AB10983

Anti-UCP1 antibody

Name: UCP1 antibody, rabbit polyclonal (ab10983) | Abcam
Brand: Abcam Limited
SKU: AB10983
Price: 520 USD
Availability: InStock
Rating: 4 (23 reviews)

(23 Reviews)

(975 Publications)

Anti-UCP1 antibody (ab10983) is a rabbit polyclonal antibody detecting UCP1 in Western Blot, IHC-P. Suitable for Mouse, Rat.

- Over 810 publications
- Trusted since 2004

View Alternative Names

SLC25A7, UCP, UCP1, Mitochondrial brown fat uncoupling protein 1, UCP 1, Solute carrier family 25 member 7, Thermogenin

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody (AB10983)

IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody (AB10983)

ab10983, staining UCP1, in murine inguinal white adipose tissue, by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Tissue was incubated with primary antibody at 1/1000 dilution and staining was detected using an HRP/DAB detection kit. Sections were counterstained using hematoxylin.

Image from Mori M et al., PLoS Biol. 2012;10(4):e1001314. Epub 2012 Apr 24. Fig 5.; doi:10.1371/journal.pbio.1001314; April 24, 2012, PLoS Biol 10(4): e1001314.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody (AB10983)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse brown adipose tissue staining UCP1 using ab10983 at 1/500 dilution. Detected using an Anti-Rabbit IgG-biotin conjugate and an Avidin-Peroxidase conjugate.

Supplier Data

Western blot - Anti-UCP1 antibody (AB10983)

All lanes:

Western blot - Anti-UCP1 antibody (ab10983) at 1 µg/mL

Lane 1:

Rat brown fat

Lane 2:

Rat brown fat with UCP1 immunizing peptide (human, 145-159)

Secondary

All lanes:

Goat Anti-Rabbit IgG-Alkaline Phosphatase and a colorimetric substrate

Predicted band size: 33 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human UCP1 aa 100-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P25874

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p>This concentration is determined by indirect immunoperoxidase staining of protease digested, formalin-fixed, paraffin-embedded sections of mouse brown adipose tissue.</p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p>Use at a minimum dilution of 1/1000 in extract of rat brown adipose tissue (BAT) mitochondria or an extract of E.coli expressing recombinant mouse UCP1. Additional weak bands may be detected in some preparations of BAT extracts. Staining of the UCP1 band is specifically inhibited with the immunizing peptide. This antibody may not be suitable for Western blot in Human samples.</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Macaque monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Product Specifications
Anti-UCP1 antibody (ab10983) is a rabbit polyclonal antibody and is validated for use in IHC-P, WB in mouse, rat samples.
Anti-UCP1 antibody (ab10983) specifically detects UCP1 (UniProt ID: P25874; Molecular weight: 33kDa) and is sold in 50 µL selling sizes.

Quality and Validation
Abcam's high quality validation processes ensure Anti-UCP1 antibody (ab10983) has high sensitivity and specificity.
Anti-UCP1 antibody (ab10983) has been cited over 814 times in peer reviewed journals and is trusted by the scientific community.
Anti-UCP1 antibody (ab10983) has 23 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab10983 is affinity purified using the immunogenic peptide immobilized on agarose.

Storage buffer

Preservative: 0.097% Sodium azide Constituents: PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Uncoupling Protein 1 (UCP1) also known as thermogenin is an important protein found in the mitochondria of brown adipose tissue (BAT). It has a molecular mass of approximately 33 kDa. UCP1 functions as a mitochondrial carrier specifically involved in the process of proton transport across the inner mitochondrial membrane. It leads to the dissipation of the proton gradient producing heat instead of ATP. This process is called non-shivering thermogenesis. UCP1 is mainly expressed in brown adipose tissue an area important for heat production in mammals.

Biological function summary

UCP1 plays an essential role in energy metabolism by activating thermogenesis in brown adipocytes. It does not form a larger multi-protein complex but acts as an independent protein facilitating a significant amount of heat production. By uncoupling oxidative phosphorylation UCP1 controls energy expenditure and is central to maintaining body temperature in cold environments. Its function regulates metabolic rate and prevents excess fat accumulation connecting with mechanisms involved in energy balance.

Pathways

UCP1 is an integral component of the metabolic pathway for thermogenesis. The PPAR signaling pathway regulates its expression emphasizing its role in energy homeostasis. Additionally UCP1 interacts with proteins like PGC-1α an important regulator of energy metabolism and mitochondrial biogenesis. This interaction highlights UCP1's involvement in mitochondrial activity and adaptation to changes in temperature or diet linking it to important metabolic functions.

UCP1 has been connected with obesity and metabolic syndrome. Its dysregulation can lead to impaired thermogenic responses and reduced metabolic rate contributing to metabolic disorders. UCP1 is related to leptin a protein that regulates appetite and energy balance which is impacted in cases of obesity. Understanding this relationship helps in exploring potential therapeutic targets for tackling obesity and related metabolic diseases by modulating UCP1 activity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mitochondrial protein responsible for thermogenic respiration, a specialized capacity of brown adipose tissue and beige fat that participates in non-shivering adaptive thermogenesis to temperature and diet variations and more generally to the regulation of energy balance (By similarity). Functions as a long-chain fatty acid/LCFA and proton symporter, simultaneously transporting one LCFA and one proton through the inner mitochondrial membrane (PubMed : 24196960, PubMed : 28781081). However, LCFAs remaining associated with the transporter via their hydrophobic tails, it results in an apparent transport of protons activated by LCFAs. Thereby, dissipates the mitochondrial proton gradient and converts the energy of substrate oxydation into heat instead of ATP. Regulates the production of reactive oxygen species/ROS by mitochondria (By similarity).

See full target information UCP1

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Publications (975)

Recent publications for all applications. Explore the full list and refine your search

GeroScience : PubMed41075110

2025

GHSR suppression in neurons protects against aging-associated metabolic and cognitive impairments.

Applications

Unspecified application

Species

Unspecified reactive species

Hongying Wang,Chia-Shan Wu,Danilo Landrock,Ariel Nevarez,Shanrun Liu,Anna Thalacker-Mercer,Yanfeng Zhang,Jamie I Baum,Sunja Kim,Bingzhong Xue,Yuxiang Sun

Nature communications 16:8406 PubMed40998774

2025

Cryo-EM structure of the full-length LGR4-RSPOs complex and a targeting nanobody for anti-obesity therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongyun Zhang,Lu Wang,Huarui Qiao,Haowen Jiang,Shaojue Guo,Yuying Li,Ningning Zhang,Tengjie Geng,Qianqian Cui,Zhongyun Lan,Jie Hong,Weiqiong Gu,Ruixin Liu,Guang Ning,Jia Li,Jiqiu Wang,Yong Geng

Nature communications 16:7033 PubMed40908286

2025

Identification of Neuritin 1 as a local metabolic regulator of brown adipose tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Manuela Sánchez-Feutrie,Montserrat Romero,Sónia R Veiga,Núria Borràs-Ferré,Nick Berrow,Martina Ràfols,Noemí Giménez,Andrea Rodgers-Furones,Alba Sabaté-Pérez,Ángela Rodríguez Pérez,Luis Rodrigo Cataldo,Hans Burghardt,David Sebastián,Natàlia Plana,Vanessa Hernández,Laura Isabel Alcaide,Óscar Reina,Maria J Monte,José Juan G Marin,Manuel Palacín,Remy Burcelin,Per Antonson,Jan-Ake Gustafsson,Antonio Zorzano

Scientific reports 15:31922 PubMed40883493

2025

Car4-positive adipocyte progenitor cells adapt to the aging environment and work as protection against ROS via glutathione metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Masato Horino,Kenji Ikeda,Rei Okazaki,Yujiro Nakano,Ryo Kaneda,Ryoko Ishii,Jun Aoki,Kazunari Hara,Akira Takeuchi,Yoshihiro Niitsu,Kuniyuki Taguchi,Kuniyuki Kano,Masanori Murakami,Kazutaka Tsujimoto,Chikara Komiya,Tetsuya Yamada

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 39:e70886 PubMed40787784

2025

The TBLR1/TBL1 Co-Factor Complex Acts as a Transcriptional Checkpoint in the Brown Adipose Tissue Response to Prolonged Cold Exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Sahika Cingir Köker,Foivos-Filippos Tsokanos,Rabih El-Merahbi,Ankush Kumar Jha,Karin Cicatelli,Peter Weber,Amit Mhamane,Doris Kaltenecker,Pauline Morigny,Anne Loft,Katarina Klepac,Adriano Maida,Claudia-Eveline Molocea,Daniela Hass,Elena Sophie Vogl,Ana Jimena Alfaro,Wenfei Sun,Horst Zitzelsberger,Kristian Unger,Julia Szendrödi,Yongguo Li,Mauricio Berriel Diaz,Christian Wolfrum,Alexander Bartelt,Stephan Herzig,Anastasia Georgiadi

Journal of physiology and biochemistry : PubMed40760244

2025

Sirtuin 1 overexpression in mice preserves insulin and thermogenic responses in subcutaneous inguinal white adipose tissue under proinflammatory conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Patricia Vázquez,Carmen Escalona-Garrido,Nuria Pescador,Ana B Hitos,Daniel González-Moreno,Ángela de Benito-Bueno,Elena Sierra-Filardi,Patricia Boya,Ana Montero-Pedrazuela,Ana Guadaño-Ferraz,Ángela M Valverde

Nature microbiology 10:1918-1939 PubMed40745048

2025

Polypeptides synthesized by common bacteria in the human gut improve rodent metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Fan,Liwei Lyu,Ruben Vazquez-Uribe,Wanliang Zhang,Mareike Bongers,Andreas Koulouktsis,Mengliu Yang,Vita Sereika-Bejder,Tulika Arora,Evelina Stankevic,Jeremy Armetta,Franziska Zosel,Charlotta D de la Cour,Lotte Simonsen,Alina Kulakova,Michael Wierer,Pernille Harris,Joachim Gæde,Peter Rossing,Filip K Knop,Tune H Pers,Tue Haldor Hansen,Trine Nielsen,Ling Li,Kristian Strømgaard,Gangyi Yang,Morten Otto Alexander Sommer,Oluf Pedersen

Food chemistry: X 29:102679 PubMed40612770

2025

Rebaudioside D and M, the next-generation sugar substitutes, do not exacerbate metabolic dysfunction in high-fat diet mice.

Applications

Unspecified application

Species

Unspecified reactive species

Sangyi Lim,Min-Jung Park,Ki-Nam Yoon,Junhyeong Lee,Merc Emil Matienzo,Seong-Jin Hong,Keon Kim,Yeong-Bin Baek,Chang-Min Lee,Young-Min Kim,Bu-Soo Park,Dong-Il Kim

Molecular metabolism 99:102197 PubMed40582590

2025

An epigenome atlas of mouse adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Laura C Hinte,Adhideb Ghosh,Daniel Castellano-Castillo,Christian Wolfrum,Ferdinand von Meyenn

Cytotechnology 77:125 PubMed40529474

2025

Falcarindiol promotes beige adipocyte-related gene expression and mitochondrial respiration in human preadipocyte-derived adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Shingo Takahashi,Haruka Okaze,Seiji Kawamoto

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-UCP1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody (AB10983)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody (AB10983)

Western blot - Anti-UCP1 antibody (AB10983)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reagents, Controls & Accessories

Secondary Antibodies

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (975)

Product promise