Anti-UCK2 antibody [EPR27020-82] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-UCK2 antibody [EPR27020-82] - BSA and Azide free (AB308253)

This data was produced using ab308252, the same clone but in a different formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. Low expression : Huh7 and U-2 OS(PMID : 35583288). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 92 seconds.

All lanes:

Western blot - Anti-UCK2 antibody [EPR27020-82] (<a href='/en-us/products/primary-antibodies/uck2-antibody-epr27020-82-ab308252'>ab308252</a>) at 1/1000 dilution

Lane 1:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Lane 2:

Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

Lane 3:

Huh7 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 30 kDa

false

• WB

Supplier Data

Western blot - Anti-UCK2 antibody [EPR27020-82] - BSA and Azide free (AB308253)

This data was produced using ab308252, the same clone but in a different formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. This antibody does not cross-react with human UCK1. In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution. In Western blot, anti- Vinculin antibody (ab129002) loading control staining at 1/10000 dilution. Exposure time : 6 seconds.

All lanes:

Western blot - Anti-UCK2 antibody [EPR27020-82] (<a href='/en-us/products/primary-antibodies/uck2-antibody-epr27020-82-ab308252'>ab308252</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a His tag whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a Human UCK2 expression vector containing a His-tag whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a Human UCK1 expression vector containing a His-tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 30 kDa

false

• WB

Supplier Data

Western blot - Anti-UCK2 antibody [EPR27020-82] - BSA and Azide free (AB308253)

This data was produced using ab308252, the same clone but in a different formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. Positive control : YAC-1(PMID : 35583288). Lane 2 was freshly made and used for Western blotting immediately to minimize protein degradation. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 180 seconds.

All lanes:

Western blot - Anti-UCK2 antibody [EPR27020-82] (<a href='/en-us/products/primary-antibodies/uck2-antibody-epr27020-82-ab308252'>ab308252</a>) at 1/1000 dilution

Lane 1:

YAC-1 (mouse Moloney leukemia virus induced lymphoma lymphoblast) whole cell lysate at 20 µg

Lane 2:

Mouse placenta tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false