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AB180643

Anti-UCP3 antibody

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(7 Publications)

Rabbit Polyclonal UCP3 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human UCP3 aa 1-150.

View Alternative Names

SLC25A9, UCP3, Putative mitochondrial transporter UCP3, Solute carrier family 25 member 9, Uncoupling protein-3, UCP 3

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-UCP3 antibody (AB180643)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-UCP3 antibody (AB180643)

Immunocytochemistry/Immunofluorescence analysis of MCF7 cells using ab180643. Blue DAPI for nuclear staining.

Western blot - Anti-UCP3 antibody (AB180643)
  • WB

Supplier Data

Western blot - Anti-UCP3 antibody (AB180643)

All lanes:

Western blot - Anti-UCP3 antibody (ab180643) at 1/500 dilution

Lane 1:

mouse testis cell line extract

Lane 2:

mouse liver cell line extract

Lane 3:

mouse lung cell line extract

Predicted band size: 34 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human UCP3 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

P55916

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UCP3 also known as uncoupling protein 3 is a member of the mitochondrial transporter superfamily. It has a molecular mass of approximately 33 kDa. This protein is predominantly found in skeletal muscle and brown adipose tissue where it resides in the inner membrane of mitochondria. UCP3 functions by facilitating the proton leak across the mitochondrial membrane decoupling oxidative phosphorylation from ATP synthesis and therefore reducing the mitochondrial membrane potential.
Biological function summary

The function of UCP3 is involved in the regulation of energy metabolism. It modulates the production of heat by dissipating the proton gradient which otherwise drives ATP synthesis. This makes it an important actor in thermoregulation and metabolic efficiency. UCP3 forms part of a broader set of uncoupling proteins which include UCP1 and UCP2 each playing slightly different roles in energy metabolism and mitochondrial function.

Pathways

UCP3 influences the energy balance within cells by integrating into metabolic processes like fatty acid oxidation and thermogenesis. It plays a role in the mitochondrial oxidative phosphorylation pathway. UCP3's activity in these pathways also links it to other proteins such as UCP1 which is important in brown fat for thermogenesis and it interacts indirectly with enzymes involved in fatty acid metabolism.

Researchers associate UCP3 with metabolic conditions such as obesity and type 2 diabetes. Altered UCP3 expression can impact the efficiency of energy expenditure and thermoregulation potentially leading to these disorders. In the context of obesity and diabetes UCP3's interaction with other proteins like insulin-related signaling molecules highlights its indirect role in glucose homeostasis and lipid metabolism dysfunction.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Putative transmembrane transporter that plays a role in mitochondrial metabolism via an as yet unclear mechanism (PubMed : 21775425, PubMed : 36114012). Originally, this mitochondrial protein was thought to act as a proton transmembrane transporter from the mitochondrial intermembrane space into the matrix, causing proton leaks through the inner mitochondrial membrane, thereby uncoupling mitochondrial membrane potential generation from ATP synthesis (PubMed : 11171965, PubMed : 12670931, PubMed : 12734183, PubMed : 9305858). However, this function is controversial and uncoupling may not be the function, or at least not the main function, but rather a consequence of more conventional metabolite transporter activity (PubMed : 11707458).
See full target information UCP3
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Experimental physiology 109:562-575 PubMed38180279

2024

Postnatal growth restriction alters myocardial mitochondrial energetics in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph R Visker,Eric C Leszczynski,Austin G Wellette-Hunsucker,Ashley C McPeek,Melissa A Quinn,Seong Hyun Kim,Jason N Bazil,David P Ferguson

The Journal of nutritional biochemistry 124:109535 PubMed37984734

2023

Raspberry polyphenols target molecular pathways of heart failure.

Applications

Unspecified application

Species

Unspecified reactive species

Rami S Najjar,Ranjan K Roy,Javier E Stern,Rafaela G Feresin

Antioxidants (Basel, Switzerland) 12: PubMed36829970

2023

Inhibition of Mitochondrial Uncoupling Proteins Arrests Human Spermatozoa Motility without Compromising Viability.

Applications

Unspecified application

Species

Unspecified reactive species

David F Carrageta,Laís Freire-Brito,Bárbara Guerra-Carvalho,João C Ribeiro,Bruno S Monteiro,Alberto Barros,Pedro F Oliveira,Mariana P Monteiro,Marco G Alves

Nutrients 14: PubMed35807797

2022

Positive Effects of a Mediterranean Diet Supplemented with Almonds on Female Adipose Tissue Biology in Severe Obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Óscar Osorio-Conles,Romina Olbeyra,Violeta Moizé,Ainitze Ibarzabal,Oriol Giró,Judith Viaplana,Amanda Jiménez,Josep Vidal,Ana de Hollanda

Diabetes 70:2518-2531 PubMed34526367

2021

Activation of HIF1α Rescues the Hypoxic Response and Reverses Metabolic Dysfunction in the Diabetic Heart.

Applications

Unspecified application

Species

Unspecified reactive species

Maria da Luz Sousa Fialho,Ujang Purnama,Kaitlyn M J H Dennis,Claudia N Montes Aparicio,Marcos Castro-Guarda,Emmanuelle Massourides,Damian J Tyler,Carolyn A Carr,Lisa C Heather

Oxidative medicine and cellular longevity 2021:5566053 PubMed34326919

2021

Protective Effect of Jiang Tang Xiao Ke Granules against Skeletal Muscle IR via Activation of the AMPK/SIRT1/PGC-1 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Bai,Jiacheng Zuo,Xin Fang,Rufeng Ma,Tian Tian,Fangfang Mo,Qianqian Mu,Yi Zhang,Na Yu,Xueli Bao,Dongwei Zhang,Sihua Gao,Dandan Zhao

Frontiers in physiology 12:678985 PubMed34220544

2021

Mitochondrial Adaptation to Diet and Swimming Activity in Gilthead Seabream: Improved Nutritional Efficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Miquel Perelló-Amorós,Jaume Fernández-Borràs,Albert Sánchez-Moya,Emilio J Vélez,Isabel García-Pérez,Joaquin Gutiérrez,Josefina Blasco
View all publications
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