AB151723

Anti-UBXN1 antibody [EPR9136]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal UBXN1 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

SAKS1, UBXN1, UBX domain-containing protein 1, SAPK substrate protein 1, UBA/UBX 33.3 kDa protein

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (AB151723)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (AB151723)

Immunohistochemical analysis of paraffin embedded normal Human uterus tissue using ab151723 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (AB151723)

Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab151723 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (AB151723)

Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling UBXN1 with ab151723 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-UBXN1 antibody [EPR9136] (AB151723)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-UBXN1 antibody [EPR9136] (AB151723)

Intracellular Flow Cytometry analysis of permeabilized 293T cells labeling UBXN1 with ab151723 at 1/10 dilution (red) compared to a nonspecific control antibody (green).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (AB151723)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling UBXN1 with ab151723 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (AB151723)

Immunohistochemical analysis of paraffin embedded Human Lung carcinoma tissue using ab151723 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (AB151723)

Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab151723 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-UBXN1 antibody [EPR9136] (AB151723)

All lanes:

Western blot - Anti-UBXN1 antibody [EPR9136] (ab151723) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

Ramos cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 33 kDa

false

Carrier free

Anti-UBXN1 antibody [EPR9136] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9136

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UBXN1 also known as UBX domain-containing protein 1 has a major role in regulating protein degradation through the ubiquitin-proteasome system. This protein weighs approximately 38 kDa. UBXN1 is expressed ubiquitously with higher levels reported in tissues such as kidney liver and brain. It interacts with the p97/VCP ATPase modulating its activity in protein degradation. By binding to ubiquitin chains UBXN1 influences the fate of many substrates targeted for degradation.

Biological function summary

UBXN1 serves as a regulator in processes such as protein homeostasis and stress response. It functions as part of a large protein complex that includes elements like p97/VCP which are central to maintaining cellular protein balance. UBXN1 modulates processes involving endoplasmic reticulum-associated degradation (ERAD). It therefore plays a role in the quality control of misfolded proteins preventing their accumulation and potential toxicity in cells.

Pathways

UBXN1 is closely associated with the ubiquitin-proteasome pathway a critical system for protein turnover. It acts along with proteins such as p97/VCP and works in tandem with ubiquitin enzymes to regulate protein substrates. Additionally UBXN1 has a role in autophagy-related pathways where it assists in removing damaged proteins and organelles cooperating with signalling proteins like LC3 which are essential for autophagic vesicle formation.

UBXN1 has connections with neurodegenerative diseases and cancer. Its involvement in protein homeostasis and degradation pathways links UBXN1 to neurodegenerative conditions where mismanagement of protein quality control is a hallmark. The dysregulation of UBXN1 alongside proteins p97/VCP has been associated with inclusion body myopathy and other degenerative diseases. Moreover alterations in UBXN1 expression or function relate to cancer progression as its imbalance can lead to uncontrolled cell growth through disrupted protein degradation mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ubiquitin-binding protein that plays a role in the modulation of innate immune response. Blocks both the RIG-I-like receptors (RLR) and NF-kappa-B pathways. Following viral infection, UBXN1 is induced and recruited to the RLR component MAVS. In turn, interferes with MAVS oligomerization, and disrupts the MAVS/TRAF3/TRAF6 signalosome. This function probably serves as a brake to prevent excessive RLR signaling (PubMed : 23545497). Interferes with the TNFalpha-triggered NF-kappa-B pathway by interacting with cellular inhibitors of apoptosis proteins (cIAPs) and thereby inhibiting their recruitment to TNFR1 (PubMed : 25681446). Prevents also the activation of NF-kappa-B by associating with CUL1 and thus inhibiting NF-kappa-B inhibitor alpha/NFKBIA degradation that remains bound to NF-kappa-B (PubMed : 28152074). Interacts with the BRCA1-BARD1 heterodimer and regulates its activity. Specifically binds 'Lys-6'-linked polyubiquitin chains. Interaction with autoubiquitinated BRCA1 leads to the inhibition of the E3 ubiquitin-protein ligase activity of the BRCA1-BARD1 heterodimer (PubMed : 20351172). Component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulum that are retrotranslocated in the cytosol.

See full target information UBXN1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Aging cell 24:e70018 PubMed39957408

2025

Activated mTOR Signaling in the RPE Drives EMT, Autophagy, and Metabolic Disruption, Resulting in AMD-Like Pathology in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Olivia Chowdhury,Sridhar Bammidi,Pooja Gautam,Vishnu Suresh Babu,Haitao Liu,Peng Shang,Ying Xin,Emma Mahally,Mihir Nemani,Victoria Koontz,Kira Lathrop,Katarzyna M Kedziora,Jonathan Franks,Ming Sun,Joshua W Smith,Lauren R DeVine,Robert N Cole,Nadezda Stepicheva,Anastasia Strizhakova,Sreya Chattopadhyay,Stacey Hose,Jacob Samuel Zigler,José-Alain Sahel,Jiang Qian,Prasun Guha,James T Handa,Sayan Ghosh,Debasish Sinha

Autophagy 18:2615-2635 PubMed35253629

2022

Therapeutic targeting of the USP2-E2F4 axis inhibits autophagic machinery essential for zinc homeostasis in cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing Xiao,Jianqun Wang,Xiaojing Wang,Shuang Cai,Yanhua Guo,Lin Ye,Dan Li,Anpei Hu,Shikai Jin,Boling Yuan,Yi Zhou,Qilan Li,Qiangsong Tong,Liduan Zheng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-UBXN1 antibody [EPR9136]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (AB151723)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (AB151723)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (AB151723)

Flow Cytometry (Intracellular) - Anti-UBXN1 antibody [EPR9136] (AB151723)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (AB151723)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (AB151723)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBXN1 antibody [EPR9136] (AB151723)

Western blot - Anti-UBXN1 antibody [EPR9136] (AB151723)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise