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AB154064

Anti-UBXD8 antibody

4

(1 Review)

|

(2 Publications)

Rabbit Polyclonal UBXD8 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FAF2 aa 150-400.

View Alternative Names

ETEA, KIAA0887, UBXD8, UBXN3B, FAF2, FAS-associated factor 2, UBX domain-containing protein 3B, UBX domain-containing protein 8

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXD8 antibody (AB154064)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXD8 antibody (AB154064)

Immunohistochemical analysis of paraffin embedded Hs578T xenograft tissue, labeling UBXD8 using ab154064 at 1/100 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-UBXD8 antibody (AB154064)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-UBXD8 antibody (AB154064)

Immunofluorescence analysis of methanol fixed A431 cells, labeling UBXD8 using ab154064 at 1/200 dilution. Lower image costained with Hoerchst 33342.

Western blot - Anti-UBXD8 antibody (AB154064)
  • WB

Unknown

Western blot - Anti-UBXD8 antibody (AB154064)

10% SDS PAGE

All lanes:

Western blot - Anti-UBXD8 antibody (ab154064) at 1/1000 dilution

All lanes:

293T whole cell lysate at 30 µg

Predicted band size: 53 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human FAF2 aa 150-400. The exact immunogen used to generate this antibody is proprietary information.

Q96CS3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UBXD8 also known as UBX domain-containing protein 8 or FAF2 is an essential player in cellular regulation. This protein weighs approximately 64 kDa and is expressed in various tissues with prominent activity in the liver and adipose tissue. Mechanically UBXD8 functions as part of the Endoplasmic Reticulum-Associated Degradation (ERAD) pathway where it interacts with other proteins to facilitate the extraction and turnover of misfolded proteins. UBXD8 being part of the p97/VCP protein complex plays an important role in maintaining protein homeostasis within cells.
Biological function summary

UBXD8 regulates lipid metabolism and transport. It ensures lipid droplets' stability and controls lipid efflux from the liver contributing to systemic lipid homeostasis. The protein acts as a bridge in the process of tethering lipid droplets to the ER membrane thereby participating in lipid droplet biogenesis and turnover. As part of a larger complex with p97/VCP UBXD8 supervises the transfer and processing of lipid-related components linking it dynamically to cellular lipid management.

Pathways

The biogenesis of lipid droplets and the pathway of protein degradation heavily involve UBXD8. It interacts with pathways such as the unfolded protein response (UPR) ensuring the ER handles protein misfolding effectively. Collaborating closely with proteins like p97/VCP and E4B UBXD8 supports the ubiquitin-proteasome system which leads to efficient degradation of defective proteins. These interactions highlight UBXD8’s participation in cellular stress responses and maintenance of cellular equilibrium.

Aberrations in UBXD8 function link it to metabolic and neurodegenerative diseases. In conditions like non-alcoholic fatty liver disease (NAFLD) UBXD8's dysfunction can disrupt normal lipid storage and cause excessive fat accumulation in the liver. Also improper activity connects UBXD8 to neurodegenerative disorders such as Alzheimer's disease where it might influence protein aggregation and clearance. Here its interaction with p97/VCP becomes significant suggesting potential therapeutic targets in correcting or mitigating these pathological conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays an important role in endoplasmic reticulum-associated degradation (ERAD) that mediates ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins (PubMed : 18711132, PubMed : 24215460). By controlling the steady-state expression of the IGF1R receptor, indirectly regulates the insulin-like growth factor receptor signaling pathway (PubMed : 26692333). Involved in inhibition of lipid droplet degradation by binding to phospholipase PNPL2 and inhibiting its activity by promoting dissociation of PNPL2 from its endogenous activator, ABHD5 which inhibits the rate of triacylglycerol hydrolysis (PubMed : 23297223). Involved in stress granule disassembly : associates with ubiquitinated G3BP1 in response to heat shock, thereby promoting interaction between ubiquitinated G3BP1 and VCP, followed by G3BP1 extraction from stress granules and stress granule disassembly (PubMed : 34739333).
See full target information FAF2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 41:e110777 PubMed35993436

2022

Regulation of membrane fluidity by RNF145-triggered degradation of the lipid hydrolase ADIPOR2.

Applications

Unspecified application

Species

Unspecified reactive species

Norbert Volkmar,Christian M Gawden-Bone,James C Williamson,Jonathon Nixon-Abell,James A West,Peter H St George-Hyslop,Arthur Kaser,Paul J Lehner

PLoS pathogens 13:e1006674 PubMed28985237

2017

Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Wang,Bei Wang,He Huang,Chongyang Zhang,Yuanmei Zhu,Bin Pei,Chaofei Cheng,Lei Sun,Jianwei Wang,Qi Jin,Zhendong Zhao
View all publications
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