JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB176340

Anti-UBLCP1 antibody [EPR11923]

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal UBLCP1 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Ubiquitin-like domain-containing CTD phosphatase 1, Nuclear proteasome inhibitor UBLCP1, UBLCP1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-UBLCP1 antibody [EPR11923] (AB176340)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-UBLCP1 antibody [EPR11923] (AB176340)

Immunofluorescence analysis of HeLa cells labeling UBLCP1 with ab176340 at 1/50 dilution.

Immunoprecipitation - Anti-UBLCP1 antibody [EPR11923] (AB176340)
  • IP

Supplier Data

Immunoprecipitation - Anti-UBLCP1 antibody [EPR11923] (AB176340)

Western blot analysis on immunoprecipitation pellet from K562 cell lysate using ab176340 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-UBLCP1 antibody [EPR11923] (ab176340)

Predicted band size: 37 kDa

false

Western blot - Anti-UBLCP1 antibody [EPR11923] (AB176340)
  • WB

Supplier Data

Western blot - Anti-UBLCP1 antibody [EPR11923] (AB176340)

All lanes:

Western blot - Anti-UBLCP1 antibody [EPR11923] (ab176340) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Predicted band size: 37 kDa

false

  • Carrier free

    Anti-UBLCP1 antibody [EPR11923] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11923

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UBLCP1 also known as Ubiquitin-Like Domain Containing CTD Phosphatase 1 is a phosphatase that primarily functions in dephosphorylation of substrate proteins. This protein has a molecular mass of approximately 44 kDa and is expressed in various human tissues but shows higher levels in the brain and skeletal muscle. UBLCP1 contains ubiquitin-like domains which indicate its potential interactions with other molecules containing ubiquitin or ubiquitin-like proteins influencing its mechanical roles inside cells.
Biological function summary

UBLCP1 plays a significant role in regulating cellular processes by removing phosphate groups from substrates which can affect protein activity cellular localization or interaction with other proteins. It does not work alone but associates with other molecules to form complexes that allow it to control different cellular processes effectively. One such interaction is with the RNA polymerase II where UBLCP1 may regulate transcription by dephosphorylating the C-terminal domain of RNA polymerase II.

Pathways

Several studies have linked UBLCP1 to key signaling pathways that control cell cycle and transcription regulation. It interacts with pathways involving RNA polymerase II influencing its activity during transcription. Additionally UBLCP1 shares interactions with components of the TGF-beta pathway though details of this relationship require further study. This connection could suggest a role in controlling growth and differentiation signals in cells.

Researchers associate UBLCP1 with neurodegenerative diseases including Alzheimer's disease given its higher expression in brain tissues and involvement in transcriptional regulation. Perturbations in UBLCP1's activity could affect proteins related to synaptic function and memory. Moreover UBLCP1 interaction with proteins like RNA polymerase II indicates that it may influence diseases where transcription is disrupted such as certain cancers. Further understanding of its mechanistic role could provide insights into novel therapeutic strategies.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Dephosphorylates 26S nuclear proteasomes, thereby decreasing their proteolytic activity (PubMed : 21949367, PubMed : 28539385). Recruited to the 19S regulatory particle of the 26S proteasome through its interaction with 19S component PSMD2/RPN1 (PubMed : 28539385). Once recruited, dephosphorylates 19S component PSMC2/RPT1 which impairs PSMC2 ATPase activity and disrupts 26S proteasome assembly (PubMed : 28539385). Has also been reported to stimulate the proteolytic activity of the 26S proteasome (PubMed : 32071216).
See full target information UBLCP1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Open biology 7: PubMed28539385

2017

Phosphatase UBLCP1 controls proteasome assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Shuangwu Sun,Sisi Liu,Zhengmao Zhang,Wang Zeng,Chuang Sun,Tao Tao,Xia Lin,Xin-Hua Feng
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB235378

Anti-UBLCP1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBLCP1 antibody (AB235378)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB192618

Anti-USP14/TGT antibody [EPR15943] - C-terminal

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-USP14/TGT antibody [EPR15943] - C-terminal (AB192618)

  • 5
  • 1
Primary Antibodies

AB176689

Anti-ZNF384 antibody

Immunoprecipitation - Anti-ZNF384 antibody (AB176689)

  • 5
  • 1
Primary Antibodies

AB314544

Anti-USP48 antibody [EPR26175-11] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP48 antibody [EPR26175-11] - BSA and Azide free (AB314544)

  • 0
  • 0
Primary Antibodies

AB133323

Anti-14-3-3 antibody [EPR6379]

RabMAb|Recombinant

Western blot - Anti-14-3-3 antibody [EPR6379] (AB133323)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com