbit monoclonal IgG (black) and cell without incubation with primary antibody and secondary antibody is blue.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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