AB97931

Anti-UCP2 antibody

Name: Anti-UCP2 antibody (ab97931) | Abcam
Brand: Abcam Limited
SKU: AB97931
Rating: 2 (5 reviews)

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What is this?

(5 Reviews)

(33 Publications)

Rabbit Polyclonal UCP2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 33 publications.

View Alternative Names

SLC25A8, UCP2, Dicarboxylate carrier SLC25A8, Mitochondrial uncoupling protein 2, Solute carrier family 25 member 8, UCPH, UCP 2

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP2 antibody (AB97931)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP2 antibody (AB97931)

IHC image of ab97931 staining in human lymph node formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab97931, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Unknown

Western blot - Anti-UCP2 antibody (AB97931)

All lanes:

Western blot - Anti-UCP2 antibody (ab97931) at 1 µg/mL

Lane 1:

Human adipose normal tissue lysate - total protein (<a href='/en-us/products/unavailable/human-adipose-normal-tissue-lysate-total-protein-ab28980'>ab28980</a>) at 10 µg

Lane 2:

Human spleen tissue lysate - total protein (<a href='/en-us/products/unavailable/human-spleen-tissue-lysate-total-protein-ab29699'>ab29699</a>) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 33 kDa

Observed band size: 100 kDa,30 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UCP2 also known as Uncoupling Protein 2 is a mitochondrial protein that plays a role in regulating the production of heat by uncoupling oxidative phosphorylation from ATP synthesis. It has a molecular mass of approximately 33 kDa. UCP2 can be found in various tissues like the brain pancreas heart and adipose tissue. This protein facilitates the transport of protons across the inner mitochondrial membrane which dissipates the proton gradient leading to a reduction in ATP synthesis efficiency.

Biological function summary

UCP2 influences energy balance and metabolism in cells. It is not part of a complex but it functions independently as a transporter within the mitochondrial membrane. UCP2 modulates reactive oxygen species (ROS) production impacting antioxidant defenses and influencing insulin secretion. Its role in decreasing ROS is particularly important for protecting cells from oxidative damage and helping in the maintenance of cellular homeostasis.

Pathways

UCP2 is critical to thermogenesis and energy expenditure mechanisms. It is involved in the oxidative phosphorylation pathway affecting how cells produce energy. The protein is related to others in its family such as UCP1 and UCP3 which have similar functions but are expressed differently. UCP2 also intersects with pathways involving glucose metabolism where it influences insulin function and glucose uptake indirectly through its regulatory action.

UCP2 has links to obesity and diabetes. Its role in energy regulation and control of ROS offers insights into how imbalances can lead to metabolic syndromes. In obesity altered UCP2 expression may contribute to changes in energy expenditure and fat storage regulation. For diabetes UCP2 is closely connected to insulin signaling where its function impacts insulin secretion from pancreatic beta-cells. These connections underline the potential of UCP2 as a therapeutic target for metabolic diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Antiporter that exports dicarboxylate intermediates of the Krebs cycle in exchange for phosphate plus a proton across the inner membrane of mitochondria, a process driven by mitochondrial motive force with an overall impact on glycolysis, glutaminolysis and glutathione-dependent redox balance. Continuous export of oxaloacetate and related four-carbon dicarboxylates from mitochondrial matrix into the cytosol negatively regulates the oxidation of acetyl-CoA substrates via the Krebs cycle, lowering the ATP/ADP ratio and reactive oxygen species (ROS) production (PubMed : 24395786). May mediate inducible proton entry into the mitochondrial matrix affecting ATP turnover as a protection mechanism against oxidative stress. The proton currents are most likely associated with fatty acid flipping across the inner membrane of mitochondria in a metabolic process regulated by free fatty acids and purine nucleotides (By similarity) (PubMed : 11171965, PubMed : 11278935, PubMed : 22524567, PubMed : 26182433, PubMed : 33373220). Regulates the use of glucose as a source of energy. Required for glucose-induced DRP1-dependent mitochondrial fission and neuron activation in the ventromedial nucleus of the hypothalamus (VMH). This mitochondrial adaptation mechanism modulates the VMH pool of glucose-excited neurons with an impact on systemic glucose homeostasis (By similarity). Regulates ROS levels and metabolic reprogramming of macrophages during the resolution phase of inflammation. Attenuates ROS production in response to IL33 to preserve the integrity of the Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages (By similarity). Can unidirectionally transport anions including L-malate, L-aspartate, phosphate and chloride ions (PubMed : 22524567, PubMed : 24395786, PubMed : 26182433). Does not mediate adaptive thermogenesis (By similarity).

See full target information UCP2

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Publications (33)

Recent publications for all applications. Explore the full list and refine your search

Investigative ophthalmology & visual science 65:14 PubMed38175638

2024

UCP2-SIRT3 Signaling Relieved Hyperglycemia-Induced Oxidative Stress and Senescence in Diabetic Retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Shenping Li,Dandan Sun,Shimei Chen,Shuchang Zhang,Qing Gu,Yinchen Shen,Li Xu,Xun Xu,Fang Wei,Ning Wang

Journal of hepatocellular carcinoma 9:1229-1246 PubMed36505941

2022

The Abnormal Proliferation of Hepatocytes is Associated with MC-LR and C-Terminal Truncated HBX Synergistic Disturbance of the Redox Balance.

Applications

Unspecified application

Species

Unspecified reactive species

Dong-Mei Cai,Fan-Biao Mei,Chao-Jun Zhang,San-Chun An,Rui-Bo Lv,Guan-Hua Ren,Chan-Chan Xiao,Long Long,Tian-Ren Huang,Wei Deng

Neuroscience bulletin 38:1347-1364 PubMed35821335

2022

Anti-Seizure and Neuronal Protective Effects of Irisin in Kainic Acid-Induced Chronic Epilepsy Model with Spontaneous Seizures.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Yu,Yao Cheng,Yaru Cui,Yujie Zhai,Wenshen Zhang,Mengdi Zhang,Wenyu Xin,Jia Liang,Xiaohong Pan,Qiaoyun Wang,Hongliu Sun

Molecular medicine reports 25: PubMed35211764

2022

lncRNA TUG1 protects intestinal epithelial cells from damage induced by high glucose and high fat via AMPK/SIRT1.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Wei,Xingquan Wang,Yaqing Wei,Shilin Liu,Shengyu Gao,Hao Tian,Dewang Su

International journal of molecular sciences 23: PubMed35055078

2022

Effects of Endurance Training on the Coenzyme Q Redox State in Rat Heart, Liver, and Brain at the Tissue and Mitochondrial Levels: Implications for Reactive Oxygen Species Formation and Respiratory Chain Remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Karolina Dominiak,Lukasz Galganski,Adrianna Budzinska,Andrzej Woyda-Ploszczyca,Jerzy A Zoladz,Wieslawa Jarmuszkiewicz

Molecular medicine reports 25: PubMed34913071

2021

Phytochemical‑rich herbal formula ATG‑125 protects against sucrose‑induced gastrocnemius muscle atrophy by rescuing Akt signaling and improving mitochondrial dysfunction in young adult mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ching-Chuan Yeh,Hsuan-Miao Liu,Ming-Chung Lee,Yann-Lii Leu,Wei-Han Chiang,Hen-Hong Chang,Tzung-Yan Lee

Frontiers in cellular neuroscience 15:738533 PubMed34658794

2021

Neuroprotective Effects of Exogenous Irisin in Kainic Acid-Induced Status Epilepticus.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Cheng,Yaru Cui,Yujie Zhai,Wenyu Xin,Yan Yu,Jia Liang,Shucui Li,Hongliu Sun

International journal of molecular sciences 22: PubMed34360607

2021

Losartan Prevents Hepatic Steatosis and Macrophage Polarization by Inhibiting HIF-1α in a Murine Model of NAFLD.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng-Hui Wang,Hsuan-Miao Liu,Zi-Yu Chang,Tse-Hung Huang,Tzung-Yan Lee

Cancer chemotherapy and pharmacology 88:633-642 PubMed34146128

2021

UCP-2 inhibitor enhanced the efficacy of trastuzumab against HER2 positive breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Hua,Zhe Zhang,Lili Zhang,Yan Sun,Yuan Yuan

Annals of translational medicine 9:259 PubMed33708886

2021

The effects of UCP2 on autophagy through the AMPK signaling pathway in septic cardiomyopathy and the underlying mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Yu Mao,Long-Xiang Su,Dong-Kai Li,Hong-Min Zhang,Xiao-Ting Wang,Da-Wei Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-UCP2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP2 antibody (AB97931)

Western blot - Anti-UCP2 antibody (AB97931)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (33)

Product promise