Rabbit Polyclonal UFL1 antibody. C-terminal. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human UFL1 aa 700 to C-terminus.
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- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e232212023UFMylation of HRD1 regulates endoplasmic reticulum homeostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Luo,Qi-Bin Jiao,Chuan-Bin Shen,Wen-Yan Gong,Jing-Hua Yuan,Ying-Ying Liu,Zhen Chen,Jiang Liu,Xiao-Ling Xu,Yu-Sheng Cong,Xing-Wei ZhangPubMed 37795761
- Reproductive biology and endocrinology : RB&E 20:842022Ubiquitin-like modifier 1 ligating enzyme 1 relieves cisplatin-induced premature ovarian failure by reducing endoplasmic reticulum stress in granulosa cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiangting Tang,Hao Dong,Zhi Fang,Jingyi Li,Qi Yang,Ting Yao,Zezheng PanPubMed 35610622
- Journal of cell science 133:2020ACSL3 is a novel GABARAPL2 interactor that links ufmylation and lipid droplet biogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFranziska Eck et. al.PubMed 32843575
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human UFL1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O94874
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Chimpanzee | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Cynomolgus monkey | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Horse | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Rabbit, Horse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species Mouse | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Rabbit, Horse | Dilution info - | Notes - |
Associated Products
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Target data
Function
E3 protein ligase that mediates ufmylation, the covalent attachment of the ubiquitin-like modifier UFM1 to lysine residues on target proteins, and which plays a key role in various processes, such as ribosome recycling, response to DNA damage, interferon response or reticulophagy (also called ER-phagy) (PubMed:20018847, PubMed:20164180, PubMed:20228063, PubMed:25219498, PubMed:27351204, PubMed:30626644, PubMed:30783677, PubMed:32160526, PubMed:32807901, PubMed:35394863, PubMed:36121123, PubMed:36543799, PubMed:36893266, PubMed:37036982, PubMed:37311461, PubMed:37595036, PubMed:37795761, PubMed:38377992, PubMed:38383785, PubMed:38383789). Catalyzes ufmylation of many protein, such as CD274/PD-L1, CDK5RAP3, CYB5R3, DDRGK1, EIF6, histone H4, MRE11, P4HB, PDCD1/PD-1, TRIP4, RPN1, RPS20/uS10, RPL10/uL16, RPL26/uL24, SYVN1/HRD1 and TP53/p53 (PubMed:20018847, PubMed:20531390, PubMed:25219498, PubMed:30783677, PubMed:30886146, PubMed:32160526, PubMed:35753586, PubMed:36543799, PubMed:36893266, PubMed:37036982, PubMed:37595036, PubMed:37795761, PubMed:38383785, PubMed:38383789). As part of the UREL complex, plays a key role in ribosome recycling by catalyzing mono-ufmylation of RPL26/uL24 subunit of the 60S ribosome (PubMed:38383785, PubMed:38383789). Ufmylation of RPL26/uL24 occurs on free 60S ribosomes following ribosome dissociation: it weakens the junction between post-termination 60S subunits and SEC61 translocons, promoting release and recycling of the large ribosomal subunit from the endoplasmic reticulum membrane (PubMed:38383785, PubMed:38383789). Ufmylation of RPL26/uL24 and subsequent 60S ribosome recycling either take place after normal termination of translation or after ribosome stalling during cotranslational translocation at the endoplasmic reticulum (PubMed:37036982, PubMed:37595036, PubMed:38383785, PubMed:38383789). Involved in reticulophagy in response to endoplasmic reticulum stress by mediating ufmylation of proteins such as CYB5R3 and RPN1, thereby promoting lysosomal degradation of ufmylated proteins (PubMed:23152784, PubMed:32160526, PubMed:36543799). Ufmylation in response to endoplasmic reticulum stress is essential for processes such as hematopoiesis, blood vessel morphogenesis or inflammatory response (PubMed:32050156). Mediates ufmylation of DDRGK1 and CDK5RAP3; the role of these modifications is however unclear: as both DDRGK1 and CDK5RAP3 act as substrate adapters for ufmylation, it is uncertain whether ufmylation of these proteins is, a collateral effect or is required for ufmylation (PubMed:20018847, PubMed:20531390). Acts as a negative regulator of T-cell activation by mediating ufmylation and stabilization of PDCD1/PD-1 (PubMed:38377992). Also involved in the response to DNA damage: recruited to double-strand break sites following DNA damage and mediates monoufmylation of histone H4 and ufmylation of MRE11 (PubMed:30783677, PubMed:30886146). Mediates ufmylation of TP53/p53, promoting its stability (PubMed:32807901). Catalyzes ufmylation of TRIP4, thereby playing a role in nuclear receptor-mediated transcription (PubMed:25219498). Required for hematopoietic stem cell function and hematopoiesis (By similarity).
Alternative names
KIAA0776, MAXER, NLBP, RCAD, UFL1, E3 UFM1-protein ligase 1, E3 UFM1-protein transferase 1, Multiple alpha-helix protein located at ER, Novel LZAP-binding protein, Regulator of C53/LZAP and DDRGK1
Recommended products
Rabbit Polyclonal UFL1 antibody. C-terminal. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human UFL1 aa 700 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human UFL1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O94874
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab226216 was affinity purified using an epitope specific to UFL1 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
UFL1 also known as E3 ubiquitin-protein ligase UFL1 is a protein involved in the post-translational modification process called ufmylation. This protein has a molecular mass of approximately 34 kDa and facilitates the attachment of the ubiquitin-fold modifier 1 (UFM1) to target proteins. UFL1 is widely expressed in various tissues including the liver heart and kidney. It forms part of a complex that includes UFM1 UBA5 and UFC1 which are all essential for its function.
- Biological function summary
UFL1 regulates proteins' stability and activity by ufmylation in the endoplasmic reticulum (ER) stress response. This protein modifies select substrates enhancing their role in maintaining cellular homeostasis under stress conditions. UFL1 works closely with UFM1 and is part of a complex that targets specific proteins indicating its role in maintaining proper cellular conditions during ER stress. This modification mechanism is important for cells to adapt to changing environments and sustain normal function.
- Pathways
UFL1 plays a role in the ER stress response and protein folding pathways. It operates alongside other proteins including UFM1 which is critical in these processes. UFL1 modulates pathways linked with cellular responses to stress and survival. It interacts with proteins like CDK5 suggesting a role in cellular processes that require precise regulation during stress conditions. Such interactions further integrate UFL1 into overarching cellular survival strategies.
- Associated diseases and disorders
UFL1 has implications in cancer and neurodegenerative diseases. Dysregulation of ufmylation where UFL1 is an important player shows links to tumor progression and survival mechanisms in cancer. UFL1 is also connected to pathological protein aggregation relevant in neurodegenerative diseases. Its interaction with proteins involved in ER stress response suggests a relationship to these conditions. Understanding UFL1's function in these diseases is important for identifying therapeutic targets.
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Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-UFL1 antibody - C-terminal (ab226216)
All lanes: Western blot - Anti-UFL1 antibody - C-terminal (ab226216) at 0.04 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 5: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 90 kDa
Exposure time: 30s
Immunoprecipitation - Anti-UFL1 antibody - C-terminal (ab226216)
UFL1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226216 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab226216 at 0.4 μg/ml.
Lane 1: ab226216 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds
All lanes: Immunoprecipitation - Anti-UFL1 antibody - C-terminal (ab226216)
Predicted band size: 90 kDa
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