Rabbit Polyclonal UCP3 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 21 publications. Immunogen corresponding to Synthetic Peptide within Human UCP3 aa 250-300 conjugated to Keyhole Limpet Haemocyanin.
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- International journal of molecular sciences 23:2022Molecular Liver Fingerprint Reflects the Seasonal Physiology of the Grey Mouse Lemur () during Winter.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBlandine Chazarin,Margaux Benhaim-Delarbre,Charlotte Brun,Aude Anzeraey,Fabrice Bertile,Jérémy TerrienPubMed 35457071
- International journal of molecular sciences 23:2022Gestational Exercise Increases Male Offspring's Maximal Workload Capacity Early in Life.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJorge Beleza,Jelena Stevanović-Silva,Pedro Coxito,Hugo Rocha,Paulo Santos,António Ascensão,Joan Ramon Torrella,José MagalhãesPubMed 35409278
- Molecular medicine reports 25:2021Phytochemical‑rich herbal formula ATG‑125 protects against sucrose‑induced gastrocnemius muscle atrophy by rescuing Akt signaling and improving mitochondrial dysfunction in young adult mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChing-Chuan Yeh,Hsuan-Miao Liu,Ming-Chung Lee,Yann-Lii Leu,Wei-Han Chiang,Hen-Hong Chang,Tzung-Yan LeePubMed 34913071
- Autophagy 18:1078-10892021Regulation and role of glycophagy in skeletal muscle energy metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTimothy D Heden,Lisa S Chow,Curtis C Hughey,Douglas G MashekPubMed 34506219
- Journal of applied physiology (Bethesda, Md. : 1985) 130:1317-13252021Effect of conditioning and physiological hyperthermia on canine skeletal muscle mitochondrial oxygen consumption.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael S Davis,Montana R BarrettPubMed 33661725
- Drug design, development and therapy 14:4251-42612020EGCG Upregulates UCP Levels to Protect MIN Pancreatic Islet Cells from Interleukin-1β-Induced Apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXu Jia,Ziren Luo,Ying Gao,Hua Liu,Xinghai Liu,Wenli Mai,Hong Liu,Qian ZhengPubMed 33116413
- Antioxidants (Basel, Switzerland) 9:2020EPA is Cardioprotective in Male Rats Subjected to Sepsis, but ALA Is Not Beneficial.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThibault Leger,Chrystèle Jouve,Isabelle Hininger-Favier,Jean-Paul Rigaudiere,Frédéric Capel,Vincent Sapin,Clarisse Moreau,Alice Charrier,Luc DemaisonPubMed 32365668
- Experimental & molecular medicine 52:485-4962020The essential role of fructose-1,6-bisphosphatase 2 enzyme in thermal homeostasis upon cold stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyun-Jun Park et. al.PubMed 32203098
- Cells 9:2020Effect of Dietary Silk Peptide on Obesity, Hyperglycemia, and Skeletal Muscle Regeneration in High-Fat Diet-Fed Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKippeum Lee et. al.PubMed 32041272
- Antioxidants (Basel, Switzerland) 8:2019Dietary EPA Increases Rat Mortality in Diabetes Mellitus, A Phenomenon Which Is Compensated by Green Tea Extract.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThibault Leger et. al.PubMed 31690052
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.097% Sodium azide
Constituents: 0.1% BSA, 0.0268% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human UCP3 aa 250-300 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P55916
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/3000 | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Putative transmembrane transporter that plays a role in mitochondrial metabolism via an as yet unclear mechanism (PubMed:21775425, PubMed:36114012). Originally, this mitochondrial protein was thought to act as a proton transmembrane transporter from the mitochondrial intermembrane space into the matrix, causing proton leaks through the inner mitochondrial membrane, thereby uncoupling mitochondrial membrane potential generation from ATP synthesis (PubMed:11171965, PubMed:12670931, PubMed:12734183, PubMed:9305858). However, this function is controversial and uncoupling may not be the function, or at least not the main function, but rather a consequence of more conventional metabolite transporter activity (PubMed:11707458).
Alternative names
SLC25A9, UCP3, Putative mitochondrial transporter UCP3, Solute carrier family 25 member 9, Uncoupling protein-3, UCP 3
Recommended products
Rabbit Polyclonal UCP3 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 21 publications. Immunogen corresponding to Synthetic Peptide within Human UCP3 aa 250-300 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.097% Sodium azide
Constituents: 0.1% BSA, 0.0268% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human UCP3 aa 250-300 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P55916
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
The antibody is affinity-purified using the immunogen peptide immobilized on agarose.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Storage in frost-free freezers is also not recommended.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Working dilution samples should be discarded if not used within 12 hours.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
UCP3 also known as uncoupling protein 3 is a member of the mitochondrial transporter superfamily. It has a molecular mass of approximately 33 kDa. This protein is predominantly found in skeletal muscle and brown adipose tissue where it resides in the inner membrane of mitochondria. UCP3 functions by facilitating the proton leak across the mitochondrial membrane decoupling oxidative phosphorylation from ATP synthesis and therefore reducing the mitochondrial membrane potential.
- Biological function summary
The function of UCP3 is involved in the regulation of energy metabolism. It modulates the production of heat by dissipating the proton gradient which otherwise drives ATP synthesis. This makes it an important actor in thermoregulation and metabolic efficiency. UCP3 forms part of a broader set of uncoupling proteins which include UCP1 and UCP2 each playing slightly different roles in energy metabolism and mitochondrial function.
- Pathways
UCP3 influences the energy balance within cells by integrating into metabolic processes like fatty acid oxidation and thermogenesis. It plays a role in the mitochondrial oxidative phosphorylation pathway. UCP3's activity in these pathways also links it to other proteins such as UCP1 which is important in brown fat for thermogenesis and it interacts indirectly with enzymes involved in fatty acid metabolism.
- Associated diseases and disorders
Researchers associate UCP3 with metabolic conditions such as obesity and type 2 diabetes. Altered UCP3 expression can impact the efficiency of energy expenditure and thermoregulation potentially leading to these disorders. In the context of obesity and diabetes UCP3's interaction with other proteins like insulin-related signaling molecules highlights its indirect role in glucose homeostasis and lipid metabolism dysfunction.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP3 antibody (ab10985)
ab10985 (2µg/ml) staining UCP3 in human skeletal muscle using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of myocytes.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
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