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AB124931

Anti-UCH37 antibody [EPR4896]

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(14 Publications)

Rabbit Recombinant Monoclonal UCH37 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 14 publications.

View Alternative Names

UCH37, AD-019, CGI-70, UCHL5, Ubiquitin carboxyl-terminal hydrolase isozyme L5, UCH-L5, Ubiquitin C-terminal hydrolase UCH37, Ubiquitin thioesterase L5

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCH37 antibody [EPR4896] (AB124931)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCH37 antibody [EPR4896] (AB124931)

ab124931 at 1/500 dilution staining UCH37 in paraffin embedded Human kidney tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-UCH37 antibody [EPR4896] (AB124931)
  • WB

Unknown

Western blot - Anti-UCH37 antibody [EPR4896] (AB124931)

All lanes:

Western blot - Anti-UCH37 antibody [EPR4896] (ab124931) at 1/1000 dilution

Lane 1:

Human fetal kidney cell lysates at 10 µg

Lane 2:

293T cell lysates at 10 µg

Lane 3:

NCCIT cell lysates at 10 µg

Lane 4:

SH-SY5Y cell lysates at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 38 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4896

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p>Heat up to 98°C, below boiling, and then let cool for 10-20 min.</p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p>Heat up to 98°C, below boiling, and then let cool for 10-20 min.</p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UCH37 also known as ubiquitin carboxyl-terminal hydrolase L5 (UCHL5) is a deubiquitinating enzyme of approximately 37 kDa mass. This protein functions by cleaving ubiquitin moieties from polyubiquitin chains facilitating protein degradation or recycling processes. UCH37 expression is observed in various tissues with higher expression noted in the liver and kidney indicating its significant cellular roles in these organs.
Biological function summary

UCH37 plays a role in the ubiquitin-proteasome system where it is an integral component of the 19S regulatory particle of the 26S proteasome complex. Through this association UCH37 participates in the removal of ubiquitin from substrates targeted for proteasomal degradation. This action regulates protein turnover and prevents unnecessary degradation of proteins which is important for maintaining cell homeostasis and controlling protein quality.

Pathways

The modulation of proteostasis by UCH37 directly influences the ubiquitin-proteasome pathway and the associated cellular stress responses. Its activity can impact the NF-kappaB signaling pathway by regulating the stability of components within this pathway interacting with other proteins such as the proteasome subunit RPN13 and proteasome-associated DUBs. The precise regulation of protein levels within these pathways ensures that cells respond appropriately to stress and inflammation.

Abnormal regulation or expression of UCH37 contributes to several pathological conditions. Cancer and neurodegenerative disorders are two prominent examples in which UCH37 plays a role. Elevated levels or dysfunctional UCH37 activity can disrupt proteostasis leading to uncontrolled cell proliferation or cell death resistance in cancer or the accumulation of misfolded proteins in conditions like Alzheimer’s disease. Additionally interactions of UCH37 with oncogenic proteins or tau proteins in neurodegenerative conditions reflect its relevance in the progression of these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1.
See full target information UCHL5
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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Autophagy 20:1314-1334 PubMed38174993

2024

Targeting cancer-associated fibroblast autophagy renders pancreatic cancer eradicable with immunochemotherapy by inhibiting adaptive immune resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaozhen Zhang,Mengyi Lao,Hanshen Yang,Kang Sun,Yunfei Dong,Lihong He,Xinchi Jiang,Honghui Wu,Yangwei Jiang,Muchun Li,Honggang Ying,Xinyuan Liu,Jian Xu,Yan Chen,Hanjia Zhang,Ruhong Zhou,Jianqing Gao,Xueli Bai,Tingbo Liang

Frontiers in molecular biosciences 9:866467 PubMed35720124

2022

Inhibiting UCH-L5: Rational Design of a Cyclic Ubiquitin-Based Peptide Inhibitor.

Applications

Unspecified application

Species

Unspecified reactive species

Dharjath S Hameed,Huib Ovaa,Gerbrand J van der Heden van Noort,Aysegul Sapmaz

eLife 11: PubMed35451368

2022

A cryptic K48 ubiquitin chain binding site on UCH37 is required for its role in proteasomal degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Jiale Du,Sandor Babik,Yanfeng Li,Kirandeep K Deol,Stephen J Eyles,Jasna Fejzo,Marco Tonelli,Eric Strieter

Nature communications 12:4608 PubMed34326324

2021

Antibody toolkit reveals N-terminally ubiquitinated substrates of UBE2W.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher W Davies,Simon E Vidal,Lilian Phu,Jawahar Sudhamsu,Trent B Hinkle,Scott Chan Rosenberg,Frances-Rose Schumacher,Yi Jimmy Zeng,Carsten Schwerdtfeger,Andrew S Peterson,Jennie R Lill,Christopher M Rose,Andrey S Shaw,Ingrid E Wertz,Donald S Kirkpatrick,James T Koerber

Clinical and translational medicine 10:e241 PubMed33377647

2020

MAFG-AS1 promotes tumor progression via regulation of the HuR/PTBP1 axis in bladder urothelial carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Mengqing Xiao,Jianye Liu,Liang Xiang,Kai Zhao,Dong He,Qinghai Zeng,Qun Zhang,Dan Xie,Minhua Deng,Yuxing Zhu,Yeyu Zhang,Yan Liu,Hao Bo,Xiaoming Liu,Xingyu Chen,Lian Gong,Ying Bao,Yi Hu,Yaxin Cheng,Liping Deng,Rongrong Zhu,Xiaowei Xing,Ming Zhou,Wei Xiong,Yanhong Zhou,Jianda Zhou,Xiaohui Li,Ke Cao

Molecular cell 80:796-809.e9 PubMed33156996

2020

Proteasome-Bound UCH37/UCHL5 Debranches Ubiquitin Chains to Promote Degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Kirandeep K Deol,Sean O Crowe,Jiale Du,Heather A Bisbee,Robert G Guenette,Eric R Strieter

Journal of experimental & clinical cancer research 38:453 PubMed31694672

2019

Proteasomal cysteine deubiquitinase inhibitor b-AP15 suppresses migration and induces apoptosis in diffuse large B cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Liling Jiang,Yuening Sun,Jinxiang Wang,Qingyan He,Xinmei Chen,Xiaoying Lan,Jinghong Chen,Q Ping Dou,Xianping Shi,Jinbao Liu

Apoptosis : an international journal on programmed cell death 24:826-836 PubMed31342239

2019

Deubiquitylatinase inhibitor b-AP15 induces c-Myc-Noxa-mediated apoptosis in esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Beibei Sha,Xiaoyu Chen,Han Wu,Miaomiao Li,Jianxiang Shi,Longhao Wang,Xingge Liu,Ping Chen,Tao Hu,Pei Li

Frontiers in physiology 10:534 PubMed31130875

2019

Detailed Dissection of UBE3A-Mediated DDI1 Ubiquitination.

Applications

Unspecified application

Species

Unspecified reactive species

Nagore Elu,Nerea Osinalde,Javier Beaskoetxea,Juanma Ramirez,Benoit Lectez,Kerman Aloria,Jose Antonio Rodriguez,Jesus M Arizmendi,Ugo Mayor

Cell discovery 5:24 PubMed31044085

2019

USP10 modulates the SKP2/Bcr-Abl axis via stabilizing SKP2 in chronic myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Yuning Liao,Ningning Liu,Xiaohong Xia,Zhiqiang Guo,Yanling Li,Lili Jiang,Ruiqing Zhou,Daolin Tang,Hongbiao Huang,Jinbao Liu
View all publications
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Product promise

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