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AB241357

Anti-UGGT/UGT1 antibody

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(1 Publication)

Rabbit Polyclonal UGGT/UGT1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human UGGT1 aa 1500 to C-terminus.

View Alternative Names

GT, UGCGL1, UGGT, UGT1, UGTR, UGGT1, UDP-glucose:glycoprotein glucosyltransferase 1, hUGT1, UDP--Glc:glycoprotein glucosyltransferase, UDP-glucose ceramide glucosyltransferase-like 1

2 Images
Immunoprecipitation - Anti-UGGT/UGT1 antibody (AB241357)
  • IP

Supplier Data

Immunoprecipitation - Anti-UGGT/UGT1 antibody (AB241357)

UGGT/UGT1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241357 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab241357 at 1 μg/ml.

Lane 1 : ab241357 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-UGGT/UGT1 antibody (ab241357)

Predicted band size: 177 kDa

false

Western blot - Anti-UGGT/UGT1 antibody (AB241357)
  • WB

Supplier Data

Western blot - Anti-UGGT/UGT1 antibody (AB241357)

Lysates prepared in NETN buffer.

All lanes:

Western blot - Anti-UGGT/UGT1 antibody (ab241357) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 15 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 15 µg

Predicted band size: 177 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human UGGT1 aa 1500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NYU2

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Alternative Products

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antibody was affinity purified using an epitope specific to UGGT/UGT1 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UGGT also known as UGT1 is a protein that catalyzes the addition of glucose to misfolded glycoproteins in the endoplasmic reticulum (ER). This enzyme weighs approximately 170 kDa and is found mainly in the ER. It functions by recognizing improperly folded proteins and facilitating their retention in the ER through tagging with a glucose moiety for quality control. The targeting of these proteins ensures only properly folded proteins proceed to the Golgi apparatus.
Biological function summary

UGGT/UGT1 plays an important role in the cellular quality control system. It works as part of the calnexin cycle interacting with chaperones like calnexin and calreticulin which recognize the glycosylated proteins. This cycle ensures that proteins fold correctly before they leave the ER. If proteins remain misfolded UGGT/UGT1 continues to add the glucose tag preventing their exit from the ER until correct folding occurs.

Pathways

UGGT/UGT1 is important for the proper functioning of the ER-associated degradation (ERAD) pathway and the unfolded protein response (UPR). In the ERAD pathway UGGT collaborates with proteins such as EDEM and mannosidase I recognizing and degrading misfolded glycoproteins. By doing so UGGT/UGT1 helps maintain cellular homeostasis balancing protein synthesis and degradation within the ER environment to prevent stress and potential damage.

UGGT/UGT1 has connections to conditions like cystic fibrosis and certain congenital disorders of glycosylation. Cystic fibrosis involves improper protein folding where mutations lead to defective protein cycling and mismanagement of chloride channels like CFTR. In cases of congenital disorders of glycosylation UGGT/UGT1 interacts with proteins involved in glycosylation processes leading to altered glycoprotein profiles with varied clinical outcomes. Understanding UGGT/UGT1's interactions can aid in developing therapeutic strategies targeting protein misfolding.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Recognizes glycoproteins with minor folding defects. Reglucosylates single N-glycans near the misfolded part of the protein, thus providing quality control for protein folding in the endoplasmic reticulum. Reglucosylated proteins are recognized by calreticulin for recycling to the endoplasmic reticulum and refolding or degradation.
See full target information UGGT1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 7:392 PubMed34907164

2021

CRISPR/Cas9 genome-wide screening identifies LUC7L2 that promotes radioresistance via autophagy in nasopharyngeal carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Shen,Chao Li,Fang Chen,Liangfang Shen,Zhanzhan Li,Na Li
View all publications
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Product promise

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