Skip to main content

Rabbit Recombinant Monoclonal UCHL3 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-UCHL3 antibody [EP5331] - BSA and Azide free (AB248130), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
Rat
Tested

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3'', and exhibits a preference towards 'Lys-48'-linked ubiquitin chains. Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome and is associated with neurogenerative disorders.

Alternative names

Ubiquitin carboxyl-terminal hydrolase isozyme L3, UCH-L3, Ubiquitin thioesterase L3, UCHL3

Recommended products

Rabbit Recombinant Monoclonal UCHL3 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EP5331
Purification technique
Affinity purification Protein A
Dissociation constant
5.27 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab248130 is the carrier-free version of Anti-UCHL3 antibody [EP5331] ab126621.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

UCHL3 also known as ubiquitin carboxyl-terminal hydrolase L3 is a deubiquitinating enzyme with a molecular mass of approximately 26 kDa. This protein plays a mechanical role in removing ubiquitin from polyubiquitinated proteins therefore controlling their degradation and recycling in the proteasome pathway. UCHL3 expresses widely across tissues with significant presence observed in the brain kidney and testis. It maintains protein homeostasis by ensuring that ubiquitin is available for various cellular processes making it an essential regulator of protein turnover.

Biological function summary

Ubiquitin carboxyl-terminal hydrolase L3 functions to regulate protein stability and signaling pathways by reversing protein ubiquitination. UCHL3 acts independently but can sometimes associate with complexes involved in cellular stress responses. Deubiquitinase activity makes it a critical regulator that modulates proteins involved in DNA repair transcriptional regulation and cell cycle progression. This regulation has vast implications for cellular growth differentiation and survival.

Pathways

UCHL3 plays significant roles in the ubiquitin-proteasome pathway and the DNA damage response pathway. By participating in these pathways UCHL3 influences cellular pathways that control protein quality control and genomic stability. It interacts with proteins such as p53 and BRCA1 influencing their stability and function. UCHL3's activity affects cellular responses to stress and repair processes thereby maintaining cellular integrity and promoting cancer prevention mechanisms.

Associated diseases and disorders

UCHL3 has connections to neurodegenerative disorders and cancer. The dysregulation of UCHL3 contributes to neurodegenerative diseases like Alzheimer's where protein aggregation occurs due to impaired ubiquitin processing. Its role in cancer includes influencing tumor suppressors and oncogenes through pathways involving proteins like p53 affecting cell cycle regulation and apoptosis. These associations make UCHL3 a relevant target for therapy development in these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - Anti-UCHL3 antibody [EP5331] - BSA and Azide free (ab248130), expandable thumbnail

    Western blot - Anti-UCHL3 antibody [EP5331] - BSA and Azide free (ab248130)

    Blocking/Diluting buffer: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-UCHL3 antibody [EP5331] ab126621).

    All lanes: Western blot - Anti-UCHL3 antibody [EP5331] (Anti-UCHL3 antibody [EP5331] ab126621) at 1/10000 dilution

    Lane 1: K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 15 µg

    Lane 2: Mouse brain lysate at 15 µg

    Lane 3: Rat brain lysate at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 26 kDa

    Observed band size: 26 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com