Rabbit Recombinant Monoclonal UCK2 antibody. Suitable for WB and reacts with Human, Transfected cell lysate - Human, Mouse samples.
Images
![Western blot - Anti-UCK2 antibody [EPR27020-82] (AB308252), expandable thumbnail](https://content.abcam.com/products/images/uck2-antibody-epr27020-82-ab308252--western-blot-img408863.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-UCK2 antibody [EPR27020-82] (AB308252)")
![Western blot - Anti-UCK2 antibody [EPR27020-82] (AB308252), expandable thumbnail](https://content.abcam.com/products/images/uck2-antibody-epr27020-82-ab308252--western-blot-img408862.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-UCK2 antibody [EPR27020-82] (AB308252)")
![Western blot - Anti-UCK2 antibody [EPR27020-82] (AB308252), expandable thumbnail](https://content.abcam.com/products/images/uck2-antibody-epr27020-82-ab308252--western-blot-img408861.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-UCK2 antibody [EPR27020-82] (AB308252)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt (Intra) | IP | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Transfected cell lysate - Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Transfected cell lysate - Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Transfected cell lysate - Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Transfected cell lysate - Human | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Transfected cell lysate - Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Transfected cell lysate - Human | Dilution info - | Notes - |
Target data
Function
Phosphorylates uridine and cytidine to uridine monophosphate and cytidine monophosphate (PubMed:11306702, PubMed:11494055). Does not phosphorylate deoxyribonucleosides or purine ribonucleosides (PubMed:11306702). Can use ATP or GTP as a phosphate donor (PubMed:11306702). Can also phosphorylate cytidine and uridine nucleoside analogs such as 6-azauridine, 5-fluorouridine, 4-thiouridine, 5-bromouridine, N(4)-acetylcytidine, N(4)-benzoylcytidine, 5-fluorocytidine, 2-thiocytidine, 5-methylcytidine, and N(4)-anisoylcytidine (PubMed:11306702).
Alternative names
UMPK, UCK2, Uridine-cytidine kinase 2, UCK 2, Cytidine monophosphokinase 2, Testis-specific protein TSA903, Uridine monophosphokinase 2
Recommended products
Rabbit Recombinant Monoclonal UCK2 antibody. Suitable for WB and reacts with Human, Transfected cell lysate - Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR27020-82
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Uridine-cytidine kinase 2 (UCK2) also known as CaCK2 is an enzyme responsible for catalyzing the phosphorylation of uridine and cytidine to their respective monophosphate forms. It has a molecular mass of about 30 kDa. Expressed in various tissues UCK2 is particularly active in tumor tissues where it plays a role in nucleoside metabolism. Its activity is important for the salvage pathways recycling pyrimidine bases and maintaining nucleotide pool balance in cells.
- Biological function summary
UCK2 serves a critical role in nucleotide metabolism by converting nucleosides into nucleotides which are essential for nucleic acid synthesis and cellular proliferation. UCK2 is not part of a larger protein complex but interacts closely with other enzymes in the salvage pathway. The enzymatic activity of UCK2 allows cells to maintain efficient pyrimidine recycling which is important for cellular energy balance and DNA replication.
- Pathways
Uridine-cytidine kinase 2 participates mainly in the pyrimidine salvage pathway. It is linked to the nucleotide biosynthesis pathway ensuring a supply of nucleotides independent of de novo synthesis. UCK2 interacts with related enzymes such as cytidine deaminase and uridine phosphorylase which also play key roles in these pathways. The proper function of this pathway supports DNA replication and repair processes which are essential to maintain cell division and function.
- Associated diseases and disorders
UCK2 is often associated with various cancers including pancreatic and colorectal cancer. Raised expression of UCK2 is observed in tumors linking it to cell proliferation. The enzyme's role in cancer development involves its interaction and overlap with proteins such as thymidylate synthase which are important for DNA synthesis and repair. Abnormal UCK2 activity or expression levels can contribute to tumor growth and resistance to certain chemotherapy drugs that target nucleotide metabolism.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-UCK2 antibody [EPR27020-82] (ab308252), expandable thumbnail](https://content.abcam.com/products/images/uck2-antibody-epr27020-82-ab308252--western-blot-img408863.jpg "Western blot - Anti-UCK2 antibody [EPR27020-82] (ab308252)")
Western blot - Anti-UCK2 antibody [EPR27020-82] (ab308252)
UCK2 Western blot staining using rabbit Anti-UCK2 antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This antibody does not cross-react with human UCK1.
In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
In Western blot, anti- Vinculin antibody (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) loading control staining at 1/10000 dilution.
Exposure time: 6 seconds.All lanes: Western blot - Anti-UCK2 antibody [EPR27020-82] (ab308252) at 1/1000 dilution
Lane 1: 293T cells transfected with an empty vector containing a His tag whole cell lysate at 20 µg
Lane 2: 293T cells transfected with a Human UCK2 expression vector containing a His-tag whole cell lysate at 20 µg
Lane 3: 293T cells transfected with a Human UCK1 expression vector containing a His-tag whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 30 kDa
![Western blot - Anti-UCK2 antibody [EPR27020-82] (ab308252), expandable thumbnail](https://content.abcam.com/products/images/uck2-antibody-epr27020-82-ab308252--western-blot-img408862.jpg "Western blot - Anti-UCK2 antibody [EPR27020-82] (ab308252)")
Western blot - Anti-UCK2 antibody [EPR27020-82] (ab308252)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Positive control: YAC-1(PMID: 35583288).
Lane 2 was freshly made and used for Western blotting immediately to minimize protein degradation.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 180 seconds.All lanes: Western blot - Anti-UCK2 antibody [EPR27020-82] (ab308252) at 1/1000 dilution
Lane 1: YAC-1 (mouse Moloney leukemia virus induced lymphoma lymphoblast) whole cell lysate at 20 µg
Lane 2: Mouse placenta tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
![Western blot - Anti-UCK2 antibody [EPR27020-82] (ab308252), expandable thumbnail](https://content.abcam.com/products/images/uck2-antibody-epr27020-82-ab308252--western-blot-img408861.jpg "Western blot - Anti-UCK2 antibody [EPR27020-82] (ab308252)")
Western blot - Anti-UCK2 antibody [EPR27020-82] (ab308252)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: Huh7 and U-2 OS(PMID: 35583288).
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 92 seconds.All lanes: Western blot - Anti-UCK2 antibody [EPR27020-82] (ab308252) at 1/1000 dilution
Lane 1: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg
Lane 2: Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 20 µg
Lane 3: Huh7 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 30 kDa
Downloads
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