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Rabbit Recombinant Monoclonal UCP1 antibody. Suitable for IP, WB and reacts with Mouse, Rat, Human, Recombinant full length protein - Mouse samples. Cited in 2 publications.

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Images

Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (AB193470), expandable thumbnail
  • Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (AB193470), expandable thumbnail
  • Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (AB193470), expandable thumbnail
  • Immunoprecipitation - Anti-UCP3 + UCP1 antibody [EPR19006] (AB193470), expandable thumbnail
  • Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (AB193470), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Expected
Tested
Mouse
Tested
Tested
Rat
Expected
Tested
Recombinant full length protein - Mouse
Not recommended
Tested

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

-

Expected
Expected

Species
Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant full length protein - Mouse
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Target data

Function

Mitochondrial protein responsible for thermogenic respiration, a specialized capacity of brown adipose tissue and beige fat that participates in non-shivering adaptive thermogenesis to temperature and diet variations and more generally to the regulation of energy balance (By similarity). Functions as a long-chain fatty acid/LCFA and proton symporter, simultaneously transporting one LCFA and one proton through the inner mitochondrial membrane (PubMed:24196960, PubMed:28781081). However, LCFAs remaining associated with the transporter via their hydrophobic tails, it results in an apparent transport of protons activated by LCFAs. Thereby, dissipates the mitochondrial proton gradient and converts the energy of substrate oxydation into heat instead of ATP. Regulates the production of reactive oxygen species/ROS by mitochondria (By similarity).

Additional Targets

UCP3

Alternative names

SLC25A7, UCP, UCP1, Mitochondrial brown fat uncoupling protein 1, UCP 1, Solute carrier family 25 member 7, Thermogenin

Recommended products

Rabbit Recombinant Monoclonal UCP1 antibody. Suitable for IP, WB and reacts with Mouse, Rat, Human, Recombinant full length protein - Mouse samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR19006
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

UCP3 also known as Uncoupling protein 3 is a mitochondrial protein that plays a role in the process of thermogenesis by dissipating the proton gradient. It is a member of the mitochondrial anion carrier proteins (MACP) and has a molecular mass of approximately 33 kDa. UCP3 is mostly expressed in skeletal muscle and to a lesser extent in brown adipose tissue. Its homolog UCP1 sometimes referred to as thermogenin has a similar function in brown adipose tissue where it participates prominently in heat production.

Biological function summary

Uncoupling proteins like UCP3 facilitate the regulation of energy balance and metabolism. They act by uncoupling ATP synthesis from the electrochemical gradient therefore reducing reactive oxygen species production. UCP3 is not part of a protein complex but interacts closely with the mitochondrial environment. UCP1 on the other hand is active in non-shivering thermogenesis within brown fat cells enabling organisms to fend off hypothermia.

Pathways

These uncoupling proteins play roles in energy homeostasis and lipid metabolism. UCP3 participates significantly in the fatty acid oxidation pathway influencing how cells utilize fatty acids for energy. UCP1 is an important player in the mitochondrial oxidative phosphorylation pathway where it influences heat production and energy expenditure. They are both connected to the broader metabolic pathways essential for maintaining energy balance interacting with other mitochondrial carriers.

Associated diseases and disorders

UCP3 and UCP1 are associated with conditions like obesity and type 2 diabetes. UCP3 helps protect against lipid-induced mitochondrial damage which can be a factor in insulin resistance. Meanwhile UCP1’s disruption could lead to decreased ability in non-shivering thermogenesis contributing to weight gain. Both proteins hold therapeutic promise as targets to modulate metabolic conditions by influencing the body's energy expenditure.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470), expandable thumbnail

    Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Mouse UCP1 full length recombinant protein contains aa1-307 with a His-Tag®. Mouse UCP2 full length recombinant protein contains aa1-309 with a His-Tag®. These two full length recombinant proteins were made in-house

    All lanes: Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470) at 1/1000 dilution

    Lane 1: Mouse UCP1 full length recombinant protein at 0.02 µg

    Lane 2: Mouse UCP2 full length recombinant protein at 0.02 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 33 kDa, 34 kDa

    Observed band size: 34 kDa

    Exposure time: 3min

  • Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470), expandable thumbnail

    Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Mouse UCP3 full length recombinant protein contains aa1-308 with a GST-Tag. This full length recombinant protein was made in-house.

    All lanes: Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470) at 1/1000 dilution

    All lanes: Mouse UCP3 full length recombinant protein at 0.02 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 33 kDa, 34 kDa

    Observed band size: 34 kDa

    Exposure time: 1s

  • Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470), expandable thumbnail

    Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID: 9080264)

    All lanes: Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470) at 1/1000 dilution

    Lane 1: Mouse muscle tissue lysate at 20 µg

    Lane 2: Mouse lung tissue lysate at 20 µg

    Lane 3: C2C12 (Mouse myoblast cell line) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 33 kDa, 34 kDa, 83 kDa

    Observed band size: 28 kDa, 34 kDa, 83 kDa

    Exposure time: 3min

  • Immunoprecipitation - Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470), expandable thumbnail

    Immunoprecipitation - Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470)

    UCP3 + UCP1 were immunoprecipitated from 1mg of Mouse skeletal muscle whole cell lysate with ab193470 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab193470 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Mouse skeletal muscle whole cell lysate, 10μg (Input).

    Lane 2: ab193470 IP in Mouse skeletal muscle whole cell lysate.

    Lane 3: Rabbit IgG, monoclonal - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab193470 in Mouse skeletal muscle whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    All lanes: Immunoprecipitation - Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470)

  • Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470), expandable thumbnail

    Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 1 minute; Lane 2: 15 seconds.

    The expression profile observed is consistent with what has been described in the literature (PMID: 9080264).

    All lanes: Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470) at 1/1000 dilution

    Lane 1: Mouse brown adipose lysate at 10 µg

    Lane 2: Rat brown adipose lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 33 kDa, 34 kDa

    Observed band size: 34 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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