AB226172

Anti-UGDH antibody

Name: Anti-UGDH antibody (ab226172) | Abcam
Brand: Abcam Limited
SKU: AB226172

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(1 Publication)

Rabbit Polyclonal UGDH antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human UDP-glucose 6-dehydrogenase aa 1-100.

View Alternative Names

UDP-glucose 6-dehydrogenase, UDP-Glc dehydrogenase, UDP-GlcDH, UDPGDH, UGDH

2 Images

Supplier Data

Immunoprecipitation - Anti-UGDH antibody (AB226172)

UGDH was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226172 at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab226172 at 0.4 μg/ml.

Lane 1 : ab226172 IP in HEK-293T whole cell lysate.

Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-UGDH antibody (ab226172)

Predicted band size: 34 kDa,51 kDa,55 kDa,56 kDa

false

Supplier Data

Western blot - Anti-UGDH antibody (AB226172)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-UGDH antibody (ab226172) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 55 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human UDP-glucose 6-dehydrogenase aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

O60701

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Giant Panda": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab226172 was affinity purified using an epitope specific to UGDH immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UDP-glucose 6-dehydrogenase also known as UGDH is an enzyme with a mass of approximately 53 kDa. It catalyzes the oxidation of UDP-glucose to UDP-glucuronic acid in humans. This enzyme plays an important mechanical role in the biosynthesis of glycosaminoglycans and is expressed in various tissues such as the liver kidneys and lungs. Alternate names for UGDH include UDP-GlcDH and UDPGDH frequently used in scientific literature.

Biological function summary

UDP-glucose 6-dehydrogenase contributes to the conversion processes for nucleotide sugar substrates and it is not reported to be part of a larger enzyme complex. It ensures the availability of UDP-glucuronic acid a necessary precursor for glycosaminoglycans which are components of the extracellular matrix and involved in cellular signaling. This activity supports processes like wound healing and tissue regeneration by maintaining structural integrity and facilitating cell-cell communication in various physiological contexts.

Pathways

UDP-glucose 6-dehydrogenase participates in the hexosamine biosynthetic pathway and the UDP-glucuronate pathway. In the hexosamine biosynthetic pathway it works alongside proteins like glutamine-fructose-6-phosphate transaminase to regulate the production of UDP-GlcNAc important for carbohydrate modifications of proteins and lipids. The UDP-glucuronate pathway involves related enzymes such as hyaluronan synthase which utilizes UDP-glucuronic acid generated by UGDH for synthesizing hyaluronan an important glycosaminoglycan in tissue hydration and lubrication.

Abnormal activity or expression of UDP-glucose 6-dehydrogenase links to cancer and fibrosis. Its role in producing glycosaminoglycans makes it significant in the tumor microenvironment and alterations in UGDH activity may support cancer progression by affecting cell adhesion and migration. In fibrosis overactivity of UGDH can lead to excessive glycosaminoglycan production contributing to tissue scarring. Interactions with proteins like hyaluronan synthase hint at potential therapeutic targets for modifying these extracellular matrix components in disease contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the formation of UDP-alpha-D-glucuronate, a constituent of complex glycosaminoglycans (PubMed : 21502315, PubMed : 21961565, PubMed : 22123821, PubMed : 23106432, PubMed : 25478983, PubMed : 27966912, PubMed : 30420606, PubMed : 30457329). Required for the biosynthesis of chondroitin sulfate and heparan sulfate. Required for embryonic development via its role in the biosynthesis of glycosaminoglycans (By similarity). Required for proper brain and neuronal development (PubMed : 32001716).

See full target information UDP-glucose 6-dehydrogenase

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cellular & molecular immunology 21:770-786 PubMed38839914

2024

HIF-1α and MIF enhance neutrophil-driven type 3 immunity and chondrogenesis in a murine spondyloarthritis model.

Applications

Unspecified application

Species

Unspecified reactive species

Akihiro Nakamura,Sungsin Jo,Sayaka Nakamura,Mansi K Aparnathi,Shaghayegh Foroozan Boroojeni,Mariia Korshko,Ye-Soo Park,Himanshi Gupta,Sandra Vijayan,Jason S Rockel,Mohit Kapoor,Igor Jurisica,Tae-Hwan Kim,Nigil Haroon

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-UGDH antibody

Immunoprecipitation - Anti-UGDH antibody (AB226172)

Western blot - Anti-UGDH antibody (AB226172)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

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Proteins & Peptides

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise