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AB285010

Anti-Ubiquitin+1/UBB+1 antibody [EPR24825-103] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal UBB mutated antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

Polyubiquitin-B, UBB

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Sandwich ELISA - Anti-Ubiquitin+1/UBB+1 antibody [EPR24825-103] - BSA and Azide free (Capture) (AB285010)
  • sELISA

Lab

Sandwich ELISA - Anti-Ubiquitin+1/UBB+1 antibody [EPR24825-103] - BSA and Azide free (Capture) (AB285010)

Sandwich ELISA of ab253843 with the capture antibody ab285010 dilution at 2 µg/mL and detector antibody ab285116 dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24825-103

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab285010 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Tetra-ubiquitin. The recommended pair for sandwich ELISA is:
Capture: ab285010, Human Ubiquitin +1 Capture Antibody (unconjugated)
Detector: ab285116, Human Ubiquitin Detector Antibody (unconjugated)
The reference range value is 31.25-2000 pg/mL.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ubiquitin+1 (UBB+1) is an altered form of the ubiquitin protein which plays a significant role in protein degradation. This mutant ubiquitin results from a molecular error during transcription known as a frameshift mutation. The mass of UBB+1 is slightly greater than that of the normal ubiquitin due to an extra amino acid. UBB+1 is expressed primarily in neuronal cells where it accumulates due to its impaired function in tagging proteins for degradation by the proteasome.
Biological function summary

The alteration in UBB+1 affects the cellular protein quality control system. UBB+1 cannot be recognized properly for degradation itself and hinders the ubiquitin-proteasome system (UPS) by competing with normal ubiquitin. UBB+1 does not just influence single proteins; it might also intervene in larger complexes related to proteostasis. Its aberrant accumulation disrupts the clearance of damaged or misfolded proteins which can impact cellular health.

Pathways

Ubiquitin+1 affects several fundamental cellular processes by disturbing the UPS pathway. UBB+1 interacts with proteins involved in the ubiquitin-proteasome pathway such as E1 activating enzymes E2 conjugating enzymes and E3 ligases. Through these associations UBB+1 can induce stress in protein turnover and impair cellular regulation with direct implications in the processes related to protein homeostasis. The effect on these pathways can lead to wider disruptions in cellular signaling and maintenance.

Ubiquitin+1 has a notable impact on neurodegenerative diseases particularly Alzheimer's disease. It is associated with the accumulation of neurofibrillary tangles and plaques seen in these conditions. UBB+1 also connects with proteins like amyloid-beta which play central roles in Alzheimer's pathology. Furthermore UBB+1 accumulation has implications in Huntington’s disease where it links with the aggregation-prone mutant huntingtin protein. These associations highlight the significance of UBB+1 in disease mechanisms involving protein aggregation and cellular stress responses.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ubiquitin. Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked : Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in proteotoxic stress response and cell cycle; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
See full target information Ubiquitin+1/UBB+1 mutated
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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