AB155117

Anti-UCP1 antibody

Name: Anti-UCP1 antibody (ab155117) | Abcam
Brand: Abcam Limited
SKU: AB155117
Price: 500 USD
Availability: InStock

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(49 Publications)

Anti-UCP1 antibody (ab155117) is a rabbit polyclonal antibody detecting UCP1 in Western Blot, IHC-P. Suitable for Human, Mouse.

- Over 40 publications

View Alternative Names

SLC25A7, UCP, UCP1, Mitochondrial brown fat uncoupling protein 1, UCP 1, Solute carrier family 25 member 7, Thermogenin

2 Images

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Western blot - Anti-UCP1 antibody (AB155117)

10% SDS PAGE

All lanes:

Western blot - Anti-UCP1 antibody (ab155117) at 1/1000 dilution

All lanes:

Mouse interscapular brown adipose tissue whole cell lysate at 2.5 µg

Predicted band size: 33 kDa

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody (AB155117)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody (AB155117)

Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue, labeling UCP1 with ab155117 at 1/250 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human UCP1 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

P25874

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Alternative antigen retrieval buffer: Tris-EDTA buffer pH 8.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }

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Product details

What is this antibody validated in?
Anti-UCP1 antibody (ab155117) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse samples.

What is the molecular weight of UCP1?
Anti-UCP1 (ab155117) specifically detects a band for UCP1 (UniProt: P25874) at a molecular weight of 33kDa.

Trusted by the scientific community
Anti-UCP1 (ab155117) was first used in a scientific publication in 2013 and has been cited over 40 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Uncoupling Protein 1 (UCP1) also known as thermogenin is an important protein found in the mitochondria of brown adipose tissue (BAT). It has a molecular mass of approximately 33 kDa. UCP1 functions as a mitochondrial carrier specifically involved in the process of proton transport across the inner mitochondrial membrane. It leads to the dissipation of the proton gradient producing heat instead of ATP. This process is called non-shivering thermogenesis. UCP1 is mainly expressed in brown adipose tissue an area important for heat production in mammals.

Biological function summary

UCP1 plays an essential role in energy metabolism by activating thermogenesis in brown adipocytes. It does not form a larger multi-protein complex but acts as an independent protein facilitating a significant amount of heat production. By uncoupling oxidative phosphorylation UCP1 controls energy expenditure and is central to maintaining body temperature in cold environments. Its function regulates metabolic rate and prevents excess fat accumulation connecting with mechanisms involved in energy balance.

Pathways

UCP1 is an integral component of the metabolic pathway for thermogenesis. The PPAR signaling pathway regulates its expression emphasizing its role in energy homeostasis. Additionally UCP1 interacts with proteins like PGC-1α an important regulator of energy metabolism and mitochondrial biogenesis. This interaction highlights UCP1's involvement in mitochondrial activity and adaptation to changes in temperature or diet linking it to important metabolic functions.

UCP1 has been connected with obesity and metabolic syndrome. Its dysregulation can lead to impaired thermogenic responses and reduced metabolic rate contributing to metabolic disorders. UCP1 is related to leptin a protein that regulates appetite and energy balance which is impacted in cases of obesity. Understanding this relationship helps in exploring potential therapeutic targets for tackling obesity and related metabolic diseases by modulating UCP1 activity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mitochondrial protein responsible for thermogenic respiration, a specialized capacity of brown adipose tissue and beige fat that participates in non-shivering adaptive thermogenesis to temperature and diet variations and more generally to the regulation of energy balance (By similarity). Functions as a long-chain fatty acid/LCFA and proton symporter, simultaneously transporting one LCFA and one proton through the inner mitochondrial membrane (PubMed : 24196960, PubMed : 28781081). However, LCFAs remaining associated with the transporter via their hydrophobic tails, it results in an apparent transport of protons activated by LCFAs. Thereby, dissipates the mitochondrial proton gradient and converts the energy of substrate oxydation into heat instead of ATP. Regulates the production of reactive oxygen species/ROS by mitochondria (By similarity).

See full target information UCP1

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Publications (49)

Recent publications for all applications. Explore the full list and refine your search

Acta physiologica (Oxford, England) 241:e70109 PubMed40990116

2025

Natural Mutation in Naked Mole-Rat UCP1 Refutes Importance of the Histidine Pair Motif for Proton Conductance and Thermogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Michael J Gaudry,Amanda Bundgaard,Maria Kutschke,Klaudia Ostatek,Margeoux A S Dela Rosa,Paul G Crichton,Jane Reznick,Martin Jastroch

The ISME journal 19: PubMed40899693

2025

Convergent gut microbial functional strategies drive energy metabolism adaptation across Ursidae species and challenge the uniqueness of giant panda.

Applications

Unspecified application

Species

Unspecified reactive species

Tingbei Bo,Xiaoming Xu,He Liu,Liqiu Tang,Haihong Xu,Siqi Zhao,Jinzhen Lv,Dehua Wang

Nature communications 15:9652 PubMed39511181

2024

Preoperative methionine restriction induces perivascular adipose tissue browning and improves vein graft remodeling in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Peter Kip,Thijs J Sluiter,Michael R MacArthur,Ming Tao,Nicky Kruit,Sarah J Mitchell,Jonathan Jung,Sander Kooijman,Josh Gorham,Jonathan G Seidman,Paul H A Quax,Julius L Decano,Masanori Aikawa,C Keith Ozaki,James R Mitchell,Margreet R de Vries

Cell metabolism 36:2146-2155.e5 PubMed39084217

2024

Development of a functional beige fat cell line uncovers independent subclasses of cells expressing UCP1 and the futile creatine cycle.

Applications

Unspecified application

Species

Unspecified reactive species

Ariana Vargas-Castillo,Yizhi Sun,Amanda L Smythers,Louisa Grauvogel,Phillip A Dumesic,Margo P Emont,Linus T Tsai,Evan D Rosen,Nathan W Zammit,Sydney M Shaffer,Martha Ordonez,Edward T Chouchani,Steven P Gygi,Tongtong Wang,Anand K Sharma,Miroslav Balaz,Christian Wolfrum,Bruce M Spiegelman

Acta pharmaceutica Sinica. B 14:2137-2152 PubMed38799642

2024

Small molecule conjugates with selective estrogen receptor agonism promote anti-aging benefits in metabolism and skin recovery.

Applications

Unspecified application

Species

Unspecified reactive species

Tarik Zahr,Vijay K Boda,Jian Ge,Lexiang Yu,Zhongzhi Wu,Jianwen Que,Wei Li,Li Qiang

Pharmaceutics 15: PubMed38004519

2023

Selegiline Modulates Lipid Metabolism by Activating AMPK Pathways of Epididymal White Adipose Tissues in HFD-Fed Obese Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hye-Young Joung,Jung-Mi Oh,Min-Suk Song,Young-Bae Kwon,Sungkun Chun

Endocrinology 164: PubMed37944134

2023

Standardized In Vitro Models of Human Adipose Tissue Reveal Metabolic Flexibility in Brown Adipocyte Thermogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Cheryl Cero,Weiguo Shu,Amy L Reese,Diana Douglas,Michael Maddox,Ajeet P Singh,Sahara L Ali,Alexander R Zhu,Jacqueline M Katz,Anne E Pierce,Kelly T Long,Naris Nilubol,Raymond H Cypess,Jonathan L Jacobs,Fang Tian,Aaron M Cypess

Nutrients 15: PubMed37299553

2023

Pecans and Its Polyphenols Prevent Obesity, Hepatic Steatosis and Diabetes by Reducing Dysbiosis, Inflammation, and Increasing Energy Expenditure in Mice Fed a High-Fat Diet.

Applications

Unspecified application

Species

Unspecified reactive species

Claudia Delgadillo-Puga,Ivan Torre-Villalvazo,Lilia G Noriega,Leonardo A Rodríguez-López,Gabriela Alemán,Erik A Torre-Anaya,Yonatan Y Cariño-Cervantes,Berenice Palacios-Gonzalez,Janette Furuzawa-Carballeda,Armando R Tovar,Luis Cisneros-Zevallos

International journal of molecular sciences 24: PubMed37175691

2023

Pods or a Polyphenolic Extract Derived from Them Exert Immunomodulatory, Metabolic, Renoprotective, and Prebiotic Effects in Mice Fed a High-Fat Diet.

Applications

Unspecified application

Species

Unspecified reactive species

Claudia Delgadillo-Puga,Dulce R Sánchez-Castillo,Yonatan Y Cariño-Cervantes,Ivan Torre-Villalvazo,Claudia Tovar-Palacio,Sarai Vásquez-Reyes,Janette Furuzawa-Carballeda,Joshua Ayork Acevedo-Carabantes,María Del Rayo Camacho-Corona,Jorge Luis Guzmán-Mar,Luis Cisneros-Zevallos,Armando R Tovar,Rosa Rebollar-Vega,Georgina Hernández-Montes,Alfredo Ulloa-Aguirre,Berenice Palacios-Gonzalez,Lilia G Noriega

International journal of molecular sciences 24: PubMed36835337

2023

Cardamom ( (L.) Maton) Seeds Intake Increases Energy Expenditure and Reduces Fat Mass in Mice by Modulating Neural Circuits That Regulate Adipose Tissue Lipolysis and Mitochondrial Oxidative Metabolism in Liver and Skeletal Muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Claudia Delgadillo-Puga,Ivan Torre-Villalvazo,Yonatan Y Cariño-Cervantes,Cinthia García-Luna,Paulina Soberanes-Chávez,Patricia de Gortari,Lilia G Noriega,Claudia J Bautista,Luis Cisneros-Zevallos

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-UCP1 antibody

Western blot - Anti-UCP1 antibody (AB155117)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody (AB155117)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (49)

Product promise