AB193470

Anti-UCP3 + UCP1 antibody [EPR19006]

RabMAb
Recombinant
20ul selling size
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(3 Publications)

Rabbit Recombinant Monoclonal UCP1 antibody. Suitable for IP, WB and reacts with Mouse, Rat, Human, Recombinant full length protein - Mouse samples. Cited in 3 publications.

View Alternative Names

SLC25A7, UCP, UCP1, Mitochondrial brown fat uncoupling protein 1, UCP 1, Solute carrier family 25 member 7, Thermogenin

5 Images

Supplier Data

Immunoprecipitation - Anti-UCP3 + UCP1 antibody [EPR19006] (AB193470)

UCP3 + UCP1 were immunoprecipitated from 1mg of Mouse skeletal muscle whole cell lysate with ab193470 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab193470 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : Mouse skeletal muscle whole cell lysate, 10μg (Input).

Lane 2 : ab193470 IP in Mouse skeletal muscle whole cell lysate.

Lane 3 : Rabbit IgG, monoclonal - Isotype Control (ab172730) instead of ab193470 in Mouse skeletal muscle whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

All lanes:

Immunoprecipitation - Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470)

false

Supplier Data

Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (AB193470)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 1 minute; Lane 2 : 15 seconds.

The expression profile observed is consistent with what has been described in the literature (PMID : 9080264).

All lanes:

Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470) at 1/1000 dilution

Lane 1:

Mouse brown adipose lysate at 10 µg

Lane 2:

Rat brown adipose lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 33 kDa,34 kDa

Observed band size: 34 kDa

false

Supplier Data

Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (AB193470)

Blocking/Dilution buffer : 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID : 9080264)

All lanes:

Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470) at 1/1000 dilution

Lane 1:

Mouse muscle tissue lysate at 20 µg

Lane 2:

Mouse lung tissue lysate at 20 µg

Lane 3:

C2C12 (Mouse myoblast cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 33 kDa,34 kDa,83 kDa

Observed band size: 28 kDa,34 kDa,83 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (AB193470)

Blocking/Dilution buffer : 5% NFDM/TBST.

Mouse UCP1 full length recombinant protein contains aa1-307 with a His-Tag®. Mouse UCP2 full length recombinant protein contains aa1-309 with a His-Tag®. These two full length recombinant proteins were made in-house

All lanes:

Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470) at 1/1000 dilution

Lane 1:

Mouse UCP1 full length recombinant protein at 0.02 µg

Lane 2:

Mouse UCP2 full length recombinant protein at 0.02 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 33 kDa,34 kDa

Observed band size: 34 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (AB193470)

Blocking/Dilution buffer : 5% NFDM/TBST.

Mouse UCP3 full length recombinant protein contains aa1-308 with a GST-Tag. This full length recombinant protein was made in-house.

All lanes:

Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470) at 1/1000 dilution

All lanes:

Mouse UCP3 full length recombinant protein at 0.02 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 33 kDa,34 kDa

Observed band size: 34 kDa

false

Exposure time: 1s

Carrier free

Anti-UCP3+UCP1 antibody [EPR19006] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19006

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Recombinant full length protein - Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UCP3 also known as Uncoupling protein 3 is a mitochondrial protein that plays a role in the process of thermogenesis by dissipating the proton gradient. It is a member of the mitochondrial anion carrier proteins (MACP) and has a molecular mass of approximately 33 kDa. UCP3 is mostly expressed in skeletal muscle and to a lesser extent in brown adipose tissue. Its homolog UCP1 sometimes referred to as thermogenin has a similar function in brown adipose tissue where it participates prominently in heat production.

Biological function summary

Uncoupling proteins like UCP3 facilitate the regulation of energy balance and metabolism. They act by uncoupling ATP synthesis from the electrochemical gradient therefore reducing reactive oxygen species production. UCP3 is not part of a protein complex but interacts closely with the mitochondrial environment. UCP1 on the other hand is active in non-shivering thermogenesis within brown fat cells enabling organisms to fend off hypothermia.

Pathways

These uncoupling proteins play roles in energy homeostasis and lipid metabolism. UCP3 participates significantly in the fatty acid oxidation pathway influencing how cells utilize fatty acids for energy. UCP1 is an important player in the mitochondrial oxidative phosphorylation pathway where it influences heat production and energy expenditure. They are both connected to the broader metabolic pathways essential for maintaining energy balance interacting with other mitochondrial carriers.

UCP3 and UCP1 are associated with conditions like obesity and type 2 diabetes. UCP3 helps protect against lipid-induced mitochondrial damage which can be a factor in insulin resistance. Meanwhile UCP1’s disruption could lead to decreased ability in non-shivering thermogenesis contributing to weight gain. Both proteins hold therapeutic promise as targets to modulate metabolic conditions by influencing the body's energy expenditure.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mitochondrial protein responsible for thermogenic respiration, a specialized capacity of brown adipose tissue and beige fat that participates in non-shivering adaptive thermogenesis to temperature and diet variations and more generally to the regulation of energy balance (By similarity). Functions as a long-chain fatty acid/LCFA and proton symporter, simultaneously transporting one LCFA and one proton through the inner mitochondrial membrane (PubMed : 24196960, PubMed : 28781081). However, LCFAs remaining associated with the transporter via their hydrophobic tails, it results in an apparent transport of protons activated by LCFAs. Thereby, dissipates the mitochondrial proton gradient and converts the energy of substrate oxydation into heat instead of ATP. Regulates the production of reactive oxygen species/ROS by mitochondria (By similarity).

See full target information UCP1

Additional targets

UCP3

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

PLoS genetics 21:e1011827 PubMed40825031

2025

Dynamics of recombination, X inactivation and centromere proteins during stick insect spermatogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Zoé Dumas,William Toubiana,Marie Delattre,Tanja Schwander

Cell death and differentiation 29:709-721 PubMed34621049

2021

Negative feedback of SNRK to circ-SNRK regulates cardiac function post-myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Yan Wang,Xiao-Xiao Liu,Yun-Fei Deng

Journal of the American Heart Association 10:e022556 PubMed34533037

2021

UCP3 (Uncoupling Protein 3) Insufficiency Exacerbates Left Ventricular Diastolic Dysfunction During Angiotensin II-Induced Hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Chen,Sadia Ashraf,Nadia Ashraf,Romain Harmancey

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-UCP3 + UCP1 antibody [EPR19006]

Immunoprecipitation - Anti-UCP3 + UCP1 antibody [EPR19006] (AB193470)

Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (AB193470)

Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (AB193470)

Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (AB193470)

Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (AB193470)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (3)

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