AB314239

Anti-UCP1 antibody [RM1066]

BOND RX™ Validated
20ul selling size
RabMAb
Recombinant
KO Validated
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Rabbit Recombinant Multiclonal UCP1 antibody. Suitable for WB, IHC-P, IP and reacts with Mouse, Rat samples.

View Alternative Names

Slc25a7, Ucp, Ucp1, Mitochondrial brown fat uncoupling protein 1, UCP 1, Solute carrier family 25 member 7, Thermogenin

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody [RM1066] (AB314239)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody [RM1066] (AB314239)

Immunohistochemical analysis of paraffin-embedded Mouse white adipose tissue labeling UCP1 with ab314239 at 1/5000 (0.101 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on mouse white adipose.The section was incubated with ab314239 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody [RM1066] (AB314239)

Immunohistochemical analysis of paraffin-embedded (A) Brown fat tissue from wild-type C57BL/6JGpt mice / (B) Brown fat tissue from UCP1 knockout mice tissue labeling UCP1 with ab314239 at 1/5000 ilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) Brown fat tissue from wild-type C57BL/6JGpt mice and no staining on (B) Brown fat tissue from UCP1 knockout mice.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument

The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and UCP1-KO homozygous mice (Strain ID : T037633).

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody [RM1066] (AB314239)

Immunohistochemical analysis of paraffin-embedded Rat white adipose tissue labeling UCP1 with ab314239 at 1/5000 (0.101 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on rat white adipose.The section was incubated with ab314239 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody [RM1066] (AB314239)

Immunohistochemical analysis of paraffin-embedded Rat brown adipose tissue labeling UCP1 with ab314239 at 1/5000 (0.101 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat brown adipose.The section was incubated with ab314239 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody [RM1066] (AB314239)

Immunohistochemical analysis of paraffin-embedded Mouse brown adipose tissue labeling UCP1 with ab314239 at 1/5000 (0.101 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse brown adipose.The section was incubated with ab314239 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Supplier Data

Immunoprecipitation - Anti-UCP1 antibody [RM1066] (AB314239)

UCP1 was immunoprecipitated from 0.35 mg Rat brown fat tissue lysate with ab314239 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314239 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Rat brown fat tissue lysate Lane 2 : ab314239 IP in Rat brown fat tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab314239 in rat brown fat tissue lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-UCP1 antibody [RM1066] (ab314239) at 1/30 dilution

All lanes:

Rat brown fat tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 8s

Supplier Data

Immunoprecipitation - Anti-UCP1 antibody [RM1066] (AB314239)

UCP1 was immunoprecipitated from 0.35 mg Mouse brown fat tissue lysate with ab314239 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314239 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Mouse brown fat tissue lysate Lane 2 : ab314239 IP in Mouse brown fat tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab314239 in mouse brown fat tissue lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-UCP1 antibody [RM1066] (ab314239) at 1/30 dilution

All lanes:

Mouse brown fat tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 8s

Supplier Data

Western blot - Anti-UCP1 antibody [RM1066] (AB314239)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : White adipose tissue(PMID : 2999153). In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-UCP1 antibody [RM1066] (ab314239) at 1/1000 dilution

Lane 1:

Mouse brown fat tissue lysate at 20 µg

Lane 2:

Mouse white fat tissue lysate at 20 µg

Lane 3:

Rat brown fat tissue lysate at 20 µg

Lane 4:

Rat white fat tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 33 kDa

false

Exposure time: 6s

Lab

Western blot - Anti-UCP1 antibody [RM1066] (AB314239)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and UCP1-KO homozygous mice (Strain ID : T037633).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) was used at 1/200000 (36KDa).

All lanes:

Western blot - Anti-UCP1 antibody [RM1066] (ab314239) at 1/1000 dilution

Lane 1:

Wild-type mouse brown fat tissue lysate (female) at 20 µg

Lane 2:

UCP1 knockout mouse brown fat tissue lysate (female) at 20 µg

Lane 3:

Wild-type mouse brown fat tissue lysate (male) at 20 µg

Lane 4:

UCP1 knockout mouse brown fat tissue lysate (male) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 33 kDa,36 kDa

false

Exposure time: 4s

Carrier free

Anti-UCP1 antibody [RM1066] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RM1066

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IHC-P, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

View our range of recombinant multiclonal antibodies.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Uncoupling Protein 1 (UCP1) also known as thermogenin is an important protein found in the mitochondria of brown adipose tissue (BAT). It has a molecular mass of approximately 33 kDa. UCP1 functions as a mitochondrial carrier specifically involved in the process of proton transport across the inner mitochondrial membrane. It leads to the dissipation of the proton gradient producing heat instead of ATP. This process is called non-shivering thermogenesis. UCP1 is mainly expressed in brown adipose tissue an area important for heat production in mammals.

Biological function summary

UCP1 plays an essential role in energy metabolism by activating thermogenesis in brown adipocytes. It does not form a larger multi-protein complex but acts as an independent protein facilitating a significant amount of heat production. By uncoupling oxidative phosphorylation UCP1 controls energy expenditure and is central to maintaining body temperature in cold environments. Its function regulates metabolic rate and prevents excess fat accumulation connecting with mechanisms involved in energy balance.

Pathways

UCP1 is an integral component of the metabolic pathway for thermogenesis. The PPAR signaling pathway regulates its expression emphasizing its role in energy homeostasis. Additionally UCP1 interacts with proteins like PGC-1α an important regulator of energy metabolism and mitochondrial biogenesis. This interaction highlights UCP1's involvement in mitochondrial activity and adaptation to changes in temperature or diet linking it to important metabolic functions.

UCP1 has been connected with obesity and metabolic syndrome. Its dysregulation can lead to impaired thermogenic responses and reduced metabolic rate contributing to metabolic disorders. UCP1 is related to leptin a protein that regulates appetite and energy balance which is impacted in cases of obesity. Understanding this relationship helps in exploring potential therapeutic targets for tackling obesity and related metabolic diseases by modulating UCP1 activity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mitochondrial transporter that functions as a long-chain fatty acid/LCFA and proton symporter, simultaneously transporting one LCFA and one proton through the inner mitochondrial membrane. However, LCFAs remaining associated with the transporter via their hydrophobic tails, it results in an apparent transport of protons activated by LCFAs. Thereby, dissipates the mitochondrial proton gradient and converts the energy of substrate oxydation into heat instead of ATP (PubMed : 23063128). Responsible for thermogenic respiration, a specialized capacity of brown adipose tissue and beige fat that participates in non-shivering adaptive thermogenesis to temperature and diet variations and more generally to the regulation of energy balance (PubMed : 19187776, PubMed : 23063128, PubMed : 27027295, PubMed : 9139827). Regulates the production of reactive oxygen species/ROS by mitochondria (PubMed : 20416274, PubMed : 20466728).

See full target information Ucp1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-UCP1 antibody [RM1066]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody [RM1066] (AB314239)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody [RM1066] (AB314239)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody [RM1066] (AB314239)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody [RM1066] (AB314239)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody [RM1066] (AB314239)

Immunoprecipitation - Anti-UCP1 antibody [RM1066] (AB314239)

Immunoprecipitation - Anti-UCP1 antibody [RM1066] (AB314239)

Western blot - Anti-UCP1 antibody [RM1066] (AB314239)

Western blot - Anti-UCP1 antibody [RM1066] (AB314239)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Reagents, Controls & Accessories

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise