AB314066

Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free

Recombinant
KO Validated
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Rabbit Recombinant Monoclonal CIP75 antibody. Suitable for Flow Cyt, ICC, IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human UBQLN4.

View Alternative Names

C1orf6, CIP75, UBIN, UBQLN4, Ubiquilin-4, Ataxin-1 interacting ubiquitin-like protein, Ataxin-1 ubiquitin-like-interacting protein A1U, Connexin43-interacting protein of 75 kDa, A1Up

6 Images

Flow Cytometry - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (AB314066)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (AB314066)

Detection of human UBQLN4/CIP75/Ubiquilin 4 (shaded) in HeLa cells by flow cytometry. Antibody : Rabbit anti-UBQLN4 recombinant monoclonal antibody [BLR0145J] (A700-145) or isotype control (unshaded). Secondary : conjugated goat anti-rabbit IgG.

Supplier Data

Immunohistochemistry - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (AB314066)

Detection of human UBQLN4 in FFPE lung carcinoma by immunohistochemistry. Antibody : Rabbit anti-UBQLN4 recombinant monoclonal antibody [BLR145J] Secondary : HRP-conjugated goat anti-rabbit IgG . Substrate : DAB.

Supplier Data

Immunocytochemistry - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (AB314066)

Detection of human UBQLN4 in FFPE Ovcar-4 cells by immunocytochemistry Antibody : Rabbit anti-UBQLN4 recombinant monoclonal antibody [BLR145J] Secondary : HRP-conjugated goat anti-rabbit IgG . Substrate : DAB.

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Immunoprecipitation - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (AB314066)

Samples : Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies : Rabbit antiUBQLN4 recombinant monoclonal antibody [BLR145J] (A700-145 lot 1) used for IP at 20 µl/mg lysate. UBQLN4 was also immunoprecipitated by others rabbit anti-UBQLN4 antibodies (A305-237A and BL-1677B-1B8). For blotting immunoprecipitated UBQLN4, A700-145 was used at 1 : 1000. Chemiluminescence with an exposure time of 10 seconds.

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Supplier Data

Western blot - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (AB314066)

Samples : Whole cell lysate (50 µg) prepared using NETN buffer from MDA-MB-231 cells (WT and KO). Antibodies : Rabbit antiUBQLN4 recombinant monoclonal antibody [BLR145J] (A700-145 lot 1) used for WB at 1 : 1000. Secondary : HRPconjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 30 seconds. Lower Panel : Rabbit anti-COPB2 antibody (A304-523A). (KO cells : Jachimowicz et al, Cell, 2019).

All lanes:

Western blot - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (ab314066) at 1/1000 dilution

Lane 1:

WT Whole cell lysate at 50 µg

Lane 2:

WT KO Whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

false

Supplier Data

Western blot - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (AB314066)

Samples : Whole cell lysate (50 µg) from HEK293T, OVCAR-3, MCF-7, and K-562 cells prepared using NETN lysis buffer. Antibody : Rabbit anti-UBQLN4 recombinant monoclonal antibody [BLR145J] (A700-145 lot

used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 3 seconds. Lower Panel : Rabbit anti-COPB2 antibody (A304-523A).

All lanes:

Western blot - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (ab314066) at 1/1000 dilution

Lane 1:

HEK293T Whole cell lysate at 50 µg

Lane 2:

OVCAR-3 Whole cell lysate at 50 µg

Lane 3:

MCF-7 Whole cell lysate at 50 µg

Lane 4:

K-562 Whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR145J

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, Flow Cyt, IP, ICC

applications

Immunogen

Recombinant Full Length Protein corresponding to Human UBQLN4.

Q9NRR5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulator of protein degradation that mediates the proteasomal targeting of misfolded, mislocalized or accumulated proteins (PubMed : 15280365, PubMed : 27113755, PubMed : 29666234, PubMed : 30612738). Acts by binding polyubiquitin chains of target proteins via its UBA domain and by interacting with subunits of the proteasome via its ubiquitin-like domain (PubMed : 15280365, PubMed : 27113755, PubMed : 30612738). Key regulator of DNA repair that represses homologous recombination repair : in response to DNA damage, recruited to sites of DNA damage following phosphorylation by ATM and acts by binding and removing ubiquitinated MRE11 from damaged chromatin, leading to MRE11 degradation by the proteasome (PubMed : 30612738). MRE11 degradation prevents homologous recombination repair, redirecting double-strand break repair toward non-homologous end joining (NHEJ) (PubMed : 30612738). Specifically recognizes and binds mislocalized transmembrane-containing proteins and targets them to proteasomal degradation (PubMed : 27113755). Collaborates with DESI1/POST in the export of ubiquitinated proteins from the nucleus to the cytoplasm (PubMed : 29666234). Also plays a role in the regulation of the proteasomal degradation of non-ubiquitinated GJA1 (By similarity). Acts as an adapter protein that recruits UBQLN1 to the autophagy machinery (PubMed : 23459205). Mediates the association of UBQLN1 with autophagosomes and the autophagy-related protein LC3 (MAP1LC3A/B/C) and may assist in the maturation of autophagosomes to autolysosomes by mediating autophagosome-lysosome fusion (PubMed : 23459205).

See full target information UBQLN4

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free

Flow Cytometry - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (AB314066)

Immunohistochemistry - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (AB314066)

Immunocytochemistry - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (AB314066)

Immunoprecipitation - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (AB314066)

Western blot - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (AB314066)

Western blot - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (AB314066)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise