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Rabbit Recombinant Monoclonal UCHL5IP antibody. Suitable for IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse samples. Cited in 1 publication.

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Images

Western blot - Anti-UCHL5IP antibody [EPR15341-56] (AB192616), expandable thumbnail
  • Immunoprecipitation - Anti-UCHL5IP antibody [EPR15341-56] (AB192616), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCHL5IP antibody [EPR15341-56] (AB192616), expandable thumbnail
  • Western blot - Anti-UCHL5IP antibody [EPR15341-56] (AB192616), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCHL5IP antibody [EPR15341-56] (AB192616), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Tested

Tested
Tested

Species
Human
Dilution info
1/150
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/480
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/250 - 1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/250 - 1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex.

Alternative names

UCHL5IP, UIP1, HAUS7, HAUS augmin-like complex subunit 7, 26S proteasome-associated UCH37-interacting protein 1, UCHL5-interacting protein, X-linked protein STS1769

Recommended products

Rabbit Recombinant Monoclonal UCHL5IP antibody. Suitable for IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR15341-56
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

UCHL5IP also known as Ubiquitin Carboxyl-terminal Hydrolase L5 Interacting Protein functions as an important protein supporting the role of UCHL5 in protein degradation. UCHL5IP is involved in deubiquitination processes and has closely-related functions with enzymes in removing ubiquitin from proteins. Its molecular mass is approximately 26 kDa. UCHL5IP is broadly expressed in various tissues suggesting its involvement in several cellular processes.

Biological function summary

UCHL5IP regulates protein turnover by assisting the function of UCHL5. It forms part of a complex with the proteasome which is important for maintaining protein homeostasis. This interaction helps the proteasome properly degrade misfolded or excessive proteins enhancing cellular regulation. The involvement of UCHL5IP in maintaining protein quality control underlines its significance in cellular integrity and function.

Pathways

Scientists recognize UCHL5IP's participation in the ubiquitin-proteasome pathway a major cellular mechanism controlling protein degradation. It interacts with UCHL5 a component of the protease interacting with the proteasome. The pathway is responsible for regulating protein levels and maintaining cellular balance. Additionally UCHL5IP might have connections to signaling pathways through roles in modulating protein levels linking it to broader networks of cellular communication and response.

Associated diseases and disorders

Researchers have linked UCHL5IP to neurodegenerative diseases due to its involvement in proteasome function. Malfunction in protein degradation pathways contributes to disorders such as Parkinson’s disease. UCHL5IP through its interaction with UCHL5 can influence the build-up of misfolded proteins which are necessary for disease development. Continued study on UCHL5IP might provide further insights into its role in these conditions and potential therapeutic targets.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-UCHL5IP antibody [EPR15341-56] (ab192616), expandable thumbnail

    Western blot - Anti-UCHL5IP antibody [EPR15341-56] (ab192616)

    All lanes: Western blot - Anti-UCHL5IP antibody [EPR15341-56] (ab192616) at 1/1000 dilution

    Lane 1: HeLa cell lysate at 20 µg

    Lane 2: Jurkat cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 40 kDa

  • Immunoprecipitation - Anti-UCHL5IP antibody [EPR15341-56] (ab192616), expandable thumbnail

    Immunoprecipitation - Anti-UCHL5IP antibody [EPR15341-56] (ab192616)

    Western blot analysis of immunoprecipitation pellet from Jurkat cell lysate immunoprecipitated using ab192616 at 1/150 dilution (lane 1) or PBS control (lane 2).
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

    All lanes: Immunoprecipitation - Anti-UCHL5IP antibody [EPR15341-56] (ab192616)

    Predicted band size: 40 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCHL5IP antibody [EPR15341-56] (ab192616), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCHL5IP antibody [EPR15341-56] (ab192616)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling UCHL5IP with ab192616 at 1/500 dilution (5μg/ml) dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-UCHL5IP antibody [EPR15341-56] (ab192616), expandable thumbnail

    Western blot - Anti-UCHL5IP antibody [EPR15341-56] (ab192616)

    All lanes: Western blot - Anti-UCHL5IP antibody [EPR15341-56] (ab192616) at 1/1000 dilution

    All lanes: RAW 264.7 cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 25 kDa, 31 kDa, 40 kDa, 48 kDa, 50 kDa, 54 kDa, 84 kDa, 90 kDa

    Observed band size: 50 kDa, 54 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCHL5IP antibody [EPR15341-56] (ab192616), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCHL5IP antibody [EPR15341-56] (ab192616)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling UCHL5IP with ab192616 at 1/500 dilution (5μg/ml) followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCHL5IP antibody [EPR15341-56] (ab192616), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCHL5IP antibody [EPR15341-56] (ab192616)

    Immunohistochemical analysis of paraffin-embedded Human endometrium adenocarcinoma tissue labeling UCHL5IP with ab192616 at 1/500 dilution (5μg/ml) followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-UCHL5IP antibody [EPR15341-56] (ab192616), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-UCHL5IP antibody [EPR15341-56] (ab192616)

    Intracellular flow cytometric analysis of Jurkat cells (paraformaldehyde-fixed, 2%)labeling UCHL5IP with ab192616 at 1/480 dilution (red) or a rabbit IgG (negative) (black), unlabeled cells (blue) followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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