Rabbit Recombinant Monoclonal UBR7 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra), IP and reacts with Human, Mouse, Rat samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (AB305240), expandable thumbnail](https://content.abcam.com/products/images/ubr7-antibody-epr26170-67-bsa-and-azide-free-ab305240--immunocytochemistry-immunofluorescence-img403902.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (AB305240)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (AB305240), expandable thumbnail](https://content.abcam.com/products/images/ubr7-antibody-epr26170-67-bsa-and-azide-free-ab305240--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403827.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (AB305240)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (AB305240), expandable thumbnail](https://content.abcam.com/products/images/ubr7-antibody-epr26170-67-bsa-and-azide-free-ab305240--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403824.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (AB305240)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (AB305240), expandable thumbnail](https://content.abcam.com/products/images/ubr7-antibody-epr26170-67-bsa-and-azide-free-ab305240--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403825.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (AB305240)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (AB305240), expandable thumbnail](https://content.abcam.com/products/images/ubr7-antibody-epr26170-67-bsa-and-azide-free-ab305240--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403826.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (AB305240)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt (Intra) | IP | ChIP | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested | Not recommended |
| Mouse | Tested | Tested | Tested | Tested | Tested | Not recommended |
| Rat | Tested | Tested | Expected | Expected | Expected | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ChIP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.
Alternative names
Putative E3 ubiquitin-protein ligase UBR7, N-recognin-7, RING-type E3 ubiquitin transferase UBR7, C14orf130, UBR7
Recommended products
Rabbit Recombinant Monoclonal UBR7 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra), IP and reacts with Human, Mouse, Rat samples.
Alternative names
Putative E3 ubiquitin-protein ligase UBR7, N-recognin-7, RING-type E3 ubiquitin transferase UBR7, C14orf130, UBR7
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR26170-67
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
UBR7 also known as E3 ubiquitin-protein ligase UBR7 is a protein encoded by the UBR7 gene. The molecular mass of UBR7 is approximately 82 kDa. This protein is expressed in various human tissues with notable higher levels in the liver kidney and heart. Mechanically UBR7 functions as an E3 ubiquitin ligase which means it plays a role in attaching ubiquitin molecules to specific substrates marking them for degradation by the proteasome.
- Biological function summary
UBR7 acts within cellular protein quality control by ensuring misfolded or damaged proteins are ubiquitinated for degradation. It is part of a larger ubiquitin-proteasome system which tightly controls protein turnover and homeostasis within the cell. This system helps maintain cell functions by regulating the abundance of key proteins involved in various cellular processes such as signal transduction and cell cycle control.
- Pathways
The ubiquitin-proteasome system where UBR7 plays a significant role intersects with the cell cycle regulation and DNA damage response pathways. UBR7 interacts with proteins like p53 a well-known tumor suppressor playing a regulatory role in its stability and activity. Additionally it is involved in pathways concerning apoptosis contributing to programmed cell death when abnormal cells are detected.
- Associated diseases and disorders
Aberrations in UBR7 activity have been linked to cancer particularly related to disruptions in the regulation of p53. The dysregulation can lead to uncontrolled cell growth and tumor development. UBR7 also associates with neurodegenerative disorders where the accumulation of misfolded proteins due to impaired ubiquitination contributes to neuronal damage. Here its connection with proteins such as Tau is significant as abnormal ubiquitination patterns have been observed in cases of Alzheimer's disease.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
13 product images
![Immunocytochemistry/ Immunofluorescence - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240), expandable thumbnail](https://content.abcam.com/products/images/ubr7-antibody-epr26170-67-bsa-and-azide-free-ab305240--immunocytochemistry-immunofluorescence-img403902.jpg "Immunocytochemistry/ Immunofluorescence - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240)")
Immunocytochemistry/ Immunofluorescence - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240)
This data was developed using Anti-UBR7 antibody [EPR26170-67] ab305239, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling UBR7 with Anti-UBR7 antibody [EPR26170-67] ab305239 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in NIH/3T3 cell line.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red).
The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240), expandable thumbnail](https://content.abcam.com/products/images/ubr7-antibody-epr26170-67-bsa-and-azide-free-ab305240--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403827.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240)
This data was developed using Anti-UBR7 antibody [EPR26170-67] ab305239, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling UBR7 with Anti-UBR7 antibody [EPR26170-67] ab305239 at 1/500 (0.942 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining in rat colon. The section was incubated with Anti-UBR7 antibody [EPR26170-67] ab305239 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240), expandable thumbnail](https://content.abcam.com/products/images/ubr7-antibody-epr26170-67-bsa-and-azide-free-ab305240--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403824.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240), expandable thumbnail](https://content.abcam.com/products/images/ubr7-antibody-epr26170-67-bsa-and-azide-free-ab305240--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403825.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240), expandable thumbnail](https://content.abcam.com/products/images/ubr7-antibody-epr26170-67-bsa-and-azide-free-ab305240--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403826.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240)
![Immunoprecipitation - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240), expandable thumbnail](https://content.abcam.com/products/images/ubr7-antibody-epr26170-67-bsa-and-azide-free-ab305240--immunoprecipitation-img404422.jpg "Immunoprecipitation - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240)")
Immunoprecipitation - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240)
This data was developed using Anti-UBR7 antibody [EPR26170-67] ab305239, the same antibody clone in a different buffer formulation.
UBR7 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast), whole cell lysate 10 μg with Anti-UBR7 antibody [EPR26170-67] ab305239 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-UBR7 antibody [EPR26170-67] ab305239 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate 10 μg
Lane 2: Anti-UBR7 antibody [EPR26170-67] ab305239 IP in NIH/3T3 whole cell lysate
Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-UBR7 antibody [EPR26170-67] ab305239 in NIH/3T3 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 24 seconds
The bands beneath the target band (55 kDa) are likely to be degraded target fragmentsAll lanes: Immunoprecipitation - Anti-UBR7 antibody [EPR26170-67] (Anti-UBR7 antibody [EPR26170-67] ab305239) at 1/30 dilution
All lanes: NIH/3T3 (mouse embryonic fibroblast)
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 55 kDa
Exposure time: 24s
UBR7 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast), whole cell lysate 10 μg with Anti-UBR7 antibody [EPR26170-67] ab305239 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-UBR7 antibody [EPR26170-67] ab305239 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate 10 μg
Lane 2: Anti-UBR7 antibody [EPR26170-67] ab305239 IP in NIH/3T3 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-UBR7 antibody [EPR26170-67] ab305239 in NIH/3T3 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 24 seconds
The bands beneath the target band (55 kDa) are likely to be degraded target fragments![Immunoprecipitation - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240), expandable thumbnail](https://content.abcam.com/products/images/ubr7-antibody-epr26170-67-bsa-and-azide-free-ab305240--immunoprecipitation-img404421.jpg "Immunoprecipitation - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240)")
Immunoprecipitation - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240)
This data was developed using Anti-UBR7 antibody [EPR26170-67] ab305239, the same antibody clone in a different buffer formulation.
UBR7 was immunoprecipitated from 0.35 mg MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate 10 μg
with Anti-UBR7 antibody [EPR26170-67] ab305239 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-UBR7 antibody [EPR26170-67] ab305239 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate 10 μg
Lane 2: Anti-UBR7 antibody [EPR26170-67] ab305239 IP in MCF7 whole cell lysate
Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-UBR7 antibody [EPR26170-67] ab305239 in MCF7 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 seconds
The bands beneath the target band (55 kDa) are likely to be degraded target fragmentsAll lanes: Immunoprecipitation - Anti-UBR7 antibody [EPR26170-67] (Anti-UBR7 antibody [EPR26170-67] ab305239) at 1/30 dilution
All lanes: MCF7 (human breast adenocarcinoma epithelial cell)
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 55 kDa
Exposure time: 32s
UBR7 was immunoprecipitated from 0.35 mg MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate 10 μg
with Anti-UBR7 antibody [EPR26170-67] ab305239 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-UBR7 antibody [EPR26170-67] ab305239 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate 10 μg
Lane 2: Anti-UBR7 antibody [EPR26170-67] ab305239 IP in MCF7 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-UBR7 antibody [EPR26170-67] ab305239 in MCF7 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 seconds
The bands beneath the target band (55 kDa) are likely to be degraded target fragments![Western blot - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240), expandable thumbnail](https://content.abcam.com/products/images/ubr7-antibody-epr26170-67-bsa-and-azide-free-ab305240--western-blot-img404143.jpg "Western blot - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240)")
Western blot - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240)
This data was developed using Anti-UBR7 antibody [EPR26170-67] ab305239, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The bands beneath the target band (55 kDa) are likely to be degraded target fragments
Exposure time: 3 minutesAll lanes: Western blot - Anti-UBR7 antibody [EPR26170-67] (Anti-UBR7 antibody [EPR26170-67] ab305239) at 1/1000 dilution
Lane 1: T-47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate at 20 µg
Lane 2: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg
Lane 3: C6 (rat glial tumor glial cell), whole cell lysate at 20 µg
Lane 4: Human testis tissue lysate at 20 µg
Lane 5: Mouse testis tissue lysate at 20 µg
Lane 6: Rat testis tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 55 kDa
Exposure time: 3min
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The bands beneath the target band (55 kDa) are likely to be degraded target fragments
Exposure time: 3 minutes![Western blot - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240), expandable thumbnail](https://content.abcam.com/products/images/ubr7-antibody-epr26170-67-bsa-and-azide-free-ab305240--western-blot-img404142.jpg "Western blot - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240)")
Western blot - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240)
This data was developed using Anti-UBR7 antibody [EPR26170-67] ab305239, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 48 secondsAll lanes: Western blot - Anti-UBR7 antibody [EPR26170-67] (Anti-UBR7 antibody [EPR26170-67] ab305239) at 1/1000 dilution
Lane 1: MCF7 (human breast adenocarcinoma epithelial cell)transfected with scrambled siRNA control, whole cell lysate at 20 µg
Lane 2: MCF7 transfected with siRNA specifically targeti UBR7, whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 55 kDa
Exposure time: 48s
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 48 seconds![Western blot - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240), expandable thumbnail](https://content.abcam.com/products/images/ubr7-antibody-epr26170-67-bsa-and-azide-free-ab305240--western-blot-img404141.jpg "Western blot - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240)")
Western blot - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240)
This data was developed using Anti-UBR7 antibody [EPR26170-67] ab305239, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
Exposure time: 3 minutesAll lanes: Western blot - Anti-UBR7 antibody [EPR26170-67] (Anti-UBR7 antibody [EPR26170-67] ab305239) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2: 293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg
Lane 3: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg
Lane 4: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg
Lane 5: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 55 kDa
Exposure time: 3min
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
Exposure time: 3 minutes![Flow Cytometry (Intracellular) - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240), expandable thumbnail](https://content.abcam.com/products/images/ubr7-antibody-epr26170-67-bsa-and-azide-free-ab305240--flow-cytometry-intracellular-img403943.jpg "Flow Cytometry (Intracellular) - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240)")
Flow Cytometry (Intracellular) - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240)
This data was developed using Anti-UBR7 antibody [EPR26170-67] ab305239, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling UBR7 with Anti-UBR7 antibody [EPR26170-67] ab305239 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.![Flow Cytometry (Intracellular) - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240), expandable thumbnail](https://content.abcam.com/products/images/ubr7-antibody-epr26170-67-bsa-and-azide-free-ab305240--flow-cytometry-intracellular-img403942.jpg "Flow Cytometry (Intracellular) - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240)")
Flow Cytometry (Intracellular) - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240)
This data was developed using Anti-UBR7 antibody [EPR26170-67] ab305239, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (human breast adenocarcinoma epithelial cell) cells labelling UBR7 with Anti-UBR7 antibody [EPR26170-67] ab305239 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.![Immunocytochemistry - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240), expandable thumbnail](https://content.abcam.com/products/images/ubr7-antibody-epr26170-67-bsa-and-azide-free-ab305240--immunocytochemistry-img403901.jpg "Immunocytochemistry - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240)")
Immunocytochemistry - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240)
This data was developed using Anti-UBR7 antibody [EPR26170-67] ab305239, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MCF7 (human breast adenocarcinoma epithelial cell) cells labelling UBR7 with Anti-UBR7 antibody [EPR26170-67] ab305239 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in MCF7 cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red).
The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
![Immunoprecipitation - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240), expandable thumbnail](https://content.abcam.com/products/images/ubr7-antibody-epr26170-67-bsa-and-azide-free-ab305240--immunoprecipitation-img404422.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240)")
![Immunoprecipitation - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240), expandable thumbnail](https://content.abcam.com/products/images/ubr7-antibody-epr26170-67-bsa-and-azide-free-ab305240--immunoprecipitation-img404421.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240)")
![Western blot - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240), expandable thumbnail](https://content.abcam.com/products/images/ubr7-antibody-epr26170-67-bsa-and-azide-free-ab305240--western-blot-img404143.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240)")
![Western blot - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240), expandable thumbnail](https://content.abcam.com/products/images/ubr7-antibody-epr26170-67-bsa-and-azide-free-ab305240--western-blot-img404142.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240)")
![Western blot - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240), expandable thumbnail](https://content.abcam.com/products/images/ubr7-antibody-epr26170-67-bsa-and-azide-free-ab305240--western-blot-img404141.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240)")
![Flow Cytometry (Intracellular) - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240), expandable thumbnail](https://content.abcam.com/products/images/ubr7-antibody-epr26170-67-bsa-and-azide-free-ab305240--flow-cytometry-intracellular-img403943.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240)")
![Flow Cytometry (Intracellular) - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240), expandable thumbnail](https://content.abcam.com/products/images/ubr7-antibody-epr26170-67-bsa-and-azide-free-ab305240--flow-cytometry-intracellular-img403942.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240)")
![Immunocytochemistry - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240), expandable thumbnail](https://content.abcam.com/products/images/ubr7-antibody-epr26170-67-bsa-and-azide-free-ab305240--immunocytochemistry-img403901.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-UBR7 antibody [EPR26170-67] - BSA and Azide free (ab305240)")
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