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AB246999

Anti-UGDH antibody

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(1 Publication)

Rabbit Polyclonal UGDH antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human UDP-glucose 6-dehydrogenase aa 400 to C-terminus.

View Alternative Names

UDP-glucose 6-dehydrogenase, UDP-Glc dehydrogenase, UDP-GlcDH, UDPGDH, UGDH

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGDH antibody (AB246999)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGDH antibody (AB246999)

Formalin-fixed, paraffin-embedded human rectum tissue stained for UGDH with ab246999 at a 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGDH antibody (AB246999)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGDH antibody (AB246999)

Formalin-fixed, paraffin-embedded human cerebral cortex tissue shows absence of immunoreactivity for UGDH when incubated with ab246999 at a 1/500 dilution (negative control).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGDH antibody (AB246999)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGDH antibody (AB246999)

Formalin-fixed, paraffin-embedded human liver tissue stained for UGDH with ab246999 at a 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-UGDH antibody (AB246999)
  • WB

Unknown

Western blot - Anti-UGDH antibody (AB246999)

Corresponding UGDH RNA-seq data are presented for the same cell lines. Loading control : Anti-HSP90B1.

All lanes:

Western blot - Anti-UGDH antibody (ab246999) at 0.4 µg/mL

Lane 1:

A549 (human lung carcinoma cell line) whole cell lysate

Lane 2:

HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate

Predicted band size: 55 kDa

false

Western blot - Anti-UGDH antibody (AB246999)
  • WB

Unknown

Western blot - Anti-UGDH antibody (AB246999)

All lanes:

Western blot - Anti-UGDH antibody (ab246999) at 0.4 µg/mL

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

Lane 2:

NBT-II (rat Wistar bladder tumor cell line) whole cell lysate

Predicted band size: 55 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGDH antibody (AB246999)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGDH antibody (AB246999)

Formalin-fixed, paraffin-embedded human prostate tissue stained for UGDH with ab246999 at a 1/500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-UGDH antibody (AB246999)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-UGDH antibody (AB246999)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for UGDH (green) using ab246999 at 4 μg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human UDP-glucose 6-dehydrogenase aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O60701

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UDP-glucose 6-dehydrogenase also known as UGDH is an enzyme with a mass of approximately 53 kDa. It catalyzes the oxidation of UDP-glucose to UDP-glucuronic acid in humans. This enzyme plays an important mechanical role in the biosynthesis of glycosaminoglycans and is expressed in various tissues such as the liver kidneys and lungs. Alternate names for UGDH include UDP-GlcDH and UDPGDH frequently used in scientific literature.
Biological function summary

UDP-glucose 6-dehydrogenase contributes to the conversion processes for nucleotide sugar substrates and it is not reported to be part of a larger enzyme complex. It ensures the availability of UDP-glucuronic acid a necessary precursor for glycosaminoglycans which are components of the extracellular matrix and involved in cellular signaling. This activity supports processes like wound healing and tissue regeneration by maintaining structural integrity and facilitating cell-cell communication in various physiological contexts.

Pathways

UDP-glucose 6-dehydrogenase participates in the hexosamine biosynthetic pathway and the UDP-glucuronate pathway. In the hexosamine biosynthetic pathway it works alongside proteins like glutamine-fructose-6-phosphate transaminase to regulate the production of UDP-GlcNAc important for carbohydrate modifications of proteins and lipids. The UDP-glucuronate pathway involves related enzymes such as hyaluronan synthase which utilizes UDP-glucuronic acid generated by UGDH for synthesizing hyaluronan an important glycosaminoglycan in tissue hydration and lubrication.

Abnormal activity or expression of UDP-glucose 6-dehydrogenase links to cancer and fibrosis. Its role in producing glycosaminoglycans makes it significant in the tumor microenvironment and alterations in UGDH activity may support cancer progression by affecting cell adhesion and migration. In fibrosis overactivity of UGDH can lead to excessive glycosaminoglycan production contributing to tissue scarring. Interactions with proteins like hyaluronan synthase hint at potential therapeutic targets for modifying these extracellular matrix components in disease contexts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the formation of UDP-alpha-D-glucuronate, a constituent of complex glycosaminoglycans (PubMed : 21502315, PubMed : 21961565, PubMed : 22123821, PubMed : 23106432, PubMed : 25478983, PubMed : 27966912, PubMed : 30420606, PubMed : 30457329). Required for the biosynthesis of chondroitin sulfate and heparan sulfate. Required for embryonic development via its role in the biosynthesis of glycosaminoglycans (By similarity). Required for proper brain and neuronal development (PubMed : 32001716).
See full target information UDP-glucose 6-dehydrogenase
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

FEBS open bio 12:1857-1874 PubMed36052535

2022

A cohesin-associated gene score may predict immune checkpoint blockade in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Cui-Zhen Liu,Jian-Di Li,Gang Chen,Rong-Quan He,Rui Lin,Zhi-Guang Huang,Jian-Jun Li,Xiu-Fang Du,Xiao-Ping Lv
View all publications
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