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AB169188

Anti-UBR3 antibody

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Mouse Polyclonal UBR3 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human UBR3 aa 1100 to C-terminus.

View Alternative Names

KIAA2024, ZNF650, UBR3, E3 ubiquitin-protein ligase UBR3, N-recognin-3, RING-type E3 ubiquitin transferase UBR3, Ubiquitin-protein ligase E3-alpha-3, Ubiquitin-protein ligase E3-alpha-III, Zinc finger protein 650

1 Images
Western blot - Anti-UBR3 antibody (AB169188)
  • WB

Unknown

Western blot - Anti-UBR3 antibody (AB169188)

All lanes:

Western blot - Anti-UBR3 antibody (ab169188) at 1 µg/mL

Lane 1:

UBR3 transfected 293T cell lysate (81.51 KDa) at 15 µL

Lane 2:

Non-transfected 293T cell lysate at 15 µL

Predicted band size: 14 kDa,212 kDa,22 kDa

Observed band size: 25 kDa,7 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human UBR3 aa 1100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q6ZT12

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: 99% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UBR3 also known as UBR domain-containing protein 3 functions as an important E3 ubiquitin ligase within the N-end rule pathway. It has a molecular mass of approximately 200 kDa. UBR3 is widely expressed with significant expression in the brain heart and skeletal muscle tissues. The protein plays an important role in recognizing and tagging proteins that display destabilizing N-terminal residues for proteasomal degradation a process essential for maintaining protein homeostasis.
Biological function summary

This protein performs essential roles in protein quality control and turnover. UBR3 is part of the ubiquitin-proteasome system and forms part of larger ubiquitin ligase complexes. This system governs the degradation of most intracellular proteins regulating various cellular processes like cell cycle progression and apoptosis. It ensures that damaged or misfolded proteins which could disrupt cellular functions are promptly identified and degraded.

Pathways

The ubiquitin proteasome pathway is one of the most important biological frameworks involving UBR3. This pathway pivotal for regulating protein concentrations and controlling cellular functions connects UBR3 with other proteins such as UBR1 and UBR2 which together function in substrate recognition and ubiquitination. Additionally UBR3 is involved in the N-end rule pathway further tying it to numerous cellular activities related to protein synthesis modification and degradation.

Irregularities in UBR3 function could contribute to neurological conditions and muscular degenerative disorders. Altered activity or expression of UBR3 might lead to Neurological disorders such as certain types of ataxias possibly due to disrupted protein homeostasis in neurons. Connections with proteins like p53 in pathways regulated by ubiquitin ligases also implicate UBR3 in cancer where it could affect cell cycle control and apoptosis thereby influencing tumor growth and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase which is a component of the N-end rule pathway (By similarity). Does not bind to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation (By similarity). May play a role in Shh signaling by mediating the ubiquitination of Kif7 (By similarity). May be important for MYH9 function in certain tissues, possibly by regulating the ubiquitination of MYH9 and consequently affecting its interaction with MYO7A (PubMed : 27331610).
See full target information UBR3
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