AB96673

Anti-UBXD8 antibody

Name: Anti-UBXD8 antibody (ab96673) | Abcam
Brand: Abcam Limited
SKU: AB96673

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(1 Publication)

Rabbit Polyclonal UBXD8 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human FAF2 aa 350 to C-terminus.

View Alternative Names

ETEA, KIAA0887, UBXD8, UBXN3B, FAF2, FAS-associated factor 2, UBX domain-containing protein 3B, UBX domain-containing protein 8

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-UBXD8 antibody (AB96673)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-UBXD8 antibody (AB96673)

4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling UBXD8 using ab96673 at 1/500 dilution (green) in ICC/IF. The nuclear counter stain is Hoechst 33343 (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXD8 antibody (AB96673)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXD8 antibody (AB96673)

Paraffin-embedded mouse intestine tissue labeling UBXD8 using ab96673 at1/500 dilution in immunohistochemical analysis.

Antigen retrieval : EDTA-based (pH8.0), 15 minutes.

Unknown

Western blot - Anti-UBXD8 antibody (AB96673)

10% SDS PAGE

All lanes:

Western blot - Anti-UBXD8 antibody (ab96673) at 1/1000 dilution

Lane 1:

H1299 whole cell lysate at 30 µg

Lane 2:

HeLa whole cell lysate at 30 µg

Predicted band size: 53 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human FAF2 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96CS3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UBXD8 also known as UBX domain-containing protein 8 or FAF2 is an essential player in cellular regulation. This protein weighs approximately 64 kDa and is expressed in various tissues with prominent activity in the liver and adipose tissue. Mechanically UBXD8 functions as part of the Endoplasmic Reticulum-Associated Degradation (ERAD) pathway where it interacts with other proteins to facilitate the extraction and turnover of misfolded proteins. UBXD8 being part of the p97/VCP protein complex plays an important role in maintaining protein homeostasis within cells.

Biological function summary

UBXD8 regulates lipid metabolism and transport. It ensures lipid droplets' stability and controls lipid efflux from the liver contributing to systemic lipid homeostasis. The protein acts as a bridge in the process of tethering lipid droplets to the ER membrane thereby participating in lipid droplet biogenesis and turnover. As part of a larger complex with p97/VCP UBXD8 supervises the transfer and processing of lipid-related components linking it dynamically to cellular lipid management.

Pathways

The biogenesis of lipid droplets and the pathway of protein degradation heavily involve UBXD8. It interacts with pathways such as the unfolded protein response (UPR) ensuring the ER handles protein misfolding effectively. Collaborating closely with proteins like p97/VCP and E4B UBXD8 supports the ubiquitin-proteasome system which leads to efficient degradation of defective proteins. These interactions highlight UBXD8’s participation in cellular stress responses and maintenance of cellular equilibrium.

Aberrations in UBXD8 function link it to metabolic and neurodegenerative diseases. In conditions like non-alcoholic fatty liver disease (NAFLD) UBXD8's dysfunction can disrupt normal lipid storage and cause excessive fat accumulation in the liver. Also improper activity connects UBXD8 to neurodegenerative disorders such as Alzheimer's disease where it might influence protein aggregation and clearance. Here its interaction with p97/VCP becomes significant suggesting potential therapeutic targets in correcting or mitigating these pathological conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays an important role in endoplasmic reticulum-associated degradation (ERAD) that mediates ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins (PubMed : 18711132, PubMed : 24215460). By controlling the steady-state expression of the IGF1R receptor, indirectly regulates the insulin-like growth factor receptor signaling pathway (PubMed : 26692333). Involved in inhibition of lipid droplet degradation by binding to phospholipase PNPL2 and inhibiting its activity by promoting dissociation of PNPL2 from its endogenous activator, ABHD5 which inhibits the rate of triacylglycerol hydrolysis (PubMed : 23297223). Involved in stress granule disassembly : associates with ubiquitinated G3BP1 in response to heat shock, thereby promoting interaction between ubiquitinated G3BP1 and VCP, followed by G3BP1 extraction from stress granules and stress granule disassembly (PubMed : 34739333).

See full target information FAF2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Human mutation 42:150-163 PubMed33169439

2020

The p.Ser64Leu and p.Pro104Leu missense variants of PALB2 identified in familial pancreatic cancer patients compromise the DNA damage response.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Zhang,Jung-Young Park,Fan Zhang,Sara H Olson,Irene Orlow,Yirong Li,Robert C Kurtz,Marc Ladanyi,Jie Chen,Amanda E Toland,Liying Zhang,Paul R Andreassen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-UBXD8 antibody

Immunocytochemistry/ Immunofluorescence - Anti-UBXD8 antibody (AB96673)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXD8 antibody (AB96673)

Western blot - Anti-UBXD8 antibody (AB96673)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Proteins & Peptides

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise