AB124879

Anti-UGGT/UGT1 antibody [EPR5105]

RabMAb
Recombinant
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(5 Publications )

Rabbit Recombinant Monoclonal UGGT/UGT1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

View Alternative Names

GT, UGCGL1, UGGT, UGT1, UGTR, UGGT1, UDP-glucose:glycoprotein glucosyltransferase 1, hUGT1, UDP--Glc:glycoprotein glucosyltransferase, UDP-glucose ceramide glucosyltransferase-like 1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGGT/UGT1 antibody [EPR5105] (AB124879)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGGT/UGT1 antibody [EPR5105] (AB124879)

Staining of UGGT/UGT1 in Paraffin-embedded Human brain tissue by Immunohistochemistry using ab124879 at a dilution of 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-UGGT/UGT1 antibody [EPR5105] (AB124879)

All lanes:

Western blot - Anti-UGGT/UGT1 antibody [EPR5105] (ab124879) at 1/10000 dilution

Lane 1:

Human skeletal muscle lysate at 10 µg

Lane 2:

BXPC-3 cell lysate at 10 µg

Lane 3:

SH-SY5Y cell lysate at 10 µg

Lane 4:

MCF7 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 177 kDa

false

Carrier free

Anti-UGGT/UGT1 antibody [EPR5105] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5105

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UGGT also known as UGT1 is a protein that catalyzes the addition of glucose to misfolded glycoproteins in the endoplasmic reticulum (ER). This enzyme weighs approximately 170 kDa and is found mainly in the ER. It functions by recognizing improperly folded proteins and facilitating their retention in the ER through tagging with a glucose moiety for quality control. The targeting of these proteins ensures only properly folded proteins proceed to the Golgi apparatus.

Biological function summary

UGGT/UGT1 plays an important role in the cellular quality control system. It works as part of the calnexin cycle interacting with chaperones like calnexin and calreticulin which recognize the glycosylated proteins. This cycle ensures that proteins fold correctly before they leave the ER. If proteins remain misfolded UGGT/UGT1 continues to add the glucose tag preventing their exit from the ER until correct folding occurs.

Pathways

UGGT/UGT1 is important for the proper functioning of the ER-associated degradation (ERAD) pathway and the unfolded protein response (UPR). In the ERAD pathway UGGT collaborates with proteins such as EDEM and mannosidase I recognizing and degrading misfolded glycoproteins. By doing so UGGT/UGT1 helps maintain cellular homeostasis balancing protein synthesis and degradation within the ER environment to prevent stress and potential damage.

UGGT/UGT1 has connections to conditions like cystic fibrosis and certain congenital disorders of glycosylation. Cystic fibrosis involves improper protein folding where mutations lead to defective protein cycling and mismanagement of chloride channels like CFTR. In cases of congenital disorders of glycosylation UGGT/UGT1 interacts with proteins involved in glycosylation processes leading to altered glycoprotein profiles with varied clinical outcomes. Understanding UGGT/UGT1's interactions can aid in developing therapeutic strategies targeting protein misfolding.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Recognizes glycoproteins with minor folding defects. Reglucosylates single N-glycans near the misfolded part of the protein, thus providing quality control for protein folding in the endoplasmic reticulum. Reglucosylated proteins are recognized by calreticulin for recycling to the endoplasmic reticulum and refolding or degradation.

See full target information UGGT1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Bio-protocol 15:e5206 PubMed39959296

2025

Cell-Sonar, an Easy and Low-cost Method to Track a Target Protein by Expression Changes of Specific Protein Markers.

Applications

Unspecified application

Species

Unspecified reactive species

Sabrina Brockmöller,Lara Maria Molitor,Franz Worek,Simone Rothmiller

eLife 7: PubMed30484775

2018

TAPBPR mediates peptide dissociation from MHC class I using a leucine lever.

Applications

Unspecified application

Species

Unspecified reactive species

F Tudor Ilca,Andreas Neerincx,Clemens Hermann,Ana Marcu,Stefan Stevanović,Janet E Deane,Louise H Boyle

Proceedings of the National Academy of Sciences of 115:E9353-E9361 PubMed30213851

2018

Utilizing TAPBPR to promote exogenous peptide loading onto cell surface MHC I molecules.

Applications

Unspecified application

Species

Unspecified reactive species

F Tudor Ilca,Andreas Neerincx,Mark R Wills,Maike de la Roche,Louise H Boyle

Molecular immunology 113:58-66 PubMed30077416

2018

Preferential interaction of MHC class I with TAPBPR in the absence of glycosylation.

Applications

Unspecified application

Species

Unspecified reactive species

Andreas Neerincx,Louise H Boyle

eLife 6: PubMed28425917

2017

TAPBPR bridges UDP-glucose:glycoprotein glucosyltransferase 1 onto MHC class I to provide quality control in the antigen presentation pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Andreas Neerincx,Clemens Hermann,Robin Antrobus,Andy van Hateren,Huan Cao,Nico Trautwein,Stefan Stevanović,Tim Elliott,Janet E Deane,Louise H Boyle

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-UGGT/UGT1 antibody [EPR5105]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGGT/UGT1 antibody [EPR5105] (AB124879)

Western blot - Anti-UGGT/UGT1 antibody [EPR5105] (AB124879)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise