AB10985

Anti-UCP3 antibody

Name: Anti-UCP3 antibody (ab10985) | Abcam
Brand: Abcam Limited
SKU: AB10985
Rating: 5 (1 reviews)

(1 Review)

(23 Publications)

Rabbit Polyclonal UCP3 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 23 publications. Immunogen corresponding to Synthetic Peptide within Human UCP3 aa 250-300 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

SLC25A9, UCP3, Putative mitochondrial transporter UCP3, Solute carrier family 25 member 9, Uncoupling protein-3, UCP 3

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP3 antibody (AB10985)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP3 antibody (AB10985)

ab10985 (2µg/ml) staining UCP3 in human skeletal muscle using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of myocytes.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human UCP3 aa 250-300 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P55916

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/3000", "IHCP-species-notes": "<p></p>" } } }

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Product details

Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

The antibody is affinity-purified using the immunogen peptide immobilized on agarose.

Storage buffer

Preservative: 0.097% Sodium azide Constituents: PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UCP3 also known as uncoupling protein 3 is a member of the mitochondrial transporter superfamily. It has a molecular mass of approximately 33 kDa. This protein is predominantly found in skeletal muscle and brown adipose tissue where it resides in the inner membrane of mitochondria. UCP3 functions by facilitating the proton leak across the mitochondrial membrane decoupling oxidative phosphorylation from ATP synthesis and therefore reducing the mitochondrial membrane potential.

Biological function summary

The function of UCP3 is involved in the regulation of energy metabolism. It modulates the production of heat by dissipating the proton gradient which otherwise drives ATP synthesis. This makes it an important actor in thermoregulation and metabolic efficiency. UCP3 forms part of a broader set of uncoupling proteins which include UCP1 and UCP2 each playing slightly different roles in energy metabolism and mitochondrial function.

Pathways

UCP3 influences the energy balance within cells by integrating into metabolic processes like fatty acid oxidation and thermogenesis. It plays a role in the mitochondrial oxidative phosphorylation pathway. UCP3's activity in these pathways also links it to other proteins such as UCP1 which is important in brown fat for thermogenesis and it interacts indirectly with enzymes involved in fatty acid metabolism.

Researchers associate UCP3 with metabolic conditions such as obesity and type 2 diabetes. Altered UCP3 expression can impact the efficiency of energy expenditure and thermoregulation potentially leading to these disorders. In the context of obesity and diabetes UCP3's interaction with other proteins like insulin-related signaling molecules highlights its indirect role in glucose homeostasis and lipid metabolism dysfunction.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Putative transmembrane transporter that plays a role in mitochondrial metabolism via an as yet unclear mechanism (PubMed : 21775425, PubMed : 36114012). Originally, this mitochondrial protein was thought to act as a proton transmembrane transporter from the mitochondrial intermembrane space into the matrix, causing proton leaks through the inner mitochondrial membrane, thereby uncoupling mitochondrial membrane potential generation from ATP synthesis (PubMed : 11171965, PubMed : 12670931, PubMed : 12734183, PubMed : 9305858). However, this function is controversial and uncoupling may not be the function, or at least not the main function, but rather a consequence of more conventional metabolite transporter activity (PubMed : 11707458).

See full target information UCP3

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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed35457071

2022

Molecular Liver Fingerprint Reflects the Seasonal Physiology of the Grey Mouse Lemur () during Winter.

Applications

Unspecified application

Species

Unspecified reactive species

Blandine Chazarin,Margaux Benhaim-Delarbre,Charlotte Brun,Aude Anzeraey,Fabrice Bertile,Jérémy Terrien

International journal of molecular sciences 23: PubMed35409278

2022

Gestational Exercise Increases Male Offspring's Maximal Workload Capacity Early in Life.

Applications

Unspecified application

Species

Unspecified reactive species

Jorge Beleza,Jelena Stevanović-Silva,Pedro Coxito,Hugo Rocha,Paulo Santos,António Ascensão,Joan Ramon Torrella,José Magalhães

Molecular medicine reports 25: PubMed34913071

2021

Phytochemical‑rich herbal formula ATG‑125 protects against sucrose‑induced gastrocnemius muscle atrophy by rescuing Akt signaling and improving mitochondrial dysfunction in young adult mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ching-Chuan Yeh,Hsuan-Miao Liu,Ming-Chung Lee,Yann-Lii Leu,Wei-Han Chiang,Hen-Hong Chang,Tzung-Yan Lee

Autophagy 18:1078-1089 PubMed34506219

2021

Regulation and role of glycophagy in skeletal muscle energy metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Timothy D Heden,Lisa S Chow,Curtis C Hughey,Douglas G Mashek

Journal of applied physiology (Bethesda, Md. : 1985) 130:1317-1325 PubMed33661725

2021

Effect of conditioning and physiological hyperthermia on canine skeletal muscle mitochondrial oxygen consumption.

Applications

Unspecified application

Species

Unspecified reactive species

Michael S Davis,Montana R Barrett

Drug design, development and therapy 14:4251-4261 PubMed33116413

2020

EGCG Upregulates UCP Levels to Protect MIN Pancreatic Islet Cells from Interleukin-1β-Induced Apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Jia,Ziren Luo,Ying Gao,Hua Liu,Xinghai Liu,Wenli Mai,Hong Liu,Qian Zheng

Antioxidants (Basel, Switzerland) 9: PubMed32365668

2020

EPA is Cardioprotective in Male Rats Subjected to Sepsis, but ALA Is Not Beneficial.

Applications

Unspecified application

Species

Unspecified reactive species

Thibault Leger,Chrystèle Jouve,Isabelle Hininger-Favier,Jean-Paul Rigaudiere,Frédéric Capel,Vincent Sapin,Clarisse Moreau,Alice Charrier,Luc Demaison

Experimental & molecular medicine 52:485-496 PubMed32203098

2020

The essential role of fructose-1,6-bisphosphatase 2 enzyme in thermal homeostasis upon cold stress.

Applications

Unspecified application

Species

Unspecified reactive species

Hyun-Jun Park,Hye Rim Jang,Shi-Young Park,Young-Bum Kim,Hui-Young Lee,Cheol Soo Choi

Metabolism: clinical and experimental 106:154191 PubMed32112822

2020

Low-density lipoprotein receptor-related protein 1 deficiency in cardiomyocytes reduces susceptibility to insulin resistance and obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Aleyda Benitez-Amaro,Elena Revuelta-López,Olga Bornachea,Lídia Cedó,Àngela Vea,Laura Herrero,Nuria Roglans,Carolina Soler-Botija,David de Gonzalo-Calvo,Laura Nasarre,Sandra Camino-López,Eduardo García,Eugenia Mato,Francisco Blanco-Vaca,Antoni Bayes-Genis,David Sebastian,Joan Carles Laguna,Dolors Serra,Antonio Zorzano,Joan Carles Escola-Gil,Vicenta Llorente-Cortes

Cells 9: PubMed32041272

2020

Effect of Dietary Silk Peptide on Obesity, Hyperglycemia, and Skeletal Muscle Regeneration in High-Fat Diet-Fed Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kippeum Lee,Heegu Jin,Sungwoo Chei,Hyun-Ji Oh,Jeong-Yong Lee,Boo-Yong Lee

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-UCP3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP3 antibody (AB10985)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (23)

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