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Rabbit Monoclonal UCHL3 antibody. Suitable for WB, IP and reacts with Recombinant fragment - Human, Human, Mouse samples.

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Images

Western blot - Anti-UCHL3 antibody [EPR29841-548] (AB323384), expandable thumbnail
  • Western blot - Anti-UCHL3 antibody [EPR29841-548] (AB323384), expandable thumbnail
  • Immunoprecipitation - Anti-UCHL3 antibody [EPR29841-548] (AB323384), expandable thumbnail
  • Immunoprecipitation - Anti-UCHL3 antibody [EPR29841-548] (AB323384), expandable thumbnail
  • Western blot - Anti-UCHL3 antibody [EPR29841-548] (AB323384), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPIHC-P
Human
Tested
Tested
Not recommended
Mouse
Tested
Tested
Not recommended
Recombinant fragment - Human
Tested
Not recommended
Not recommended

Tested
Tested

Species
Recombinant fragment - Human
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Species
Mouse
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Recombinant fragment - Human
Dilution info
-
Notes

-

Target data

Function

Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3'', and exhibits a preference towards 'Lys-48'-linked ubiquitin chains. Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome and is associated with neurogenerative disorders.

Additional Targets

Uchl3

Alternative names

Ubiquitin carboxyl-terminal hydrolase isozyme L3, UCH-L3, Ubiquitin thioesterase L3, UCHL3

Rabbit Monoclonal UCHL3 antibody. Suitable for WB, IP and reacts with Recombinant fragment - Human, Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR29841-548
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-UCHL3 antibody [EPR29841-548] (ab323384), expandable thumbnail

    Western blot - Anti-UCHL3 antibody [EPR29841-548] (ab323384)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: TT.

    This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

    The identity of the bands higher 30 kDa are unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-UCHL3 antibody [EPR29841-548] (ab323384) at 1/1000 dilution

    Lane 1: F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate at 50 µg

    Lane 2: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 50 µg

    Lane 3: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 50 µg

    Lane 4: Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 50 µg

    Lane 5: TT (human thyroid carcinoma epithelial cell) whole cell lysate at 50 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Observed band size: 26 kDa, 36 kDa

    Exposure time: 92s

  • Western blot - Anti-UCHL3 antibody [EPR29841-548] (ab323384), expandable thumbnail

    Western blot - Anti-UCHL3 antibody [EPR29841-548] (ab323384)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-UCHL3 antibody [EPR29841-548] (ab323384) at 1/1000 dilution

    Lane 1: Human lung cancer tissue lysate at 20 µg

    Lane 2: Human pancreas cancer tissue lysate at 20 µg

    Lane 3: Human pancreas tissue lysate at 20 µg

    Lane 4: Mouse testis tissue lysate at 20 µg

    Lane 5: Mouse spleen tissue lysate at 20 µg

    Lane 6: Mouse pancreas tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 26 kDa, 36 kDa

    Exposure time: 180s

  • Immunoprecipitation - Anti-UCHL3 antibody [EPR29841-548] (ab323384), expandable thumbnail

    Immunoprecipitation - Anti-UCHL3 antibody [EPR29841-548] (ab323384)

    UCHL3 was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate with ab323384 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab323384 at 1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate

    Lane 2: ab323384 IP in Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab323384 in Neuro-2a whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST

    All lanes: Immunoprecipitation - Anti-UCHL3 antibody [EPR29841-548] (ab323384) at 1/30 dilution

    All lanes: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 10s

  • Immunoprecipitation - Anti-UCHL3 antibody [EPR29841-548] (ab323384), expandable thumbnail

    Immunoprecipitation - Anti-UCHL3 antibody [EPR29841-548] (ab323384)

    UCHL3 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab323384 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab323384 at 1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

    Lane 2: ab323384 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab323384 in Hela whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST

    All lanes: Immunoprecipitation - Anti-UCHL3 antibody [EPR29841-548] (ab323384) at 1/30 dilution

    All lanes: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 24s

  • Western blot - Anti-UCHL3 antibody [EPR29841-548] (ab323384), expandable thumbnail

    Western blot - Anti-UCHL3 antibody [EPR29841-548] (ab323384)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody does not cross-react with human UCHL1.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-UCHL3 antibody [EPR29841-548] (ab323384) at 1/1000 dilution

    Lane 1: His-tagged human UCHL3 recombinant protein at 10 ng

    Lane 2: His-tagged human UCHL1 recombinant protein at 10 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 23-28 kDa, 26 kDa

    Exposure time: 37s

  • Western blot - Anti-UCHL3 antibody [EPR29841-548] (ab323384), expandable thumbnail

    Western blot - Anti-UCHL3 antibody [EPR29841-548] (ab323384)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The identity of the bands higher 30 kDa and lower than 15 kDa are unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-UCHL3 antibody [EPR29841-548] (ab323384) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 50 µg

    Lane 2: HeLa transfected with siRNA specifically targeting UCHL3 whole cell lysate at 50 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 26 kDa, 36 kDa

    Exposure time: 37s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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