JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB251173

Anti-UCP3+UCP1 antibody [EPR19006] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal UCP1 antibody. Carrier free. Suitable for IP, WB and reacts with Mouse, Human, Rat samples.

View Alternative Names

SLC25A7, UCP, UCP1, Mitochondrial brown fat uncoupling protein 1, UCP 1, Solute carrier family 25 member 7, Thermogenin, SLC25A9, UCP3, Putative mitochondrial transporter UCP3, Solute carrier family 25 member 9, Uncoupling protein-3, UCP 3

5 Images
Immunoprecipitation - Anti-UCP3+UCP1 antibody [EPR19006] - BSA and Azide free (AB251173)
  • IP

Supplier Data

Immunoprecipitation - Anti-UCP3+UCP1 antibody [EPR19006] - BSA and Azide free (AB251173)

This data was developed using ab193470, the same antibody clone in a different buffer formulation.

UCP3 + UCP1 were immunoprecipitated from 1mg of Mouse skeletal muscle whole cell lysate with ab193470 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab193470 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : Mouse skeletal muscle whole cell lysate, 10μg (Input).
Lane 2 : ab193470 IP in Mouse skeletal muscle whole cell lysate.
Lane 3 : Rabbit IgG, monoclonal - Isotype Control (ab172730) instead of ab193470 in Mouse skeletal muscle whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

All lanes:

Immunoprecipitation - Anti-UCP3 + UCP1 antibody [EPR19006] (<a href='/en-us/products/primary-antibodies/ucp3-ucp1-antibody-epr19006-ab193470'>ab193470</a>)

false

Western blot - Anti-UCP3+UCP1 antibody [EPR19006] - BSA and Azide free (AB251173)
  • WB

Supplier Data

Western blot - Anti-UCP3+UCP1 antibody [EPR19006] - BSA and Azide free (AB251173)

This data was developed using ab193470, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 1 minute; Lane 2 : 15 seconds.

The expression profile observed is consistent with what has been described in the literature (PMID : 9080264).

All lanes:

Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (<a href='/en-us/products/primary-antibodies/ucp3-ucp1-antibody-epr19006-ab193470'>ab193470</a>) at 1/1000 dilution

Lane 1:

Mouse brown adipose lysate at 10 µg

Lane 2:

Rat brown adipose lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 33 kDa,34 kDa

Observed band size: 34 kDa

false

Western blot - Anti-UCP3+UCP1 antibody [EPR19006] - BSA and Azide free (AB251173)
  • WB

Supplier Data

Western blot - Anti-UCP3+UCP1 antibody [EPR19006] - BSA and Azide free (AB251173)

This data was developed using ab193470, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID : 9080264).

All lanes:

Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (<a href='/en-us/products/primary-antibodies/ucp3-ucp1-antibody-epr19006-ab193470'>ab193470</a>) at 1/1000 dilution

Lane 1:

Mouse muscle tissue lysate at 20 µg

Lane 2:

Mouse lung tissue lysate at 20 µg

Lane 3:

C2C12 (Mouse myoblast cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 33 kDa,34 kDa,83 kDa

Observed band size: 28 kDa,34 kDa,83 kDa

false

Exposure time: 3min

Western blot - Anti-UCP3+UCP1 antibody [EPR19006] - BSA and Azide free (AB251173)
  • WB

Supplier Data

Western blot - Anti-UCP3+UCP1 antibody [EPR19006] - BSA and Azide free (AB251173)

This data was developed using ab193470, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Mouse UCP1 full length recombinant protein contains aa1-307 with a His-Tag®. Mouse UCP2 full length recombinant protein contains aa1-309 with a His-Tag®. These two full length recombinant proteins were made in-house.

All lanes:

Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (<a href='/en-us/products/primary-antibodies/ucp3-ucp1-antibody-epr19006-ab193470'>ab193470</a>) at 1/1000 dilution

Lane 1:

Mouse UCP1 full length recombinant protein at 0.02 µg

Lane 2:

Mouse UCP2 full length recombinant protein at 0.02 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 33 kDa,34 kDa

Observed band size: 34 kDa

false

Exposure time: 3min

Western blot - Anti-UCP3+UCP1 antibody [EPR19006] - BSA and Azide free (AB251173)
  • WB

Supplier Data

Western blot - Anti-UCP3+UCP1 antibody [EPR19006] - BSA and Azide free (AB251173)

This data was developed using ab193470, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Mouse UCP3 full length recombinant protein contains aa1-308 with a GST-Tag. This full length recombinant protein was made in-house.

All lanes:

Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (<a href='/en-us/products/primary-antibodies/ucp3-ucp1-antibody-epr19006-ab193470'>ab193470</a>) at 1/1000 dilution

All lanes:

Mouse UCP3 full length recombinant protein at 0.02 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 33 kDa,34 kDa

Observed band size: 34 kDa

false

Exposure time: 1s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19006

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Assay Kits

AB65341

Free Fatty Acid Assay Kit - Quantification

Biochemical assay - Free Fatty Acid Assay Kit - Quantification (AB65341)

  • 5
  • 1
Primary Antibodies

AB72230

Anti-PGC1 alpha + beta antibody

Immunocytochemistry/ Immunofluorescence - Anti-PGC1 alpha + beta antibody (AB72230)

  • 4
  • 2
Primary Antibodies

AB67241

Anti-UCP2 antibody

Western blot - Anti-UCP2 antibody (AB67241)

  • 3
  • 3
Primary Antibodies

AB209483

Anti-UCP1 antibody [EPR20381]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP1 antibody [EPR20381] (AB209483)

  • 5
  • 4
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

ab251173 is the carrier-free version of ab193470.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Uncoupling proteins UCP3 and UCP1 are members of the mitochondrial anion carrier protein family. These proteins play significant roles in mitochondrial proton leak impacting the production of ATP and regulation of energy balance. UCP1 also known as thermogenin has a molecular mass of approximately 32 kDa and is primarily expressed in brown adipose tissue. In contrast UCP3 is found mostly in skeletal muscle and is slightly larger around 33 kDa. Both proteins facilitate the uncoupling process in mitochondria dissipating proton gradient energy as heat instead of ATP synthesis.
Biological function summary

UCP3 and UCP1 are involved in thermoregulation and energy metabolism. These proteins do not operate as part of a larger protein complex but contribute to distinct cellular processes. UCP1 generates heat in brown adipose tissue enabling thermogenesis a process important for maintaining body temperature in cold environments. UCP3 plays a role in regulating fatty acid metabolism and mitigating the effects of reactive oxygen species within muscle cells. This protein may protect mitochondria from damage due to oxidative stress.

Pathways

The roles UCP3 and UCP1 play intersect significantly with the oxidative phosphorylation and lipid metabolism pathways. UCP1 links closely to the thermogenesis pathway where it aids in converting stored energy in brown fat into heat alongside signaling proteins like PGC-1α. UCP3 integrates into pathways that involve the oxidation of fatty acids working with proteins such as peroxisome proliferator-activated receptors (PPARs) to regulate energy combustion in muscle tissues.

UCP3 and UCP1 have connections to obesity and type 2 diabetes. Low UCP1 activity can result in reduced thermogenesis and increased susceptibility to obesity with leptin and insulin involved in this dysfunctional process. UCP3 variations have associations with impaired fatty acid oxidation contributing to lipid accumulation in muscles and insulin resistance a prominent issue in type 2 diabetes. Understanding the modulation of these proteins offers avenues for therapeutic intervention in metabolic disorders.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Mitochondrial protein responsible for thermogenic respiration, a specialized capacity of brown adipose tissue and beige fat that participates in non-shivering adaptive thermogenesis to temperature and diet variations and more generally to the regulation of energy balance (By similarity). Functions as a long-chain fatty acid/LCFA and proton symporter, simultaneously transporting one LCFA and one proton through the inner mitochondrial membrane (PubMed : 24196960, PubMed : 28781081). However, LCFAs remaining associated with the transporter via their hydrophobic tails, it results in an apparent transport of protons activated by LCFAs. Thereby, dissipates the mitochondrial proton gradient and converts the energy of substrate oxydation into heat instead of ATP. Regulates the production of reactive oxygen species/ROS by mitochondria (By similarity).
See full target information UCP1

Additional targets

UCP3
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com