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Rabbit Recombinant Monoclonal UFSP2 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 5 publications.

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Images

Western blot - Anti-UFSP2 antibody [EPR13424] (AB185965), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-UFSP2 antibody [EPR13424] (AB185965), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFSP2 antibody [EPR13424] (AB185965), expandable thumbnail
  • Immunoprecipitation - Anti-UFSP2 antibody [EPR13424] (AB185965), expandable thumbnail
  • Western blot - Anti-UFSP2 antibody [EPR13424] (AB185965), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFIHC-P
Human
Tested
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/40 - 1/60
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Thiol-dependent isopeptidase that specifically cleaves UFM1, a ubiquitin-like modifier protein, from conjugated proteins, such as CD274/PD-L1, CYB5R3, DDRGK1, MRE11, RPL26/uL24, TRIP4 and RPL26/uL24 (PubMed:25219498, PubMed:27351204, PubMed:27926783, PubMed:30783677, PubMed:31595041, PubMed:32160526, PubMed:33473208, PubMed:35394863, PubMed:35926457, PubMed:36543799, PubMed:36893266, PubMed:37795761, PubMed:38383785). While it is also able to mediate the processing of UFM1 precursors, a prerequisite for conjugation reactions, UFSP2 mainly acts as a protein deUFMylase that mediates deconjugation of UFM1 from target proteins (PubMed:27926783). Mediates deUFMylation of RPL26/uL24, a critical step to release the UFM1 ribosome E3 ligase (UREL) complex during the recycling of 60S ribosome subunits from the endoplasmic reticulum (PubMed:38383785). Catalyzes deUFMylation of TRIP4, regulating intracellular nuclear receptors transactivation and thereby regulate cell proliferation and differentiation (PubMed:25219498).

Alternative names

C4orf20, UFSP2, Ufm1-specific protease 2, UfSP2

Recommended products

Rabbit Recombinant Monoclonal UFSP2 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 5 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR13424
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

UFSP2 also known as Ubiquitin-Fold Modifier 1-Specific Peptidase 2 is a protein with a molecular mass of approximately 53 kDa. This protein functions as a cysteine protease involved in the processing and maturation of ubiquitin-fold modifier 1 (UFM1). UFSP2 cleaves precursor UFM1 to expose the glycine residue necessary for its conjugation to target proteins. Expression of UFSP2 occurs in various tissues but is particularly high in the testis brain and skeletal muscle indicating its roles in diverse biological functions.

Biological function summary

UFSP2 plays a significant role in the UFMylation pathway which is a post-translational modification process involving UFM1. This enzyme does not operate alone; it acts as part of a functional complex where its peptidase activity is essential for recycling UFM1 molecules after they detach from substrate proteins. The activation and deactivation of UFM1-modified proteins regulate several cellular processes such as protein translation cellular stress responses and maintenance of endoplasmic reticulum homeostasis.

Pathways

The UFSP2 protein contributes to the wider ubiquitin-like modification systems particularly impacting the protein quality control system and the endoplasmic reticulum-associated degradation (ERAD) pathway. It maintains protein folding and ensures degradation of misfolded proteins. UFSP2 interacts with other proteins such as UFL1 which acts as a specific E3 ligase for UFM1 establishing a cycle for efficient modification and de-modification in these pathways.

Associated diseases and disorders

Alterations in UFSP2 function link to pathologies including autosomal-dominant osteosclerosis with the novel symptom of cranial sclerosis (ADOCS). Mutations in the UFSP2 gene can lead to defective bone formation due to dysregulation of protein homeostasis pathways. Furthermore anomalies in UFSP2 have associations with neurodegenerative diseases where it interacts with proteins involved in cellular stress responses. This highlights its importance in both skeletal and nervous system-related disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-UFSP2 antibody [EPR13424] (ab185965), expandable thumbnail

    Western blot - Anti-UFSP2 antibody [EPR13424] (ab185965)

    Lanes 1-3: Merged signal (red and green). Green - ab185965 observed at 53 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    ab185965 Anti-UFSP2 antibody [EPR13424] was shown to specifically react with UFSP2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human UFSP2 knockout HEK-293T cell line ab266368 (knockout cell lysate Human UFSP2 knockout HEK-293T cell lysate ab257782) was used. Wild-type and UFSP2 knockout samples were subjected to SDS-PAGE. ab185965 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-UFSP2 antibody [EPR13424] (ab185965) at 1/1000 dilution

    Lane 1: Wild-type HEK293T cell lysate at 20 µg

    Lane 2: UFSP2 knockout HEK293T cell lysate at 20 µg

    Lane 2: Western blot - Human UFSP2 knockout HEK-293T cell line (Human UFSP2 knockout HEK-293T cell line ab266368)

    Lane 3: HeLa cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Predicted band size: 53 kDa

    Observed band size: 53 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-UFSP2 antibody [EPR13424] (ab185965), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-UFSP2 antibody [EPR13424] (ab185965)

    Immunofluorescent analysis of HeLa cells (-20℃ acetone-fixed) labeling UFSP2 with ab185965 at 1/100 dilution follwed by Goat anti rabbit IgG (AlexaFluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFSP2 antibody [EPR13424] (ab185965), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFSP2 antibody [EPR13424] (ab185965)

    Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling UFSP2 with ab185965 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-UFSP2 antibody [EPR13424] (ab185965), expandable thumbnail

    Immunoprecipitation - Anti-UFSP2 antibody [EPR13424] (ab185965)

    Western blot analysis of immunoprecipitation pellet from Human fetal kidney lysate immunoprecipitated using ab185965 at 1/50 dilution (lane 1) or PBS control (lane 2).
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

    All lanes: Immunoprecipitation - Anti-UFSP2 antibody [EPR13424] (ab185965)

    Predicted band size: 53 kDa

  • Western blot - Anti-UFSP2 antibody [EPR13424] (ab185965), expandable thumbnail

    Western blot - Anti-UFSP2 antibody [EPR13424] (ab185965)

    All lanes: Western blot - Anti-UFSP2 antibody [EPR13424] (ab185965) at 1/10000 dilution

    Lane 1: Human fetal kidney lysate at 20 µg

    Lane 2: Human fetal heart lysate at 20 µg

    Lane 3: Human pancreas lysate at 20 µg

    Lane 4: HeLa cell lysate at 20 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 53 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFSP2 antibody [EPR13424] (ab185965), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFSP2 antibody [EPR13424] (ab185965)

    Immunohistochemical analysis of paraffin-embedded Human kidney clear cell carcinoma tissue labeling UFSP2 with ab185965 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

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Product protocols

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