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AB221167

Anti-UBXN6/UBXD1 antibody

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(2 Publications)

Rabbit Polyclonal UBXN6/UBXD1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human UBXN6 aa 1-100.

View Alternative Names

UBXD1, UBXDC2, UBXN6, UBX domain-containing protein 6, UBX domain-containing protein 1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-UBXN6/UBXD1 antibody (AB221167)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-UBXN6/UBXD1 antibody (AB221167)

Immunofluorescent analysis of PFA-fixed/Triton X-100 permeabilized HEK293 cells labeling UBXN6/UBXD1 with ab221167 at 4 μg/ml (green).

Western blot - Anti-UBXN6/UBXD1 antibody (AB221167)
  • WB

Supplier Data

Western blot - Anti-UBXN6/UBXD1 antibody (AB221167)

All lanes:

Western blot - Anti-UBXN6/UBXD1 antibody (ab221167) at 1/500 dilution

Lane 1:

RT4 cell lysate

Lane 2:

U251 MG cell lysate

Predicted band size: 49 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human UBXN6 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q9BZV1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UBXN6 also known as UBXD1 is a protein with a molecular mass of approximately 48 kDa. This protein is expressed across various tissues showing higher levels in skeletal muscle heart and kidney. UBXN6 contains a UBX domain which is essential for its interactions with other proteins especially those in the ubiquitin-proteasome system. Mechanically UBXN6 acts as a cofactor modulating the functions of several cellular machineries involved in protein degradation.
Biological function summary

UBXN6 plays an important role in the regulation of protein turnover as it is involved in the ubiquitin-proteasome complex. It assists in the disassembly of protein substrates tagged for degradation contributing to the maintenance of protein quality control within the cell. Through interaction with p97/VCP UBXN6 helps in processes like endoplasmic reticulum-associated degradation (ERAD) important for cellular response to misfolded proteins.

Pathways

UBXN6's involvement extends to the autophagy and ubiquitin-proteasome pathways. In the ubiquitin-proteasome pathway it acts as a regulatory component favoring the removal of damaged or unnecessary proteins by acting with partners like p97/VCP. Through the autophagy pathway UBXN6 aids in cellular cleanup and recycling processes supporting cellular survival mechanisms during stress conditions. These pathways highlight UBXN6's integral role in protein quality control and cellular defense.

Research links UBXN6 to neurological disorders such as amyotrophic lateral sclerosis (ALS) where misfolded proteins contribute to disease progress. Moreover UBXN6 relates to cancer especially in tumor progression where impaired protein degradation can lead to uncontrolled cell growth. In both cases UBXN6 interacts closely with p97/VCP offering potential therapeutic targets for drug development aimed at modulating its activity to restore cellular balance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May negatively regulate the ATPase activity of VCP, an ATP-driven segregase that associates with different cofactors to control a wide variety of cellular processes (PubMed : 26475856). As a cofactor of VCP, it may play a role in the transport of CAV1 to lysosomes for degradation (PubMed : 21822278, PubMed : 23335559). It may also play a role in endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins (PubMed : 19275885). Together with VCP and other cofactors, it may play a role in macroautophagy, regulating for instance the clearance of damaged lysosomes (PubMed : 27753622).
See full target information UBXN6
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Signal transduction and targeted therapy 10:192 PubMed40514371

2025

Stem loop binding protein promotes SARS-CoV-2 replication via -1 programmed ribosomal frameshifting.

Applications

Unspecified application

Species

Unspecified reactive species

Tanxiu Chen,Ruimin Zhu,Tingfu Du,Hao Yang,Xintian Zhang,Zhixing Wang,Yong Zhang,Wenqi Quan,Bin Yin,Yunpeng Liu,Shuaiyao Lu,Xiaozhong Peng

The Journal of cell biology 222: PubMed36880596

2023

The p97/VCP segregase is essential for arsenic-induced degradation of PML and PML-RARA.

Applications

Unspecified application

Species

Unspecified reactive species

Ellis G Jaffray,Michael H Tatham,Barbara Mojsa,Magda Liczmanska,Alejandro Rojas-Fernandez,Yili Yin,Graeme Ball,Ronald T Hay
View all publications
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