Rabbit Polyclonal UBN1 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human UBN1 aa 1050 to C-terminus.
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Publications
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- Nature structural & molecular biology 27:1152-11642020Regulation of ALT-associated homology-directed repair by polyADP-ribosylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSong My Hoang et. al.PubMed 33046907
- Nature structural & molecular biology 27:1057-10682020Two HIRA-dependent pathways mediate H3.3 de novo deposition and recycling during transcription.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJúlia Torné,Dominique Ray-Gallet,Ekaterina Boyarchuk,Mickaël Garnier,Patricia Le Baccon,Antoine Coulon,Guillermo A Orsi,Geneviève AlmouzniPubMed 32895554
- Nature communications 9:31032018Functional activity of the H3.3 histone chaperone complex HIRA requires trimerization of the HIRA subunit.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDominique Ray-Gallet et. al.PubMed 30082790
- Molecular endocrinology (Baltimore, Md.) 29:1426-392015Identification of a Novel Coregulator, SH3YL1, That Interacts With the Androgen Receptor N-Terminus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlicia M Blessing et. al.PubMed 26305679
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human UBN1 aa 1050 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NPG3
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Chimpanzee | Predicted |
| Gorilla | Predicted |
| Rhesus monkey | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Rhesus monkey, Gorilla | Dilution info - | Notes - |
Target data
Function
Acts as a novel regulator of senescence. Involved in the formation of senescence-associated heterochromatin foci (SAHF), which represses expression of proliferation-promoting genes. Binds to proliferation-promoting genes. May be required for replication-independent chromatin assembly.
Alternative names
Ubinuclein-1, HIRA-binding protein, Protein VT4, Ubiquitously expressed nuclear protein, UBN1
Recommended products
Rabbit Polyclonal UBN1 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human UBN1 aa 1050 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human UBN1 aa 1050 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NPG3
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab101282 was affinity purified using an epitope specific to UBN1 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
UBN1 also known as Ubinuclein-1 plays an important role at the mechanical level in remodeling chromatin structure. It has a mass of approximately 83 kDa. This protein is found predominantly in the nucleus and is widespread across different tissues particularly in tissues with higher cellular turnover. UBN1 works by interacting with other chromatin-associated proteins such as histones to facilitate chromatin alterations that are necessary for various cellular processes.
- Biological function summary
UBN1 acts as a participant in the regulation of gene expression. It is a component of the Human Histone Acetyltransferase complex (HBO1 complex) which modifies chromatin by adding acetyl groups to histones enabling a more open chromatin state. This more open state is important for gene transcription and DNA replication. UBN1's role in this complex is significant for the regulation of genes important for cell cycle progression and other essential cellular functions.
- Pathways
Various control processes converge at UBN1. It plays a significant role in the epigenetic pathway influencing gene expression through chromatin modification. UBN1 is involved in the DNA replication initiation pathway where it facilitates the assembly of pre-replicative complexes. In these pathways UBN1 is related to proteins such as MCM2-7 which are essential for unwinding DNA during replication.
- Associated diseases and disorders
Aberrant UBN1 activity has been linked to cancer and neurodevelopmental disorders. In cancer dysregulated expression of UBN1 can lead to improper gene regulation contributing to uncontrolled cell growth and proliferation. UBN1 interacts with proteins like c-Myc in oncogenic pathways. Additionally changes in UBN1 function have been associated with neurodevelopmental disorders through its influence on chromatin dynamics affecting genes essential for brain development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-UBN1 antibody (ab101282)
All lanes: Western blot - Anti-UBN1 antibody (ab101282) at 0.1 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 5 µg
Lane 4: 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 122 kDa
Exposure time: 3min
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