Rabbit Recombinant Monoclonal UBC Linkage-specific K27 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Synthetic peptide, Human, Mouse, Rat samples. Cited in 1 publication.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] - BSA and Azide free (AB238442), expandable thumbnail](https://content.abcam.com/products/images/ubiquitin-linkage-specific-k27-antibody-epr17034-bsa-and-azide-free-ab238442--immunocytochemistry-immunofluorescence-img14732.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] - BSA and Azide free (AB238442)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] - BSA and Azide free (AB238442), expandable thumbnail](https://content.abcam.com/products/images/ubiquitin-linkage-specific-k27-antibody-epr17034-bsa-and-azide-free-ab238442--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img78373.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] - BSA and Azide free (AB238442)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] - BSA and Azide free (AB238442), expandable thumbnail](https://content.abcam.com/products/images/ubiquitin-linkage-specific-k27-antibody-epr17034-bsa-and-azide-free-ab238442--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img62940.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] - BSA and Azide free (AB238442)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] - BSA and Azide free (AB238442), expandable thumbnail](https://content.abcam.com/products/images/ubiquitin-linkage-specific-k27-antibody-epr17034-bsa-and-azide-free-ab238442--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113286.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] - BSA and Azide free (AB238442)")
![Flow Cytometry (Intracellular) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] - BSA and Azide free (AB238442), expandable thumbnail](https://content.abcam.com/products/images/ubiquitin-linkage-specific-k27-antibody-epr17034-bsa-and-azide-free-ab238442--flow-cytometry-intracellular-img115227.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] - BSA and Azide free (AB238442)")
Publications
Filter by
- Nature communications 14:73482023An adaptive stress response that confers cellular resilience to decreased ubiquitination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiam C Hunt,Vishwajeeth Pagala,Anna Stephan,Boer Xie,Kiran Kodali,Kanisha Kavdia,Yong-Dong Wang,Abbas Shirinifard,Michelle Curley,Flavia A Graca,Yingxue Fu,Suresh Poudel,Yuxin Li,Xusheng Wang,Haiyan Tan,Junmin Peng,Fabio DemontisPubMed 37963875
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Expected | Tested | Tested |
| Mouse | Predicted | Expected | Predicted | Predicted |
| Rat | Predicted | Expected | Predicted | Predicted |
| Synthetic peptide | Tested | Expected | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes Actual Band: 14kDa and above. |
Species Human | Dilution info - | Notes Actual Band: 14kDa and above. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Actual Band: 14kDa and above. |
Species Rat | Dilution info - | Notes Actual Band: 14kDa and above. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide, Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Version
6 products for Alternative Product
Target data
Function
Ubiquitin. Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in proteotoxic stress response and cell cycle; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. During ubiquitination, the acceptor ubiquitin is positioned in the active site via direct interaction with the E2 ubiquitin-conjugating enzymes such as UBE2R2 (PubMed:38326650).
Alternative names
Polyubiquitin-C, UBC
Recommended products
Rabbit Recombinant Monoclonal UBC Linkage-specific K27 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Synthetic peptide, Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR17034
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab238442 is the carrier-free version of Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] ab181537.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Ubiquitin is a small regulatory protein found in almost all tissues of eukaryotic organisms. It has a molecular weight of approximately 8.6 kilodaltons. It functions mechanically by attaching to proteins through a process called ubiquitination which involves the formation of an isopeptide bond. Ubiquitin molecules can form polyubiquitin chains through different lysine residues such as K48 and K63 that determine their function. These chains label substrate proteins for various fates including degradation. Ubiquitin is expressed ubiquitously in cells reflecting its essential role in maintaining protein homeostasis.
- Biological function summary
The ubiquitin system plays a critical role in regulating protein turnover and quality control within cells. It is part of a larger complex known as the ubiquitin-proteasome system (UPS) which is responsible for degrading proteins that need to be turned over. This process is essential for cell cycle control response to oxidative stress and DNA repair. Ubiquitin's role in tagging proteins for degradation or signaling allows cells to respond quickly to changes in their environment and maintain balance.
- Pathways
Ubiquitin functions in several important biological pathways including the Wnt and NF-kB pathways. In the Wnt signaling pathway ubiquitination modulates the stability of key components thereby affecting the pathway's overall activity. In the NF-kB signaling pathway ubiquitin labels inhibitor proteins for degradation which releases and activates NF-kB. These pathways highlight ubiquitin's interaction with proteins such as beta-catenin in Wnt and IkB in NF-kB illustrating how it regulates diverse cellular processes.
- Associated diseases and disorders
The dysfunction of the ubiquitin system is linked to neurodegenerative diseases and cancers. Ubiquitin-related defects in protein degradation can lead to the buildup of unwanted proteins contributing to conditions like Parkinson's disease. Connections with cancer are evident as ubiquitin controls cell cycle proteins and aberrant ubiquitination may drive tumor growth and progression. The protein p53 known to be controlled by ubiquitination plays a significant role in cancer related mechanisms when dysregulated. Understanding and targeting ubiquitin-related pathways may provide new therapeutic opportunities for treating these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] - BSA and Azide free (ab238442), expandable thumbnail](https://content.abcam.com/products/images/ubiquitin-linkage-specific-k27-antibody-epr17034-bsa-and-azide-free-ab238442--immunocytochemistry-immunofluorescence-img14732.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] - BSA and Azide free (ab238442)")
Immunocytochemistry/ Immunofluorescence - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] - BSA and Azide free (ab238442)
Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma)
labelling Ubiquitin (linkage-specific K27) with purified Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] ab181537 at 1/1000. Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] ab181537).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] - BSA and Azide free (ab238442), expandable thumbnail](https://content.abcam.com/products/images/ubiquitin-linkage-specific-k27-antibody-epr17034-bsa-and-azide-free-ab238442--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img78373.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] - BSA and Azide free (ab238442)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] - BSA and Azide free (ab238442)
Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder labeling Ubiquitin (linkage-specific K27) with Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] ab181537 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear staining on cancer cells of Human bladder transitional cell carcinoma is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] ab181537).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] - BSA and Azide free (ab238442), expandable thumbnail](https://content.abcam.com/products/images/ubiquitin-linkage-specific-k27-antibody-epr17034-bsa-and-azide-free-ab238442--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img62940.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] - BSA and Azide free (ab238442)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] - BSA and Azide free (ab238442)
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Ubiquitin (linkage-specific K27) with Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] ab181537 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear staining on lymphocytes of Mouse spleen is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] ab181537).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] - BSA and Azide free (ab238442), expandable thumbnail](https://content.abcam.com/products/images/ubiquitin-linkage-specific-k27-antibody-epr17034-bsa-and-azide-free-ab238442--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113286.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] - BSA and Azide free (ab238442)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] - BSA and Azide free (ab238442)
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Ubiquitin (linkage-specific K27) with Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] ab181537 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear staining on lymphocytes of Rat spleen is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] ab181537).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] - BSA and Azide free (ab238442), expandable thumbnail](https://content.abcam.com/products/images/ubiquitin-linkage-specific-k27-antibody-epr17034-bsa-and-azide-free-ab238442--flow-cytometry-intracellular-img115227.jpg "Flow Cytometry (Intracellular) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] - BSA and Azide free (ab238442)")
Flow Cytometry (Intracellular) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] - BSA and Azide free (ab238442)
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling Ubiquitin (linkage-specific K27) with Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] ab181537 at 1/160 dilution (red). The secondary antibody was Goat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control is Rabbit monoclonal IgG (black) and cell without incubation with primary antibody and secondary antibody is blue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] ab181537).
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