JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB231352

Anti-UCHL4 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal UCHL4 antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Mouse Uchl4 aa 1 to C-terminus.

View Alternative Names

Ubiquitin carboxyl-terminal hydrolase isozyme L4, UCH-L4, Ubiquitin thioesterase L4, Uchl4

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCHL4 antibody (AB231352)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCHL4 antibody (AB231352)

Formalin-fixed, paraffin-embedded mouse kidney tissue stained for UCHL4 using ab231352 at 20 μg/mL in immunohistochemical analysis.

Western blot - Anti-UCHL4 antibody (AB231352)
  • WB

Supplier Data

Western blot - Anti-UCHL4 antibody (AB231352)

All lanes:

Western blot - Anti-UCHL4 antibody (ab231352) at 3 µg/mL

All lanes:

Mouse lung lysate

Secondary

All lanes:

HRP-Linked Guinea pig anti-Rabbit at 1/1000 dilution

Predicted band size: 26 kDa

false

Western blot - Anti-UCHL4 antibody (AB231352)
  • WB

Supplier Data

Western blot - Anti-UCHL4 antibody (AB231352)

All lanes:

Western blot - Anti-UCHL4 antibody (ab231352) at 2 µg/mL

All lanes:

Recombinant mouse UCHL4 protein

Predicted band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Mouse Uchl4 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P58321

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UCHL4 also known as Ubiquitin C-terminal hydrolase L4 is a deubiquitinating enzyme with a mass of approximately 63 kDa. This protein is part of the ubiquitin-proteasome system which is vital in regulating protein degradation and turnover inside cells. UCHL4 is widely expressed in various tissues including the brain where it is involved in the processing of ubiquitinated proteins. As a hydrolase UCHL4 specifically removes ubiquitin from ubiquitinated protein substrates impacting protein stability and activity.
Biological function summary

Deubiquitinating enzymes like UCHL4 play a role in maintaining cellular homeostasis by regulating the levels of proteins targeted for degradation. UCHL4 associates with other proteins to form complexes involved in the recycling of ubiquitin a process essential for protein quality control. This function makes UCHL4 an important regulator in various cellular processes such as cell cycle progression and DNA repair mechanisms. By removing ubiquitin UCHL4 helps in rescuing proteins from degradation and can influence cellular signaling pathways.

Pathways

UCHL4 is involved in the ubiquitin-proteasome pathway (UPP) as well as the endosomal sorting complex required for transport (ESCRT). In the UPP UCHL4 works alongside proteins like the proteasome subunit beta type-7 (PSMB7) to mediate protein recycling and degradation. This pathway is essential for maintaining protein homeostasis within cells. In the ESCRT pathway UCHL4 assists in the sorting and trafficking processes critical for membrane dynamics and signaling.

Abnormal UCHL4 function is linked to neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease. UCHL4's regulatory role in protein degradation impacts the accumulation of protein aggregates in these disorders. Additionally its interaction with proteins like alpha-synuclein in Parkinson's disease highlights its importance in maintaining neural health. Understanding UCHL4's functional dynamics provides insights into the pathological mechanisms underlying these conditions and may suggest potential therapeutic targets.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Ubiquitin-protein hydrolase is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin.
See full target information Uchl4
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 22:1180 PubMed34475970

2021

Understanding the molecular role of syndecan-1 in the regulation of caspase-6 during the progression of cardiac arrhythmia.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanchu Wu,Lin Chen,Caihong Wang
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Secondary Antibodies

AB97180

Goat Anti-Rat IgM mu chain (HRP)

  • 5
  • 1
Proteins & Peptides

AB186081

Recombinant Human PPP3CA + PPP3R1 protein

SDS-PAGE - Recombinant Human PPP3CA + PPP3R1 protein (AB186081)

  • 0
  • 0
Proteins & Peptides

AB140719

Recombinant Human MRM1 protein

SDS-PAGE - Recombinant Human MRM1 protein (AB140719)

  • 0
  • 0
Proteins & Peptides

AB155609

Recombinant human LIFR protein (Active)

Bioactive

ELISA - Recombinant human LIFR protein (Active) (AB155609)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com