Rabbit Polyclonal UCP1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 41 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Mitochondrial brown fat uncoupling protein 1 aa 1-300.
IgG
Rabbit
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
IHC-P | WB | |
---|---|---|
Human | Tested | Expected |
Mouse | Expected | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes Alternative antigen retrieval buffer: Tris-EDTA buffer pH 8. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Select an associated product type
Mitochondrial protein responsible for thermogenic respiration, a specialized capacity of brown adipose tissue and beige fat that participates in non-shivering adaptive thermogenesis to temperature and diet variations and more generally to the regulation of energy balance (By similarity). Functions as a long-chain fatty acid/LCFA and proton symporter, simultaneously transporting one LCFA and one proton through the inner mitochondrial membrane (PubMed:24196960, PubMed:28781081). However, LCFAs remaining associated with the transporter via their hydrophobic tails, it results in an apparent transport of protons activated by LCFAs. Thereby, dissipates the mitochondrial proton gradient and converts the energy of substrate oxydation into heat instead of ATP. Regulates the production of reactive oxygen species/ROS by mitochondria (By similarity).
SLC25A7, UCP, UCP, UCP1, SLC25A7, Mitochondrial brown fat uncoupling protein 1, UCP 1, Solute carrier family 25 member 7, Thermogenin
Rabbit Polyclonal UCP1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 41 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Mitochondrial brown fat uncoupling protein 1 aa 1-300.
IgG
Rabbit
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
Uncoupling Protein 1 (UCP1) also known as thermogenin is an important protein found in the mitochondria of brown adipose tissue (BAT). It has a molecular mass of approximately 33 kDa. UCP1 functions as a mitochondrial carrier specifically involved in the process of proton transport across the inner mitochondrial membrane. It leads to the dissipation of the proton gradient producing heat instead of ATP. This process is called non-shivering thermogenesis. UCP1 is mainly expressed in brown adipose tissue an area important for heat production in mammals.
UCP1 plays an essential role in energy metabolism by activating thermogenesis in brown adipocytes. It does not form a larger multi-protein complex but acts as an independent protein facilitating a significant amount of heat production. By uncoupling oxidative phosphorylation UCP1 controls energy expenditure and is central to maintaining body temperature in cold environments. Its function regulates metabolic rate and prevents excess fat accumulation connecting with mechanisms involved in energy balance.
UCP1 is an integral component of the metabolic pathway for thermogenesis. The PPAR signaling pathway regulates its expression emphasizing its role in energy homeostasis. Additionally UCP1 interacts with proteins like PGC-1α an important regulator of energy metabolism and mitochondrial biogenesis. This interaction highlights UCP1's involvement in mitochondrial activity and adaptation to changes in temperature or diet linking it to important metabolic functions.
UCP1 has been connected with obesity and metabolic syndrome. Its dysregulation can lead to impaired thermogenic responses and reduced metabolic rate contributing to metabolic disorders. UCP1 is related to leptin a protein that regulates appetite and energy balance which is impacted in cases of obesity. Understanding this relationship helps in exploring potential therapeutic targets for tackling obesity and related metabolic diseases by modulating UCP1 activity.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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10% SDS PAGE
All lanes: Western blot - Anti-UCP1 antibody (ab155117) at 1/1000 dilution
All lanes: Mouse interscapular brown adipose tissue whole cell lysate at 2.5 µg
Predicted band size: 33 kDa
Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue, labeling UCP1 with ab155117 at 1/250 dilution.
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