Rabbit Polyclonal UCP1 antibody. Suitable for WB and reacts with Rat, Mouse samples. Cited in 151 publications.
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- Nature communications 14:27542023Endothelial cell-derived stem cell factor promotes lipid accumulation through c-Kit-mediated increase of lipogenic enzymes in brown adipocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyuek Jong Lee,Jueun Lee,Myung Jin Yang,Young-Chan Kim,Seon Pyo Hong,Jung Mo Kim,Geum-Sook Hwang,Gou Young KohPubMed 37179330
- BMC biology 21:272023Cold exposure alters lipid metabolism of skeletal muscle through HIF-1α-induced mitophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWentao Chen,Ziye Xu,Wenjing You,Yanbing Zhou,Liyi Wang,Yuqin Huang,Tizhong ShanPubMed 36750818
- Nature communications 13:72692022Reduced secretion of neuronal growth regulator 1 contributes to impaired adipose-neuronal crosstalk in obesity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElisa Duregotti,Christina M Reumiller,Ursula Mayr,Maria Hasman,Lukas E Schmidt,Sean A Burnap,Konstantinos Theofilatos,Javier Barallobre-Barreiro,Arne Beran,Maria Grandoch,Alessandro Viviano,Marjan Jahangiri,Manuel MayrPubMed 36433953
- The Journal of physiology 601:69-822022An obesogenic diet impairs uncoupled substrate oxidation and promotes whitening of the brown adipose tissue in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel Da Eira,Shailee Jani,Rolando B CeddiaPubMed 36419345
- Nature communications 13:57152022MYPT1-PP1β phosphatase negatively regulates both chromatin landscape and co-activator recruitment for beige adipogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHiroki Takahashi,Ge Yang,Takeshi Yoneshiro,Yohei Abe,Ryo Ito,Chaoran Yang,Junna Nakazono,Mayumi Okamoto-Katsuyama,Aoi Uchida,Makoto Arai,Hitomi Jin,Hyunmi Choi,Myagmar Tumenjargal,Shiyu Xie,Ji Zhang,Hina Sagae,Yanan Zhao,Rei Yamaguchi,Yu Nomura,Yuichi Shimizu,Kaito Yamada,Satoshi Yasuda,Hiroshi Kimura,Toshiya Tanaka,Youichiro Wada,Tatsuhiko Kodama,Hiroyuki Aburatani,Min-Sheng Zhu,Takeshi Inagaki,Timothy F Osborne,Takeshi Kawamura,Yasushi Ishihama,Yoshihiro Matsumura,Juro SakaiPubMed 36175407
- BMC genomics 23:6602022Identification of thermogenesis-related lncRNAs in small extracellular vesicles derived from adipose tissue.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPengyu Hong,Yue Wu,Qi Zhang,Pan Liu,Siyuan Zhang,Mei Yu,Weidong TianPubMed 36117155
- Nature communications 13:51172022Beiging of perivascular adipose tissue regulates its inflammation and vascular remodeling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYusuke Adachi,Kazutaka Ueda,Seitaro Nomura,Kaoru Ito,Manami Katoh,Mikako Katagiri,Shintaro Yamada,Masaki Hashimoto,Bowen Zhai,Genri Numata,Akira Otani,Munetoshi Hinata,Yuta Hiraike,Hironori Waki,Norifumi Takeda,Hiroyuki Morita,Tetsuo Ushiku,Toshimasa Yamauchi,Eiki Takimoto,Issei KomuroPubMed 36071032
- Frontiers in physiology 13:9531992022A mutant mouse shows a sex and diet specific change in fat distribution, reduced food intake and depot-specific upregulation of WAT browning.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMilan Mušo,Liz Bentley,Lucie Vizor,Marianne Yon,Keith Burling,Peter Barker,Louisa A K Zolkiewski,Roger D Cox,Rebecca DumbellPubMed 36091365
- Frontiers in physiology 13:8821512022BAIBA Involves in Hypoxic Training Induced Browning of White Adipose Tissue in Obese Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunpeng Feng,Xuebing Wang,Yingli Lu,Chang Yu,Xinyan Wang,Lianshi FengPubMed 35832480
- Cells 11:2022COX-2 Deficiency Promotes White Adipogenesis via PGE2-Mediated Paracrine Mechanism and Exacerbates Diet-Induced Obesity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChunqing Wang,Xing Zhang,Liping Luo,Yan Luo,Dandan Wu,Dianna Spilca,Que Le,Xin Yang,Katelyn Alvarez,William Curtis Hines,Xuexian O Yang,Meilian LiuPubMed 35681514
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | |
|---|---|
| Human | Predicted |
| Mouse | Tested |
| Rat | Tested |
| Chimpanzee | Predicted |
| Dog | Predicted |
| Hamster | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1 µg/mL | Notes - |
Species Mouse | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Hamster, Dog, Human, Chimpanzee | Dilution info - | Notes - |
Associated Products
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Target data
Function
Mitochondrial protein responsible for thermogenic respiration, a specialized capacity of brown adipose tissue and beige fat that participates in non-shivering adaptive thermogenesis to temperature and diet variations and more generally to the regulation of energy balance (By similarity). Functions as a long-chain fatty acid/LCFA and proton symporter, simultaneously transporting one LCFA and one proton through the inner mitochondrial membrane (PubMed:24196960, PubMed:28781081). However, LCFAs remaining associated with the transporter via their hydrophobic tails, it results in an apparent transport of protons activated by LCFAs. Thereby, dissipates the mitochondrial proton gradient and converts the energy of substrate oxydation into heat instead of ATP. Regulates the production of reactive oxygen species/ROS by mitochondria (By similarity).
Alternative names
SLC25A7, UCP, UCP1, Mitochondrial brown fat uncoupling protein 1, UCP 1, Solute carrier family 25 member 7, Thermogenin
Recommended products
Rabbit Polyclonal UCP1 antibody. Suitable for WB and reacts with Rat, Mouse samples. Cited in 151 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Uncoupling Protein 1 (UCP1) also known as thermogenin is an important protein found in the mitochondria of brown adipose tissue (BAT). It has a molecular mass of approximately 33 kDa. UCP1 functions as a mitochondrial carrier specifically involved in the process of proton transport across the inner mitochondrial membrane. It leads to the dissipation of the proton gradient producing heat instead of ATP. This process is called non-shivering thermogenesis. UCP1 is mainly expressed in brown adipose tissue an area important for heat production in mammals.
- Biological function summary
UCP1 plays an essential role in energy metabolism by activating thermogenesis in brown adipocytes. It does not form a larger multi-protein complex but acts as an independent protein facilitating a significant amount of heat production. By uncoupling oxidative phosphorylation UCP1 controls energy expenditure and is central to maintaining body temperature in cold environments. Its function regulates metabolic rate and prevents excess fat accumulation connecting with mechanisms involved in energy balance.
- Pathways
UCP1 is an integral component of the metabolic pathway for thermogenesis. The PPAR signaling pathway regulates its expression emphasizing its role in energy homeostasis. Additionally UCP1 interacts with proteins like PGC-1α an important regulator of energy metabolism and mitochondrial biogenesis. This interaction highlights UCP1's involvement in mitochondrial activity and adaptation to changes in temperature or diet linking it to important metabolic functions.
- Associated diseases and disorders
UCP1 has been connected with obesity and metabolic syndrome. Its dysregulation can lead to impaired thermogenic responses and reduced metabolic rate contributing to metabolic disorders. UCP1 is related to leptin a protein that regulates appetite and energy balance which is impacted in cases of obesity. Understanding this relationship helps in exploring potential therapeutic targets for tackling obesity and related metabolic diseases by modulating UCP1 activity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-UCP1 antibody (ab23841)
All lanes: Western blot - Anti-UCP1 antibody (ab23841) at 1 µg/mL
Lane 1: Adult Mouse Brown Adipose Tissue Lysate at 20 µg
Lane 2: Adult Rat Brown Adipose Tissue Lysate at 20 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 100 kDa, 33 kDa, 50 kDa
Exposure time: 30s
Western blot - Anti-UCP1 antibody (ab23841)
Western blot: Anti-UCP1 antibody (ab23841) staining at 1 ug/ml, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab23841 was shown to bind specifically to UCP1. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-UCP1 antibody (ab23841) at 1 µg/mL
Lane 1: Mouse brown adipose cell lysate at 20 µg
Lane 2: Mouse white adipose cell lysate at 20 µg
Lane 3: Rat brown adipose cell lysate at 20 µg
Lane 4: Rat white adipose cell lysate at 20 µg
SecondaryAll lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
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