Rabbit Polyclonal UCP2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 30 publications.
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- Journal of hepatocellular carcinoma 9:1229-12462022The Abnormal Proliferation of Hepatocytes is Associated with MC-LR and C-Terminal Truncated HBX Synergistic Disturbance of the Redox Balance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDong-Mei Cai,Fan-Biao Mei,Chao-Jun Zhang,San-Chun An,Rui-Bo Lv,Guan-Hua Ren,Chan-Chan Xiao,Long Long,Tian-Ren Huang,Wei DengPubMed 36505941
- Neuroscience bulletin 38:1347-13642022Anti-Seizure and Neuronal Protective Effects of Irisin in Kainic Acid-Induced Chronic Epilepsy Model with Spontaneous Seizures.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Yu,Yao Cheng,Yaru Cui,Yujie Zhai,Wenshen Zhang,Mengdi Zhang,Wenyu Xin,Jia Liang,Xiaohong Pan,Qiaoyun Wang,Hongliu SunPubMed 35821335
- International journal of molecular sciences 23:2022Effects of Endurance Training on the Coenzyme Q Redox State in Rat Heart, Liver, and Brain at the Tissue and Mitochondrial Levels: Implications for Reactive Oxygen Species Formation and Respiratory Chain Remodeling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKarolina Dominiak,Lukasz Galganski,Adrianna Budzinska,Andrzej Woyda-Ploszczyca,Jerzy A Zoladz,Wieslawa JarmuszkiewiczPubMed 35055078
- Molecular medicine reports 25:2021Phytochemical‑rich herbal formula ATG‑125 protects against sucrose‑induced gastrocnemius muscle atrophy by rescuing Akt signaling and improving mitochondrial dysfunction in young adult mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChing-Chuan Yeh,Hsuan-Miao Liu,Ming-Chung Lee,Yann-Lii Leu,Wei-Han Chiang,Hen-Hong Chang,Tzung-Yan LeePubMed 34913071
- Frontiers in cellular neuroscience 15:7385332021Neuroprotective Effects of Exogenous Irisin in Kainic Acid-Induced Status Epilepticus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYao Cheng,Yaru Cui,Yujie Zhai,Wenyu Xin,Yan Yu,Jia Liang,Shucui Li,Hongliu SunPubMed 34658794
- International journal of molecular sciences 22:2021Losartan Prevents Hepatic Steatosis and Macrophage Polarization by Inhibiting HIF-1α in a Murine Model of NAFLD.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCheng-Hui Wang,Hsuan-Miao Liu,Zi-Yu Chang,Tse-Hung Huang,Tzung-Yan LeePubMed 34360607
- Cancer chemotherapy and pharmacology 88:633-6422021UCP-2 inhibitor enhanced the efficacy of trastuzumab against HER2 positive breast cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Hua,Zhe Zhang,Lili Zhang,Yan Sun,Yuan YuanPubMed 34146128
- Annals of translational medicine 9:2592021The effects of UCP2 on autophagy through the AMPK signaling pathway in septic cardiomyopathy and the underlying mechanism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia-Yu Mao,Long-Xiang Su,Dong-Kai Li,Hong-Min Zhang,Xiao-Ting Wang,Da-Wei LiuPubMed 33708886
- Journal of diabetes research 2020:70195972020Regulation and Mechanism of miR-518d through the PPAR-Mediated NF-B Pathway in the Development of Gestational Diabetes Mellitus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Qiu et. al.PubMed 33123597
- Journal of cellular physiology 236:931-9452020Involvement of GPX4 in irisin's protection against ischemia reperfusion-induced acute kidney injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia Zhang,Jianbin Bi,Yifan Ren,Zhaoqing Du,Teng Li,Tao Wang,Lin Zhang,Mengzhou Wang,Shasha Wei,Yi Lv,Rongqian WuPubMed 32583428
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cat | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Hamster | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Pig | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Hamster, Cat, Dog, Pig, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Hamster, Cat, Dog, Pig, Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Antiporter that exports dicarboxylate intermediates of the Krebs cycle in exchange for phosphate plus a proton across the inner membrane of mitochondria, a process driven by mitochondrial motive force with an overall impact on glycolysis, glutaminolysis and glutathione-dependent redox balance. Continuous export of oxaloacetate and related four-carbon dicarboxylates from mitochondrial matrix into the cytosol negatively regulates the oxidation of acetyl-CoA substrates via the Krebs cycle, lowering the ATP/ADP ratio and reactive oxygen species (ROS) production (PubMed:24395786). May mediate inducible proton entry into the mitochondrial matrix affecting ATP turnover as a protection mechanism against oxidative stress. The proton currents are most likely associated with fatty acid flipping across the inner membrane of mitochondria in a metabolic process regulated by free fatty acids and purine nucleotides (By similarity) (PubMed:11171965, PubMed:11278935, PubMed:22524567, PubMed:26182433, PubMed:33373220). Regulates the use of glucose as a source of energy. Required for glucose-induced DRP1-dependent mitochondrial fission and neuron activation in the ventromedial nucleus of the hypothalamus (VMH). This mitochondrial adaptation mechanism modulates the VMH pool of glucose-excited neurons with an impact on systemic glucose homeostasis (By similarity). Regulates ROS levels and metabolic reprogramming of macrophages during the resolution phase of inflammation. Attenuates ROS production in response to IL33 to preserve the integrity of the Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages (By similarity). Can unidirectionally transport anions including L-malate, L-aspartate, phosphate and chloride ions (PubMed:22524567, PubMed:24395786, PubMed:26182433). Does not mediate adaptive thermogenesis (By similarity).
Alternative names
SLC25A8, UCP2, Dicarboxylate carrier SLC25A8, Mitochondrial uncoupling protein 2, Solute carrier family 25 member 8, UCPH, UCP 2
Recommended products
Rabbit Polyclonal UCP2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 30 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
UCP2 also known as Uncoupling Protein 2 is a mitochondrial protein that plays a role in regulating the production of heat by uncoupling oxidative phosphorylation from ATP synthesis. It has a molecular mass of approximately 33 kDa. UCP2 can be found in various tissues like the brain pancreas heart and adipose tissue. This protein facilitates the transport of protons across the inner mitochondrial membrane which dissipates the proton gradient leading to a reduction in ATP synthesis efficiency.
- Biological function summary
UCP2 influences energy balance and metabolism in cells. It is not part of a complex but it functions independently as a transporter within the mitochondrial membrane. UCP2 modulates reactive oxygen species (ROS) production impacting antioxidant defenses and influencing insulin secretion. Its role in decreasing ROS is particularly important for protecting cells from oxidative damage and helping in the maintenance of cellular homeostasis.
- Pathways
UCP2 is critical to thermogenesis and energy expenditure mechanisms. It is involved in the oxidative phosphorylation pathway affecting how cells produce energy. The protein is related to others in its family such as UCP1 and UCP3 which have similar functions but are expressed differently. UCP2 also intersects with pathways involving glucose metabolism where it influences insulin function and glucose uptake indirectly through its regulatory action.
- Associated diseases and disorders
UCP2 has links to obesity and diabetes. Its role in energy regulation and control of ROS offers insights into how imbalances can lead to metabolic syndromes. In obesity altered UCP2 expression may contribute to changes in energy expenditure and fat storage regulation. For diabetes UCP2 is closely connected to insulin signaling where its function impacts insulin secretion from pancreatic beta-cells. These connections underline the potential of UCP2 as a therapeutic target for metabolic diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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2 product images
Western blot - Anti-UCP2 antibody (ab97931)
All lanes: Western blot - Anti-UCP2 antibody (ab97931) at 1 µg/mL
Lane 1: Human adipose normal tissue lysate - total protein (ab28980) at 10 µg
Lane 2: Human spleen tissue lysate - total protein (ab29699) at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 100 kDa, 30 kDa
Exposure time: 3min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP2 antibody (ab97931)
IHC image of ab97931 staining in human lymph node formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab97931, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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