Rabbit Recombinant Monoclonal CIP75 antibody. Suitable for Flow Cyt, ICC, IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human UBQLN4.
Images
![Flow Cytometry - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (AB314066), expandable thumbnail](https://content.abcam.com/products/images/ubqln4-cip75-ubiquilin-4-antibody-blr145j-bsa-free-ab314066--flow-cytometry-img420480.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (AB314066)")
![Western blot - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (AB314066), expandable thumbnail](https://content.abcam.com/products/images/ubqln4-cip75-ubiquilin-4-antibody-blr145j-bsa-free-ab314066--western-blot-img420476.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (AB314066)")
![Western blot - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (AB314066), expandable thumbnail](https://content.abcam.com/products/images/ubqln4-cip75-ubiquilin-4-antibody-blr145j-bsa-free-ab314066--western-blot-img420475.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (AB314066)")
![Immunoprecipitation - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (AB314066), expandable thumbnail](https://content.abcam.com/products/images/ubqln4-cip75-ubiquilin-4-antibody-blr145j-bsa-free-ab314066--immunoprecipitation-img420477.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (AB314066)")
![Immunocytochemistry - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (AB314066), expandable thumbnail](https://content.abcam.com/products/images/ubqln4-cip75-ubiquilin-4-antibody-blr145j-bsa-free-ab314066--immunocytochemistry-img420478.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (AB314066)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human UBQLN4. Database link Q9NRR5
Reactivity data
Select an application| Flow Cyt | ICC | IHC-P | IP | WB | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Regulator of protein degradation that mediates the proteasomal targeting of misfolded, mislocalized or accumulated proteins (PubMed:15280365, PubMed:27113755, PubMed:29666234, PubMed:30612738). Acts by binding polyubiquitin chains of target proteins via its UBA domain and by interacting with subunits of the proteasome via its ubiquitin-like domain (PubMed:15280365, PubMed:27113755, PubMed:30612738). Key regulator of DNA repair that represses homologous recombination repair: in response to DNA damage, recruited to sites of DNA damage following phosphorylation by ATM and acts by binding and removing ubiquitinated MRE11 from damaged chromatin, leading to MRE11 degradation by the proteasome (PubMed:30612738). MRE11 degradation prevents homologous recombination repair, redirecting double-strand break repair toward non-homologous end joining (NHEJ) (PubMed:30612738). Specifically recognizes and binds mislocalized transmembrane-containing proteins and targets them to proteasomal degradation (PubMed:27113755). Collaborates with DESI1/POST in the export of ubiquitinated proteins from the nucleus to the cytoplasm (PubMed:29666234). Also plays a role in the regulation of the proteasomal degradation of non-ubiquitinated GJA1 (By similarity). Acts as an adapter protein that recruits UBQLN1 to the autophagy machinery (PubMed:23459205). Mediates the association of UBQLN1 with autophagosomes and the autophagy-related protein LC3 (MAP1LC3A/B/C) and may assist in the maturation of autophagosomes to autolysosomes by mediating autophagosome-lysosome fusion (PubMed:23459205).
Alternative names
C1orf6, CIP75, UBIN, UBQLN4, Ubiquilin-4, Ataxin-1 interacting ubiquitin-like protein, Ataxin-1 ubiquitin-like-interacting protein A1U, Connexin43-interacting protein of 75 kDa, A1Up
Recommended products
Rabbit Recombinant Monoclonal CIP75 antibody. Suitable for Flow Cyt, ICC, IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human UBQLN4.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human UBQLN4. Database link Q9NRR5
- Clone number
- BLR145J
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- Up to 12 months
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Flow Cytometry - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (ab314066), expandable thumbnail](https://content.abcam.com/products/images/ubqln4-cip75-ubiquilin-4-antibody-blr145j-bsa-free-ab314066--flow-cytometry-img420480.jpg "Flow Cytometry - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (ab314066)")
Flow Cytometry - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (ab314066)
Detection of human UBQLN4/CIP75/Ubiquilin 4 (shaded) in HeLa cells by flow cytometry. Antibody: Rabbit anti-UBQLN4 recombinant monoclonal antibody [BLR0145J] (A700-145) or isotype control (unshaded). Secondary: conjugated goat anti-rabbit IgG.
![Western blot - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (ab314066), expandable thumbnail](https://content.abcam.com/products/images/ubqln4-cip75-ubiquilin-4-antibody-blr145j-bsa-free-ab314066--western-blot-img420476.jpg "Western blot - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (ab314066)")
Western blot - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (ab314066)
Samples: Whole cell lysate (50 µg) prepared using NETN buffer from MDA-MB-231 cells (WT and KO). Antibodies:Rabbit antiUBQLN4 recombinant monoclonal antibody [BLR145J] (A700-145 lot 1) used for WB at 1:1000. Secondary:HRPconjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody (A304-523A). (KO cells: Jachimowicz et al, Cell, 2019).
All lanes: Western blot - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (ab314066) at 1/1000 dilution
Lane 1: WT Whole cell lysate at 50 µg
Lane 2: WT KO Whole cell lysate at 50 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit IgG
![Western blot - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (ab314066), expandable thumbnail](https://content.abcam.com/products/images/ubqln4-cip75-ubiquilin-4-antibody-blr145j-bsa-free-ab314066--western-blot-img420475.jpg "Western blot - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (ab314066)")
Western blot - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (ab314066)
Samples: Whole cell lysate (50 µg) from HEK293T, OVCAR-3, MCF-7, and K-562 cells prepared using NETN lysis buffer. Antibody:Rabbit anti-UBQLN4 recombinant monoclonal antibody [BLR145J] (A700-145 lot 1) used at 1:1000. Secondary:HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-COPB2 antibody (A304-523A).
All lanes: Western blot - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (ab314066) at 1/1000 dilution
Lane 1: HEK293T Whole cell lysate at 50 µg
Lane 2: OVCAR-3 Whole cell lysate at 50 µg
Lane 3: MCF-7 Whole cell lysate at 50 µg
Lane 4: K-562 Whole cell lysate at 50 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit IgG
![Immunoprecipitation - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (ab314066), expandable thumbnail](https://content.abcam.com/products/images/ubqln4-cip75-ubiquilin-4-antibody-blr145j-bsa-free-ab314066--immunoprecipitation-img420477.jpg "Immunoprecipitation - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (ab314066)")
Immunoprecipitation - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (ab314066)
Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies:Rabbit antiUBQLN4 recombinant monoclonal antibody [BLR145J] (A700-145 lot 1) used for IP at 20 µl/mg lysate. UBQLN4 was also immunoprecipitated by others rabbit anti-UBQLN4 antibodies (A305-237A and BL-1677B-1B8). For blotting immunoprecipitated UBQLN4, A700-145 was used at 1:1000. Chemiluminescence with an exposure time of 10 seconds.
![Immunocytochemistry - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (ab314066), expandable thumbnail](https://content.abcam.com/products/images/ubqln4-cip75-ubiquilin-4-antibody-blr145j-bsa-free-ab314066--immunocytochemistry-img420478.jpg "Immunocytochemistry - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (ab314066)")
Immunocytochemistry - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (ab314066)
Detection of human UBQLN4 in FFPE Ovcar-4 cells by immunocytochemistry Antibody:Rabbit anti-UBQLN4 recombinant monoclonal antibody [BLR145J] Secondary:HRP-conjugated goat anti-rabbit IgG . Substrate:DAB.
![Immunohistochemistry - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (ab314066), expandable thumbnail](https://content.abcam.com/products/images/ubqln4-cip75-ubiquilin-4-antibody-blr145j-bsa-free-ab314066--immunohistochemistry-img420479.jpg "Immunohistochemistry - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (ab314066)")
Immunohistochemistry - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (ab314066)
Detection of human UBQLN4 in FFPE lung carcinoma by immunohistochemistry. Antibody:Rabbit anti-UBQLN4 recombinant monoclonal antibody [BLR145J] Secondary:HRP-conjugated goat anti-rabbit IgG . Substrate:DAB.
![Immunohistochemistry - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (ab314066), expandable thumbnail](https://content.abcam.com/products/images/ubqln4-cip75-ubiquilin-4-antibody-blr145j-bsa-free-ab314066--immunohistochemistry-img420479.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-UBQLN4/CIP75/Ubiquilin 4 antibody [BLR145J] - BSA free (ab314066)")
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