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AB61299

Anti-UCP3 antibody [mAbcam61299]

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(2 Publications)

Mouse Monoclonal UCP3 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

SLC25A9, UCP3, Putative mitochondrial transporter UCP3, Solute carrier family 25 member 9, Uncoupling protein-3, UCP 3

1 Images
Western blot - Anti-UCP3 antibody [mAbcam61299] (AB61299)
  • WB

Unknown

Western blot - Anti-UCP3 antibody [mAbcam61299] (AB61299)

All lanes:

Western blot - Anti-UCP3 antibody [mAbcam61299] (ab61299) at 10 µg/mL

All lanes:

Human skeletal muscle tissue lysate - total protein (<a href='/en-us/products/unavailable/human-skeletal-muscle-tissue-lysate-total-protein-ab29330'>ab29330</a>) at 10 µg

Secondary

All lanes:

Goat polyclonal Secondary Antibody to Mouse IgM - H&L (HRP), pre-adsorbed at 1/5000 dilution

Predicted band size: 34 kDa

Observed band size: 17 kDa,30 kDa

true

Exposure time: 2min

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

mAbcam61299

Isotype

IgM

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "10 µg/mL", "WB-species-notes": "<p></p>" }, "Macaque monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
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Properties and storage information

Purification notes
Tissue culture supernatant was cross-flow concentrated and buffer exchanged to PBS.
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UCP3 also known as uncoupling protein 3 is a member of the mitochondrial transporter superfamily. It has a molecular mass of approximately 33 kDa. This protein is predominantly found in skeletal muscle and brown adipose tissue where it resides in the inner membrane of mitochondria. UCP3 functions by facilitating the proton leak across the mitochondrial membrane decoupling oxidative phosphorylation from ATP synthesis and therefore reducing the mitochondrial membrane potential.
Biological function summary

The function of UCP3 is involved in the regulation of energy metabolism. It modulates the production of heat by dissipating the proton gradient which otherwise drives ATP synthesis. This makes it an important actor in thermoregulation and metabolic efficiency. UCP3 forms part of a broader set of uncoupling proteins which include UCP1 and UCP2 each playing slightly different roles in energy metabolism and mitochondrial function.

Pathways

UCP3 influences the energy balance within cells by integrating into metabolic processes like fatty acid oxidation and thermogenesis. It plays a role in the mitochondrial oxidative phosphorylation pathway. UCP3's activity in these pathways also links it to other proteins such as UCP1 which is important in brown fat for thermogenesis and it interacts indirectly with enzymes involved in fatty acid metabolism.

Researchers associate UCP3 with metabolic conditions such as obesity and type 2 diabetes. Altered UCP3 expression can impact the efficiency of energy expenditure and thermoregulation potentially leading to these disorders. In the context of obesity and diabetes UCP3's interaction with other proteins like insulin-related signaling molecules highlights its indirect role in glucose homeostasis and lipid metabolism dysfunction.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Putative transmembrane transporter that plays a role in mitochondrial metabolism via an as yet unclear mechanism (PubMed : 21775425, PubMed : 36114012). Originally, this mitochondrial protein was thought to act as a proton transmembrane transporter from the mitochondrial intermembrane space into the matrix, causing proton leaks through the inner mitochondrial membrane, thereby uncoupling mitochondrial membrane potential generation from ATP synthesis (PubMed : 11171965, PubMed : 12670931, PubMed : 12734183, PubMed : 9305858). However, this function is controversial and uncoupling may not be the function, or at least not the main function, but rather a consequence of more conventional metabolite transporter activity (PubMed : 11707458).
See full target information UCP3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cells 13: PubMed38667324

2024

The Role of -Adrenergic Receptors in Cold-Induced Beige Adipocyte Production in Pigs.

Applications

Unspecified application

Species

Unspecified reactive species

Shuo Yang,Hong Ma,Liang Wang,Fang Wang,Jiqiao Xia,Dongyu Liu,Linlin Mu,Xiuqin Yang,Di Liu

Frontiers in medicine 9:812653 PubMed35770009

2022

Solamargine Alleviated UVB-Induced Inflammation and Melanogenesis in Human Keratinocytes and Melanocytes the p38 MAPK Signaling Pathway, a Promising Agent for Post-inflammatory Hyperpigmentation.

Applications

Unspecified application

Species

Unspecified reactive species

Juemin Zhao,Yanjun Dan,Ziqi Liu,Qianqian Wang,Min Jiang,Chengfeng Zhang,Hamm-Ming Sheu,Chrang-Shi Lin,Leihong Xiang
View all publications
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