Anti-UFD1L antibody [EPR12847] - N-terminal

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-UFD1L antibody [EPR12847] - N-terminal (AB181080)

Immunofluorescent analysis of 4% paraformaldehyde fixed A431 cells labeling UFD1L with ab181080 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG (Dylight 488) at 1/200 dilution. Conterstained with Dapi (blue).

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-UFD1L antibody [EPR12847] - N-terminal (AB181080)

Intracellular flow cytometric analysis of 293 cells (2%paraformaldehyde-fixed) labeling UFD1L with ab181080 at 1/30 dilution (red) or a rabbit IgG (negative) (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution. Isotype control : Rabbit IgG

• WB

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Western blot - Anti-UFD1L antibody [EPR12847] - N-terminal (AB181080)

All lanes:

Western blot - Anti-UFD1L antibody [EPR12847] - N-terminal (ab181080) at 1/2000 dilution

Lane 1:

Raji cell lysate at 1/1000 dilution

Lane 2:

HeLa cell lysate at 1/1000 dilution

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 35 kDa

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• WB

CiteAb

Western blot - Anti-UFD1L antibody [EPR12847] - N-terminal (AB181080)

UFD1L western blot using anti-UFD1L antibody [EPR12847] - N-terminal ab181080. Publication image and figure legend from Wang, T., Wang, B., et al., 2017, PLoS Pathog, PubMed 28985237.

ab181080 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab181080 please see the product overview.

EV71 targets ERAD at multiple points.(A) Diagram of the key molecules involved in ERAD. (B) RD cells were infected with EV71 (MOI = 10) for the indicated times (hpi : hours post-infection). The cells were then harvested and western blot was performed using the indicated antibodies to detect the indicated ERAD components, EV71 2C, and actin. The ERAD molecules assessed in this study were separated into four categories : substrate recognition, retrotranslocation, ubiquitination, and proteasomal degradation. Asterisks indicate the molecules that were obviously downregulated. (C) Full-size western blots for Herp, Hrd1, VIMP, and UBXD8 described in (B). (D) Quantification of Ubc6e, Herp, Hrd1, VIMP, and UBXD8 in (B). The data are presented as means ± SD of three independent experiments.

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