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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit Recombinant Monoclonal UCKL1 antibody. Suitable for IHC-P, WB and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Liquid
Monoclonal
IHC-P | ICC/IF | IP | Flow Cyt | WB | |
---|---|---|---|---|---|
Human | Tested | Not recommended | Not recommended | Not recommended | Tested |
Mouse | Predicted | Not recommended | Not recommended | Not recommended | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
May contribute to UTP accumulation needed for blast transformation and proliferation.
Uridine-cytidine kinase-like 1, URKL1, UCKL1, F538
Rabbit Recombinant Monoclonal UCKL1 antibody. Suitable for IHC-P, WB and reacts with Human samples.
Uridine-cytidine kinase-like 1, URKL1, UCKL1, F538
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Liquid
Monoclonal
EPR7384
Affinity purification Protein A
5.3 x 10-11 M
Blue Ice
-20°C
Stable for 12 months at -20°C
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
UCKL1 plays a significant role in cellular nucleotide synthesis. It supports the salvage pathways which provide an efficient route for nucleotide production. This protein is not part of a larger complex but interacts with other nucleotide synthesis enzymes to maintain adequate levels of nucleotides in cells. Through these interactions it helps regulate cellular proliferation by ensuring adequate nucleotide supply for DNA and RNA synthesis.
Uridine-cytidine kinase-like 1 (UCKL1) also known as FLJ10540 is an enzyme with a molecular mass of approximately 38 kDa. It catalyzes the phosphorylation of uridine and cytidine to their corresponding monophosphate forms. UCKL1 localizes in the cytoplasm and nucleus of cells and expresses highly in testis tissues but also appears in liver pancreas and certain tumor cells. Its ability to phosphorylate nucleosides makes it an important player in nucleotide metabolism.
UCKL1 is involved in nucleotide metabolism with particular importance in the pyrimidine salvage pathway. It works closely with other kinases and enzymes like nucleoside diphosphate kinases which further phosphorylate the monophosphates. This salvage pathway complements the de novo synthesis of nucleotides ensuring rapid cell proliferation required during cell growth and repair.
UCKL1 has associations with cancer development and progression particularly in hepatocellular carcinoma. Its overexpression links to increased cellular proliferation and tumor progression. UCKL1 may intersect with proteins like p53 influencing cancer cell survival pathways. Additionally high levels of UCKL1 correlate with autoimmune disorders although the precise mechanism is not fully understood. The binding interactions of UCKL1 with pathological proteins require further research to clarify these disease connections.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
All lanes: Western blot - Anti-UCKL1 antibody [EPR7384] (AB126754) at 1/1000 dilution
Lane 1: 293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg
Lane 2: HeLa cell lysate at 10 µg
All lanes: HRP-conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size: 61 kDa
ab126754, at 1/100 dilution, staining UCKL1 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com