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AB126754

Anti-UCKL1 antibody [EPR7384]

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(1 Publication)

Rabbit Recombinant Monoclonal UCKL1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

URKL1, F538, UCKL1, Uridine-cytidine kinase-like 1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCKL1 antibody [EPR7384] (AB126754)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCKL1 antibody [EPR7384] (AB126754)

ab126754 at 1/100 dilution staining UCKL1 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-UCKL1 antibody [EPR7384] (AB126754)
  • WB

Unknown

Western blot - Anti-UCKL1 antibody [EPR7384] (AB126754)

All lanes:

Western blot - Anti-UCKL1 antibody [EPR7384] (ab126754) at 1/1000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit at 1/2000 dilution

Predicted band size: 61 kDa

false

OI-RD Scanning - Anti-UCKL1 antibody [EPR7384] (AB126754)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-UCKL1 antibody [EPR7384] (AB126754)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-UCKL1 antibody [EPR7384] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7384

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Western blot - Anti-UCK2 antibody [EPR27020-82] (AB308252)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARA antibody [EPR5020] - BSA and Azide free (AB248032)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Uridine-cytidine kinase-like 1 (UCKL1) also known as FLJ10540 is an enzyme with a molecular mass of approximately 38 kDa. It catalyzes the phosphorylation of uridine and cytidine to their corresponding monophosphate forms. UCKL1 localizes in the cytoplasm and nucleus of cells and expresses highly in testis tissues but also appears in liver pancreas and certain tumor cells. Its ability to phosphorylate nucleosides makes it an important player in nucleotide metabolism.
Biological function summary

UCKL1 plays a significant role in cellular nucleotide synthesis. It supports the salvage pathways which provide an efficient route for nucleotide production. This protein is not part of a larger complex but interacts with other nucleotide synthesis enzymes to maintain adequate levels of nucleotides in cells. Through these interactions it helps regulate cellular proliferation by ensuring adequate nucleotide supply for DNA and RNA synthesis.

Pathways

UCKL1 is involved in nucleotide metabolism with particular importance in the pyrimidine salvage pathway. It works closely with other kinases and enzymes like nucleoside diphosphate kinases which further phosphorylate the monophosphates. This salvage pathway complements the de novo synthesis of nucleotides ensuring rapid cell proliferation required during cell growth and repair.

UCKL1 has associations with cancer development and progression particularly in hepatocellular carcinoma. Its overexpression links to increased cellular proliferation and tumor progression. UCKL1 may intersect with proteins like p53 influencing cancer cell survival pathways. Additionally high levels of UCKL1 correlate with autoimmune disorders although the precise mechanism is not fully understood. The binding interactions of UCKL1 with pathological proteins require further research to clarify these disease connections.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May contribute to UTP accumulation needed for blast transformation and proliferation.
See full target information UCKL1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Immunity, inflammation and disease 11:e852 PubMed37904692

2023

The potential roles of stress-induced phosphoprotein 1 and connexin 43 in rats with reperfusion arrhythmia.

Applications

Unspecified application

Species

Unspecified reactive species

Li An,Hong Gao,Yi Zhong,Yanqiu Liu,Ying Cao,Jing Yi,Xiang Huang,Chunlei Wen,Rui Tong,Zhijun Pan,Xu Yan,Meiyan Liu,Shengzhao Wang,Hao Wu,Tingju Hu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com