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AB209483

Anti-UCP1 antibody [EPR20381]

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(4 Reviews)

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(56 Publications)

Anti-UCP1 antibody [EPR20381] (ab209483) is a rabbit monoclonal antibody detecting UCP1 in Western Blot, IP, IHC-P. Suitable for Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 30 publications

View Alternative Names

Slc25a7, Ucp, Ucp1, Mitochondrial brown fat uncoupling protein 1, UCP 1, Solute carrier family 25 member 7, Thermogenin

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody [EPR20381] (AB209483)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody [EPR20381] (AB209483)

Immunohistochemical analysis of paraffin-embedded rat brown adipose tissue labeling UCP1 with ab209483 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on rat brown adipose tissue is observed [PMID : 24753268] [PMID : 23824424].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody [EPR20381] (AB209483)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody [EPR20381] (AB209483)

Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue and white adipose tissue labeling UCP1 with ab209483 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on mouse brown adipose tissue, no staining on adjacent white adipose tissue [PMID : 24753268] [PMID : 23824424].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody [EPR20381] (AB209483)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody [EPR20381] (AB209483)

Immunohistochemical analysis of paraffin-embedded rat brown adipose tissue and white adipose tissue labeling UCP1 with ab209483 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on rat brown adipose tissue, no staining on adjacent white adipose tissue [PMID : 24753268] [PMID : 23824424].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody [EPR20381] (AB209483)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody [EPR20381] (AB209483)

Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue labeling UCP1 with ab209483 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on mouse brown adipose tissue is observed [PMID : 24753268] [PMID : 23824424].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-UCP1 antibody [EPR20381] (AB209483)
  • IP

Supplier Data

Immunoprecipitation - Anti-UCP1 antibody [EPR20381] (AB209483)

UCP1 was immunoprecipitated from 0.35 mg of rat brown adipose lysate with ab209483 at 1/30 dilution.

Western blot was performed from the immunoprecipitate using ab209483 at 1/5000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Rat brown adipose lysate, 10 ug (Input).

Lane 2 : ab209483 IP in rat brown adipose lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab209483 in rat brown adipose lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 5 seconds.

All lanes:

Immunoprecipitation - Anti-UCP1 antibody [EPR20381] (ab209483)

Predicted band size: 33 kDa

Observed band size: 33 kDa

false

Western blot - Anti-UCP1 antibody [EPR20381] (AB209483)
  • WB

Supplier Data

Western blot - Anti-UCP1 antibody [EPR20381] (AB209483)

Blocking/Dilution buffer : 5% NFDM/TBST.

UCP1 is a specific marker for brown adipose tissue (BAT). It is expressed exclusively in BAT, but not in white adipose tissue.

Negative control : Mouse white adipose tissue. (PMID : 2999153).

All lanes:

Western blot - Anti-UCP1 antibody [EPR20381] (ab209483) at 1/5000 dilution

Lane 1:

Mouse brown adipose tissue lysate at 20 µg

Lane 2:

Mouse white adipose tissue lysate at 20 µg

Lane 3:

Rat brown adipose tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 33 kDa

Observed band size: 33 kDa

false

Exposure time: 3s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20381

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

WB, IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Product details

What is this antibody validated in?
Anti-UCP1 antibody [EPR20381] (ab209483) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Mouse, Rat samples.

What is the molecular weight of UCP1?
Anti-UCP1 [EPR20381] (ab209483) specifically detects a band for UCP1 (UniProt: P12242) at a molecular weight of 33kDa.

Trusted by the scientific community
Anti-UCP1 [EPR20381] (ab209483) was first used in a scientific publication in 2017 and has been cited over 30 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR20381] also available for your convenience: ab209483, Carrier free - ab222397, Alexa Fluor® 647 - ab225489, Alexa Fluor® 488 - ab225490, Alexa Fluor® 594 - ab311798, Alexa Fluor® 568 - ab313081, Alexa Fluor® 555 - ab313275, Alexa Fluor® 750 - ab321365

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Uncoupling Protein 1 (UCP1) also known as thermogenin is an important protein found in the mitochondria of brown adipose tissue (BAT). It has a molecular mass of approximately 33 kDa. UCP1 functions as a mitochondrial carrier specifically involved in the process of proton transport across the inner mitochondrial membrane. It leads to the dissipation of the proton gradient producing heat instead of ATP. This process is called non-shivering thermogenesis. UCP1 is mainly expressed in brown adipose tissue an area important for heat production in mammals.
Biological function summary

UCP1 plays an essential role in energy metabolism by activating thermogenesis in brown adipocytes. It does not form a larger multi-protein complex but acts as an independent protein facilitating a significant amount of heat production. By uncoupling oxidative phosphorylation UCP1 controls energy expenditure and is central to maintaining body temperature in cold environments. Its function regulates metabolic rate and prevents excess fat accumulation connecting with mechanisms involved in energy balance.

Pathways

UCP1 is an integral component of the metabolic pathway for thermogenesis. The PPAR signaling pathway regulates its expression emphasizing its role in energy homeostasis. Additionally UCP1 interacts with proteins like PGC-1α an important regulator of energy metabolism and mitochondrial biogenesis. This interaction highlights UCP1's involvement in mitochondrial activity and adaptation to changes in temperature or diet linking it to important metabolic functions.

UCP1 has been connected with obesity and metabolic syndrome. Its dysregulation can lead to impaired thermogenic responses and reduced metabolic rate contributing to metabolic disorders. UCP1 is related to leptin a protein that regulates appetite and energy balance which is impacted in cases of obesity. Understanding this relationship helps in exploring potential therapeutic targets for tackling obesity and related metabolic diseases by modulating UCP1 activity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mitochondrial transporter that functions as a long-chain fatty acid/LCFA and proton symporter, simultaneously transporting one LCFA and one proton through the inner mitochondrial membrane. However, LCFAs remaining associated with the transporter via their hydrophobic tails, it results in an apparent transport of protons activated by LCFAs. Thereby, dissipates the mitochondrial proton gradient and converts the energy of substrate oxydation into heat instead of ATP (PubMed : 23063128). Responsible for thermogenic respiration, a specialized capacity of brown adipose tissue and beige fat that participates in non-shivering adaptive thermogenesis to temperature and diet variations and more generally to the regulation of energy balance (PubMed : 19187776, PubMed : 23063128, PubMed : 27027295, PubMed : 9139827). Regulates the production of reactive oxygen species/ROS by mitochondria (PubMed : 20416274, PubMed : 20466728).
See full target information Ucp1
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Publications (56)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:7414 PubMed40789858

2025

Adipocyte RNA-binding protein CELF1 promotes beiging of white fat through stabilizing Dio2 mRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Zeng,Liuling Xiao,Jiajie Li,Han Wu,Xiaolong Guo,Fukang Zhu,Xinyu Yu,Yewei Cui,Xueya Zhao,Yumeng Wang,Ting Zhang,Weijiong He,Hongxiang Zeng,Xi Li

eLife 14: PubMed40587199

2025

Tissue-specific responses to TFAM and mtDNA copy number manipulation in prematurely ageing mice.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Sophie Kremer,Guanbin Gao,Giovanni Rigoni,Roberta Filograna,Mara Mennuni,Rolf Wibom,Ákos Végvári,Camilla Koolmeister,Nils-Göran Larsson

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e05545 PubMed40548473

2025

The Long Noncoding RNA ΒFaar Promotes White Adipose Tissue Browning and Prevents Diet-Induced Obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Yang,Bin Huang,Baixue Sha,Danni Gao,Yimeng Qin,Ziyi Li,Xi Chen,Yinuo Jin,Yi Pan,Yanfeng Zhang,Yumeng Shen,Yu Liu,Liang Jin,Fangfang Zhang

International journal of molecular sciences 26: PubMed40362353

2025

The Regulator of G Protein Signaling 14 Knockout Mouse, a Model of Healthful Longevity Protects Against Obesity and Glucose Intolerance Through a Brown Adipose Tissue Mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Stephen F Vatner,Jie Zhang,Marko Oydanich,Dorothy E Vatner

iScience 28:112038 PubMed40124497

2025

deficiency in neutrophils alleviates symptoms induced by high-fat diet.

Applications

Unspecified application

Species

Unspecified reactive species

Brinja Leinweber,Violetta Pilorz,Iwona Olejniczak,Ludmila Skrum,Kimberly Begemann,Isabel Heyde,Sarah Stenger,Christian David Sadik,Henrik Oster

BMC microbiology 25:135 PubMed40075266

2025

Fecal microbiota transplantation mitigates postdieting weight regain in mice by modulating the gut-liver axis.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Cao,Jiangwei Xu,Han Wang,Wanya Yi,Dandan Yang,Ju Yang,Jing Sun,Yingyu Wang,Feng Zhang,Jiai Yan,Dan Li

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2416598 PubMed40051328

2025

MTCH2 Suppresses Thermogenesis by Regulating Autophagy in Adipose Tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Yuan Zhao,Ben-Chi Zhao,Hui-Lin Li,Ying Liu,Bei Wang,An-Qi Li,Tian-Shu Zeng,Hannah Xiaoyan Hui,Jia Sun,Domagoj Cikes,Nele Gheldof,Jorg Hager,Jian-Xun Mi,D Ross Laybutt,Yin-Yue Deng,Yan-Chuan Shi,G Gregory Neely,Qiao-Ping Wang

Experimental physiology 110:1099-1113 PubMed39721028

2024

Housing temperature influences exercise-induced glucose regulation and expression of exerkines in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zhijian Rao,Xue Geng,Peng Huang,Qiangman Wei,Shijie Liu,Chaoyi Qu,Jiexiu Zhao

Obesity (Silver Spring, Md.) 32:2321-2333 PubMed39540259

2024

Attenuation of high-fat diet-induced weight gain by apolipoprotein A4.

Applications

Unspecified application

Species

Unspecified reactive species

Hsuan-Chih N Kuo,Zachary LaRussa,Flora Mengyang Xu,Leslie A Consitt,Min Liu,W Sean Davidson,Vishwajeet Puri,Karen T Coschigano,Haifei Shi,Chunmin C Lo

Molecular nutrition & food research 68:e2400451 PubMed39520336

2024

Intermittent Fasting Improves Insulin Resistance by Modulating the Gut Microbiota and Bile Acid Metabolism in Diet-Induced Obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Sha Lei,Guanghui Liu,Shouli Wang,Guannan Zong,Xiaoya Zhang,Lingling Pan,Junfeng Han
View all publications
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Product promise

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