Rabbit Polyclonal UBXN6/UBXD1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human UBXN6 aa 1-100.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human UBXN6 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BZV1
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Target data
Function
May negatively regulate the ATPase activity of VCP, an ATP-driven segregase that associates with different cofactors to control a wide variety of cellular processes (PubMed:26475856). As a cofactor of VCP, it may play a role in the transport of CAV1 to lysosomes for degradation (PubMed:21822278, PubMed:23335559). It may also play a role in endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins (PubMed:19275885). Together with VCP and other cofactors, it may play a role in macroautophagy, regulating for instance the clearance of damaged lysosomes (PubMed:27753622).
Alternative names
UBXD1, UBXDC2, UBXN6, UBX domain-containing protein 6, UBX domain-containing protein 1
Recommended products
Rabbit Polyclonal UBXN6/UBXD1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human UBXN6 aa 1-100.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human UBXN6 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BZV1
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
UBXN6 also known as UBXD1 is a protein with a molecular mass of approximately 48 kDa. This protein is expressed across various tissues showing higher levels in skeletal muscle heart and kidney. UBXN6 contains a UBX domain which is essential for its interactions with other proteins especially those in the ubiquitin-proteasome system. Mechanically UBXN6 acts as a cofactor modulating the functions of several cellular machineries involved in protein degradation.
- Biological function summary
Four words describe UBXN6's relevance in cellular homeostasis. UBXN6 plays an important role in the regulation of protein turnover as it is involved in the ubiquitin-proteasome complex. It assists in the disassembly of protein substrates tagged for degradation contributing to the maintenance of protein quality control within the cell. Through interaction with p97/VCP UBXN6 helps in processes like endoplasmic reticulum-associated degradation (ERAD) important for cellular response to misfolded proteins.
- Pathways
UBXN6's involvement extends to the autophagy and ubiquitin-proteasome pathways. In the ubiquitin-proteasome pathway it acts as a regulatory component favoring the removal of damaged or unnecessary proteins by acting with partners like p97/VCP. Through the autophagy pathway UBXN6 aids in cellular cleanup and recycling processes supporting cellular survival mechanisms during stress conditions. These pathways highlight UBXN6's integral role in protein quality control and cellular defense.
- Associated diseases and disorders
Four words explain UBXN6's clinical importance. Research links UBXN6 to neurological disorders such as amyotrophic lateral sclerosis (ALS) where misfolded proteins contribute to disease progress. Moreover UBXN6 relates to cancer especially in tumor progression where impaired protein degradation can lead to uncontrolled cell growth. In both cases UBXN6 interacts closely with p97/VCP offering potential therapeutic targets for drug development aimed at modulating its activity to restore cellular balance.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-UBXN6/UBXD1 antibody (ab221167)
Immunofluorescent analysis of PFA-fixed/Triton X-100 permeabilized HEK293 cells labeling UBXN6/UBXD1 with ab221167 at 4 μg/ml (green).
Western blot - Anti-UBXN6/UBXD1 antibody (ab221167)
All lanes: Western blot - Anti-UBXN6/UBXD1 antibody (ab221167) at 1/500 dilution
Lane 1: RT4 cell lysate
Lane 2: U251 MG cell lysate
Predicted band size: 49 kDa
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