Anti-UFC1 antibody [EPR15014-102] - BSA and Azide free

• WB

Lab

Western blot - Anti-UFC1 antibody [EPR15014-102] - BSA and Azide free (AB250984)

This data was developed using the same antibody clone in a different buffer formulation (ab189252).

Lanes 1 - 4 : Merged signal (red and green). Green - ab189252 observed at 20 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab189252 was shown to react with UFC1 in wild-type HAP1 cells in western blot. Loss of signal was observed when UFC1 knockout sample was used. Wild-type HAP1 and UFC1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab189252 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-UFC1 antibody [EPR15014-102] (<a href='/en-us/products/primary-antibodies/ufc1-antibody-epr15014-102-ab189252'>ab189252</a>) at 1/10000 dilution

Lane 1:

Wild-type HAP1 cell lysate at 20 µg

Lane 2:

UFC1 knockout HAP1 cell lysate at 20 µg

Lane 3:

MCF7 cell lysate at 20 µg

Lane 4:

A549 cell lysate at 20 µg

Predicted band size: 19 kDa

Observed band size: 20 kDa

false

• WB

Lab

Western blot - Anti-UFC1 antibody [EPR15014-102] - BSA and Azide free (AB250984)

This data was developed using the same antibody clone in a different buffer formulation (ab189252).

Lanes 1- 2 : Merged signal (red and green). Green - ab189252 observed at 20 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab189252 was shown to react with UFC1 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266814 (knockout cell lysate ab257781) was used. Wild-type HEK-293T and UFC1 HEK-293T KO cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab189252 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-UFC1 antibody [EPR15014-102] (<a href='/en-us/products/primary-antibodies/ufc1-antibody-epr15014-102-ab189252'>ab189252</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

UFC1 knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human UFC1 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-ufc1-knockout-hek-293t-cell-line-ab266814'>ab266814</a>)

Predicted band size: 19 kDa

Observed band size: 20 kDa

false