AB241281

Anti-UCK2 antibody

Name: Anti-UCK2 antibody (ab241281) | Abcam
Brand: Abcam Limited
SKU: AB241281

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(4 Publications)

Rabbit Polyclonal UCK2 antibody. Suitable for IP and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human UCK2 aa 1-50.

View Alternative Names

UMPK, UCK2, Uridine-cytidine kinase 2, UCK 2, Cytidine monophosphokinase 2, Testis-specific protein TSA903, Uridine monophosphokinase 2

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Supplier Data

Immunoprecipitation - Anti-UCK2 antibody (AB241281)

UCK2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded, prepared using NETN lysis buffer) using ab241281 at 6 μg per reaction. Western blot was performed on the immunoprecipitate using ab241281 at 1 μg/ml.

Lane 1 : ab241281 IP in HeLa whole cell lysate.
Lane 2 : Control IgG.

Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-UCK2 antibody (ab241281)

Predicted band size: 29 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human UCK2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg", "IP-species-notes": "<p></p>" } } }

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Product details

ab241281 has not performed satisfactorily when used for WB of UCK2 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of UCK2.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab241281 was affinity purified using an epitope specific to UCK2 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Uridine-cytidine kinase 2 (UCK2) also known as CaCK2 is an enzyme responsible for catalyzing the phosphorylation of uridine and cytidine to their respective monophosphate forms. It has a molecular mass of about 30 kDa. Expressed in various tissues UCK2 is particularly active in tumor tissues where it plays a role in nucleoside metabolism. Its activity is important for the salvage pathways recycling pyrimidine bases and maintaining nucleotide pool balance in cells.

Biological function summary

UCK2 serves a critical role in nucleotide metabolism by converting nucleosides into nucleotides which are essential for nucleic acid synthesis and cellular proliferation. UCK2 is not part of a larger protein complex but interacts closely with other enzymes in the salvage pathway. The enzymatic activity of UCK2 allows cells to maintain efficient pyrimidine recycling which is important for cellular energy balance and DNA replication.

Pathways

Uridine-cytidine kinase 2 participates mainly in the pyrimidine salvage pathway. It is linked to the nucleotide biosynthesis pathway ensuring a supply of nucleotides independent of de novo synthesis. UCK2 interacts with related enzymes such as cytidine deaminase and uridine phosphorylase which also play key roles in these pathways. The proper function of this pathway supports DNA replication and repair processes which are essential to maintain cell division and function.

UCK2 is often associated with various cancers including pancreatic and colorectal cancer. Raised expression of UCK2 is observed in tumors linking it to cell proliferation. The enzyme's role in cancer development involves its interaction and overlap with proteins such as thymidylate synthase which are important for DNA synthesis and repair. Abnormal UCK2 activity or expression levels can contribute to tumor growth and resistance to certain chemotherapy drugs that target nucleotide metabolism.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Phosphorylates uridine and cytidine to uridine monophosphate and cytidine monophosphate (PubMed : 11306702, PubMed : 11494055). Does not phosphorylate deoxyribonucleosides or purine ribonucleosides (PubMed : 11306702). Can use ATP or GTP as a phosphate donor (PubMed : 11306702). Can also phosphorylate cytidine and uridine nucleoside analogs such as 6-azauridine, 5-fluorouridine, 4-thiouridine, 5-bromouridine, N(4)-acetylcytidine, N(4)-benzoylcytidine, 5-fluorocytidine, 2-thiocytidine, 5-methylcytidine, and N(4)-anisoylcytidine (PubMed : 11306702).

See full target information UCK2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Translational oncology 58:102423 PubMed40414119

2025

GLI2-HRD1 axis facilitates 5-FU resistance in gastric cancer cells by regulating ubiquitination degradation of UCK2.

Applications

Unspecified application

Species

Unspecified reactive species

Chaorong Xue,Xuanzi Zhang,Wanling Yu,Hanbin Lin,Junrong Zhang,Jiawen Liu,Zongqi Weng,Manduo Ouyang,Xinjian Lin

Cell death discovery 10:375 PubMed39179560

2024

UCK2 promotes intrahepatic cholangiocarcinoma progression and desensitizes cisplatin treatment by PI3K/AKT/mTOR/autophagic axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiwen Wu,Da Chen,Muqi Li,Gehao Liang,Huizhen Ye

Cellular & molecular biology letters 27:105 PubMed36447138

2022

Targeting uridine-cytidine kinase 2 induced cell cycle arrest through dual mechanism and could improve the immune response of hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dehai Wu,Congyi Zhang,Guanqun Liao,Kaiming Leng,Bowen Dong,Yang Yu,Huilin Tai,Lining Huang,Feng Luo,Bin Zhang,Tiexiang Zhan,Qiuhui Hu,Sheng Tai

Molecular metabolism 53:101249 PubMed33989779

2021

Histone H3K9 butyrylation is regulated by dietary fat and stress via an Acyl-CoA dehydrogenase short chain-dependent mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi Yang,Minzhen He,Julianne Austin,Jessica Pfleger,Maha Abdellatif

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-UCK2 antibody

Immunoprecipitation - Anti-UCK2 antibody (AB241281)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise