Anti-UGDH antibody

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGDH antibody (AB155005)

Immunohistochemical analysis of paraffin embedded Human hepatoma tissue labelling UGDH with ab155005 at a 1/500 dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-UGDH antibody (AB155005)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling UGDH protein using ab155005 at a 1/500 dilution (Green).

• WB

Supplier Data

Western blot - Anti-UGDH antibody (AB155005)

Samples were separated by 10% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-UGDH antibody (ab155005) at 1/10000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate at 30 µg

Lane 3:

HeLa whole cell lysate at 30 µg

Lane 4:

HepG2 whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 55 kDa

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• WB

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Western blot - Anti-UGDH antibody (AB155005)

7.5 % SDS-PAGE

All lanes:

Western blot - Anti-UGDH antibody (ab155005) at 1/2000 dilution

All lanes:

rat liver lysate/extract at 50 µg

Predicted band size: 55 kDa

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• WB

Unknown

Western blot - Anti-UGDH antibody (AB155005)

7.5% SDS PAGE

All lanes:

Western blot - Anti-UGDH antibody (ab155005) at 1/10000 dilution

All lanes:

mouse liver whole cell lysate at 50 µg

Predicted band size: 55 kDa

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• WB

CiteAb

Western blot - Anti-UGDH antibody (AB155005)

UGDH western blot using anti-UGDH antibody ab155005. Publication image and figure legend from Saito, T., Ichimura, Y., et al., 2016, Nat Commun, PubMed 27345495.

ab155005 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab155005 please see the product overview.

Persistent activation of Nrf2 in autophagy-deficient mouse livers.(a) Immunoblot analysis. Liver homogenates and nuclear fractions of female Atg7f/f, Atg7f/f;Alb-Cre, Atg7f/f;Nrf2f/f and Atg7f/f;Nrf2f/f;Alb-Cre mice aged at 5 weeks were prepared, and were subjected to immunoblot with the indicated antibodies. (b) Immunofluorescence analysis. Liver sections described in a were double-immunostained with a combination of anti-phosphorylated p62 (green) and anti-p62 (red) antibodies, or of anti-Keap1 (green) and anti-p62 (red) antibodies. Scale bars, 20 μm. (c) Nrf2-dependent gene expressions in autophagy-deficient livers. Total RNAs were prepared from livers of female Atg7f/f (n=4), Atg7f/f;Alb-Cre (n=4), Atg7f/f;Nrf2f/f (n=4) and Atg7f/f;Nrf2f/f;Alb-Cre mice (n=4) aged at 5 weeks. Values were normalized to the amount of mRNA in the livers of Atg7f/f or Atg7f/f;Nrf2f/f mice. The experiments were performed three times. Data are means±s.e. *p<0.05, **p<0.01 and ***p<0.001 as determined by the Welch t-test.

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