Anti-UCP1 antibody

• WB

Lab

Western blot - Anti-UCP1 antibody (AB23841)

Western blot : Anti-UCP1 antibody (ab23841) staining at 1 ug/ml, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab23841 was shown to bind specifically to UCP1. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-UCP1 antibody (ab23841) at 1 µg/mL

Lane 1:

Mouse brown adipose cell lysate at 20 µg

Lane 2:

Mouse white adipose cell lysate at 20 µg

Lane 3:

Rat brown adipose cell lysate at 20 µg

Lane 4:

Rat white adipose cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

false

• WB

Ap

Western blot - Anti-UCP1 antibody (AB23841)

All lanes:

Western blot - Anti-UCP1 antibody (ab23841) at 1 µg/mL

Lane 1:

Adult Mouse Brown Adipose Tissue Lysate at 20 µg

Lane 2:

Adult Rat Brown Adipose Tissue Lysate at 20 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 33 kDa

Observed band size: 100 kDa,33 kDa,50 kDa

false

Exposure time: 30s