Anti-Ubiquitin antibody (-K63) [EPR8590-448] ab179434 is a rabbit monoclonal antibody that is used in Ubiquitin western blotting, IHC and flow cytometry. Suitable for human, mouse and rat samples.
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
New 20 ul size available
Images
![Flow Cytometry (Intracellular) - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (AB179434), expandable thumbnail](https://content.abcam.com/products/images/ubiquitin-linkage-specific-k63-antibody-epr8590-448-ab179434--flow-cytometry-intracellular-img138389.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (AB179434)")
![Western blot - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (AB179434), expandable thumbnail](https://content.abcam.com/products/images/ubiquitin-linkage-specific-k63-antibody-epr8590-448-ab179434--western-blot-img129826.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (AB179434)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (AB179434), expandable thumbnail](https://content.abcam.com/products/images/ubiquitin-linkage-specific-k63-antibody-epr8590-448-ab179434--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img96234.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (AB179434)")
![Western blot - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (AB179434), expandable thumbnail](https://content.abcam.com/products/images/ubiquitin-linkage-specific-k63-antibody-epr8590-448-ab179434--western-blot-img105143.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (AB179434)")
![Western blot - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (AB179434), expandable thumbnail](https://content.abcam.com/products/images/ubiquitin-linkage-specific-k63-antibody-epr8590-448-ab179434--western-blot-img117843.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (AB179434)")
Publications
Filter by
- Molecular neurobiology 61:3258-32712023Electroacupuncture Inhibits Neuroinflammation Induced by Astrocytic Necroptosis Through RIP1/MLKL/TLR4 Pathway in a Mouse Model of Spinal Cord Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongdi Zhao,Xiaoqin Zong,Long Li,Na Li,Chunlei Liu,Wanchao Zhang,Juan Li,Cheng Yang,Siqin HuangPubMed 37982922
- Chinese medicine 18:1432023Xinyang tablet ameliorates sepsis-induced myocardial dysfunction by regulating Beclin-1 to mediate macrophage autophagy and M2 polarization through LncSICRNT1 targeting E3 ubiquitin ligase TRAF6.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuanyuan Luo,Yuanmei Li,Liwei He,Haitao Tu,Xinfeng Lin,Fengli Zhao,Yusheng Huang,Minyong Wen,Lingjun Wang,Zhongqi YangPubMed 37919806
- Cellular and molecular life sciences : CMLS 80:3362023Deubiquitinase OTUD5 promotes hepatitis B virus replication by removing K48-linked ubiquitination of HBV core/precore and upregulates HNF4ɑ expressions by inhibiting the ERK1/2/mitogen-activated protein kinase pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBin Lou,Guanghua Ma,Xiaopeng Yu,Feifei Lv,Fanjie Xu,Chengdi Sun,Yu ChenPubMed 37897511
- Experimental dermatology 32:1960-19702023Nuclear translocation of PKM2 mediates keratinocyte metabolic reprogramming in psoriasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuting Yang,Jie Zhang,Chunqing Hu,Xiaowen Chen,Yang Yang,Huihao Tang,Xiaolei Ding,Yaping YanPubMed 37688280
- Research (Washington, D.C.) 6:00912023SERBP1 Promotes Stress Granule Clearance by Regulating 26S Proteasome Activity and G3BP1 Ubiquitination and Protects Male Germ Cells from Thermostimuli Damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFengli Wang,Lingjuan Wang,Shiming Gan,Shenglei Feng,Sijin Ouyang,Xiaoli Wang,Shuiqiao YuanPubMed 37223481
- Cell reports 42:1121252023TRIM21-dependent target protein ubiquitination mediates cell-free Trim-Away.Applications:WBReactive species:Xenopus laevisTycho E T Mevissen,Anisa V Prasad,Johannes C WalterPubMed 36807144
- Autophagy 19:1803-18202023Endothelial TFEB signaling-mediated autophagic disturbance initiates microglial activation and cognitive dysfunction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYaping Lu,Xiang Chen,Xiuxiu Liu,Yi Shi,Zhaocong Wei,Lili Feng,Quan Jiang,Weifeng Ye,Takuya Sasaki,Kohji Fukunaga,Yong Ji,Feng Han,Ying-Mei LuPubMed 36588318
- Journal of experimental & clinical cancer research : CR 41:3362022TRIM56 promotes malignant progression of glioblastoma by stabilizing cIAP1 protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXu Yang,Yan Zhang,Zhiwei Xue,Yaotian Hu,Wenjing Zhou,Zhiyi Xue,Xuemeng Liu,Guowei Liu,Wenjie Li,Xiaofei Liu,Xingang Li,Mingzhi Han,Jian WangPubMed 36471347
- Virology journal 19:1762022Tripartite motif-containing protein 46 accelerates influenza A H7N9 virus infection by promoting K48-linked ubiquitination of TBK1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Su,Xian-Tian Lin,Shuai Zhao,Xiao-Qin Zheng,Yu-Qing Zhou,Lan-Lan Xiao,Hui Chen,Zheng-Yu Zhang,Li-Jun Zhang,Xiao-Xin WuPubMed 36329446
- The EMBO journal 41:e1089702022The E3 ubiquitin ligase RNF115 regulates phagosome maturation and host response to bacterial infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOrsolya Bilkei-Gorzo,Tiaan Heunis,José Luis Marín-Rubio,Francesca Romana Cianfanelli,Benjamin Bernard Armando Raymond,Joseph Inns,Daniela Fabrikova,Julien Peltier,Fiona Oakley,Ralf Schmid,Anetta Härtlova,Matthias TrostPubMed 36281581
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Tested | Expected | Expected |
| Recombinant full length protein | Tested | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/210 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 - 1/500 | Notes For unpurified, use 1/100 - 1/250. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
1 product for Alternative Version
Target data
Function
UbiquitinExists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
Alternative names
Polyubiquitin-C, UBC
Recommended products
Anti-Ubiquitin antibody (-K63) [EPR8590-448] ab179434 is a rabbit monoclonal antibody that is used in Ubiquitin western blotting, IHC and flow cytometry. Suitable for human, mouse and rat samples.
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
New 20 ul size available
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR8590-448
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Ubiquitin is a small regulatory protein found in almost all tissues of eukaryotic organisms. It has a molecular weight of approximately 8.6 kilodaltons. It functions mechanically by attaching to proteins through a process called ubiquitination which involves the formation of an isopeptide bond. Ubiquitin molecules can form polyubiquitin chains through different lysine residues such as K48 and K63 that determine their function. These chains label substrate proteins for various fates including degradation. Ubiquitin is expressed ubiquitously in cells reflecting its essential role in maintaining protein homeostasis.
- Biological function summary
The ubiquitin system plays a critical role in regulating protein turnover and quality control within cells. It is part of a larger complex known as the ubiquitin-proteasome system (UPS) which is responsible for degrading proteins that need to be turned over. This process is essential for cell cycle control response to oxidative stress and DNA repair. Ubiquitin's role in tagging proteins for degradation or signaling allows cells to respond quickly to changes in their environment and maintain balance.
- Pathways
Ubiquitin functions in several important biological pathways including the Wnt and NF-kB pathways. In the Wnt signaling pathway ubiquitination modulates the stability of key components thereby affecting the pathway's overall activity. In the NF-kB signaling pathway ubiquitin labels inhibitor proteins for degradation which releases and activates NF-kB. These pathways highlight ubiquitin's interaction with proteins such as beta-catenin in Wnt and IkB in NF-kB illustrating how it regulates diverse cellular processes.
- Associated diseases and disorders
The dysfunction of the ubiquitin system is linked to neurodegenerative diseases and cancers. Ubiquitin-related defects in protein degradation can lead to the buildup of unwanted proteins contributing to conditions like Parkinson's disease. Connections with cancer are evident as ubiquitin controls cell cycle proteins and aberrant ubiquitination may drive tumor growth and progression. The protein p53 known to be controlled by ubiquitination plays a significant role in cancer related mechanisms when dysregulated. Understanding and targeting ubiquitin-related pathways may provide new therapeutic opportunities for treating these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Flow Cytometry (Intracellular) - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434), expandable thumbnail](https://content.abcam.com/products/images/ubiquitin-linkage-specific-k63-antibody-epr8590-448-ab179434--flow-cytometry-intracellular-img138389.jpg "Flow Cytometry (Intracellular) - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434)")
Flow Cytometry (Intracellular) - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434)
Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling Ubiquitin (linkage-specific K63) with purified ab179434 at 1/210 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. An Alexa Fluor®488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
![Western blot - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434), expandable thumbnail](https://content.abcam.com/products/images/ubiquitin-linkage-specific-k63-antibody-epr8590-448-ab179434--western-blot-img129826.jpg "Western blot - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434)")
Western blot - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434) at 1/1000 dilution
Lane 1: HEK-293 (human epithelial cell line from embryonic kidney) cell lysate at 20 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg
SecondaryAll lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 26 kDa
Observed band size: 16-300 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434), expandable thumbnail](https://content.abcam.com/products/images/ubiquitin-linkage-specific-k63-antibody-epr8590-448-ab179434--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img96234.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434)
Immunohistochemical staining of paraffin embedded human tonsil with purified ab179434 at a dilution of 1/500. The secondary antibody used is a HRP conjugated goat anti-rabbit (Goat Anti-Rabbit IgG H&L (HRP) ab97051, 1/500). The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
![Western blot - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434), expandable thumbnail](https://content.abcam.com/products/images/ubiquitin-linkage-specific-k63-antibody-epr8590-448-ab179434--western-blot-img105143.jpg "Western blot - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434)")
Western blot - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434) at 1/5000 dilution
Lane 1: K6-linked-Ub2 recombinant protein at 20 µg
Lane 2: K11-linked-Ub2 recombinant protein at 20 µg
Lane 3: K29-linked-Ub2 recombinant protein at 20 µg
Lane 4: K33-linked-Ub2 recombinant protein at 20 µg
Lane 5: K48-linked-Ub2 recombinant protein at 20 µg
Lane 6: K63-linked-Ub2 recombinant protein at 20 µg
SecondaryAll lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 26 kDa
Observed band size: 16-70 kDa
![Western blot - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434), expandable thumbnail](https://content.abcam.com/products/images/ubiquitin-linkage-specific-k63-antibody-epr8590-448-ab179434--western-blot-img117843.jpg "Western blot - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434)")
Western blot - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Rat brain tissue lysate at 20 µg
SecondaryAll lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 26 kDa
Observed band size: 16-300 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434), expandable thumbnail](https://content.abcam.com/products/images/ubiquitin-linkage-specific-k63-antibody-epr8590-448-ab179434--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111209.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434)
Immunohistochemical analysis of paraffin-embedded human kidney carcinoma tissue labeling K63-linkage specific polyubiquitin with unpurified ab179434 at 1/100 dilution.
![Western blot - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434), expandable thumbnail](https://content.abcam.com/products/images/ubiquitin-linkage-specific-k63-antibody-epr8590-448-ab179434--western-blot-img107857.jpg "Western blot - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434)")
Western blot - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434)
All lanes: Western blot - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434) at 1/1000 dilution
Lane 1: Ubiquitin recombinant protein at 0.02 µg
Lane 2: K6-linked-Ub2 recombinant protein at 0.02 µg
Lane 3: K11-linked-Ub2 recombinant protein at 0.02 µg
Lane 4: K27-linked-Ub2 recombinant protein at 0.02 µg
Lane 5: K29-linked-Ub2 recombinant protein at 0.02 µg
Lane 6: K33-linked-Ub2 recombinant protein at 0.02 µg
Lane 7: K48-linked-Ub2-7 recombinant protein at 0.02 µg
Lane 8: K63-linked-Ub2-7 recombinant protein at 0.02 µg
Predicted band size: 26 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434), expandable thumbnail](https://content.abcam.com/products/images/ubiquitin-linkage-specific-k63-antibody-epr8590-448-ab179434--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111209.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434)")
![Western blot - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434), expandable thumbnail](https://content.abcam.com/products/images/ubiquitin-linkage-specific-k63-antibody-epr8590-448-ab179434--western-blot-img107857.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com