AB157473

Anti-UGPase antibody [EPR10626]

RabMAb
Recombinant
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(3 Publications)

Rabbit Recombinant Monoclonal UGPase antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

View Alternative Names

UGP1, UGP2, UTP--glucose-1-phosphate uridylyltransferase, UDP-glucose pyrophosphorylase, UDPGP, UGPase

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10626] (AB157473)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10626] (AB157473)

Immunohistochemical analysis of paraffin-embedded Human muscle tissue labeling UGPase with ab157473 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10626] (AB157473)

ab157473 showing +ve staining in Human heart.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-UGPase antibody [EPR10626] (AB157473)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-UGPase antibody [EPR10626] (AB157473)

Intracellular flow cytometric analysis of permeabilized HeLa cells labeling UGPase with ab157473 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

Immunocytochemistry/ Immunofluorescence - Anti-UGPase antibody [EPR10626] (AB157473)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-UGPase antibody [EPR10626] (AB157473)

Immunofluorescent analysis of HeLa cells labeling UGPase with ab157473 at 1/250 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10626] (AB157473)

ab157473 showing +ve staining in Human normal liver.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10626] (AB157473)

ab157473 showing +ve staining in Human normal kidney.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-UGPase antibody [EPR10626] (AB157473)

All lanes:

Western blot - Anti-UGPase antibody [EPR10626] (ab157473) at 1/1000 dilution

Lane 1:

Fetal liver lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 57 kDa

false

Carrier free

Anti-UGPase antibody [EPR10626] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10626

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UGPase also known as UDP-glucose pyrophosphorylase plays an important role in carbohydrate metabolism by catalyzing the reversible formation of UDP-glucose and pyrophosphate from glucose-1-phosphate and UTP. The enzyme has a mass of about 52 kDa. UGPase is expressed in many tissues but especially in liver muscle and brain where carbohydrate metabolism is highly active. Its presence ensures the availability of UDP-glucose which serves as a glycosyl donor in the synthesis of glycogen.

Biological function summary

UGPase provides UDP-glucose a central metabolite in carbohydrate biosynthesis. It does not function as part of a complex but independently supports various biosynthetic pathways. UDP-glucose acts as a precursor not only for glycogen but also for glycoproteins glycolipids and proteoglycans. UGPase activity is critical for maintaining glucose homeostasis and its regulation directly affects carbohydrate allocation and storage.

Pathways

UGPase operates in the glycogen synthesis pathway and the pentose phosphate pathway. In the glycogen synthesis pathway UGPase works closely with glycogen synthase which converts UDP-glucose into glycogen helping in energy storage. In the pentose phosphate pathway UDP-glucose serves as a substrate for enzymes modifying carbohydrate structures. These pathway interactions place UGPase as an essential node linking carbohydrate metabolism routes.

The dysregulation of UGPase affects disorders like glycogen storage diseases and diabetes. An abnormal UGPase activity can lead to inefficient glycogen storage impacting energy balance and contributing to these conditions. The enzyme also connects with proteins like glycogen synthase in glycogen storage diseases illustrating how alterations in its function can disturb glycogen synthesis and storage. Understanding UGPase activity therefore offers insights into metabolic imbalances seen in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

UTP--glucose-1-phosphate uridylyltransferase catalyzing the conversion of glucose-1-phosphate into UDP-glucose, a crucial precursor for the production of glycogen.

See full target information UGP2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The Plant journal : for cell and molecular biology 120:123-138 PubMed39145515

2024

The eukaryotic translation initiation factor eIF4E regulates flowering and circadian rhythm in Arabidopsis.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zhang,Huanhuan Jin,Yadi Chen,Yonghong Jiang,Lianfeng Gu,Guifang Lin,Chentao Lin,Qin Wang

Nature microbiology 8:1252-1266 PubMed37349587

2023

Modelling viral encephalitis caused by herpes simplex virus 1 infection in cerebral organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Agnieszka Rybak-Wolf,Emanuel Wyler,Tancredi Massimo Pentimalli,Ivano Legnini,Anna Oliveras Martinez,Petar Glažar,Anna Loewa,Seung Joon Kim,Benedikt B Kaufer,Andrew Woehler,Markus Landthaler,Nikolaus Rajewsky

Genes & development 28:479-90 PubMed24589777

2014

D-2-hydroxyglutarate produced by mutant IDH2 causes cardiomyopathy and neurodegeneration in mice.

Applications

Species

Unspecified reactive species

Esra A Akbay,Javid Moslehi,Camilla L Christensen,Supriya Saha,Jeremy H Tchaicha,Shakti H Ramkissoon,Kelly M Stewart,Julian Carretero,Eiki Kikuchi,Haikuo Zhang,Travis J Cohoon,Stuart Murray,Wei Liu,Kazumasa Uno,Sudeshna Fisch,Kristen Jones,Sushma Gurumurthy,Camelia Gliser,Sung Choe,Marie Keenan,Jaekyoung Son,Illana Stanley,Julie A Losman,Robert Padera,Roderick T Bronson,John M Asara,Omar Abdel-Wahab,Philip C Amrein,Amir T Fathi,Nika N Danial,Alec C Kimmelman,Andrew L Kung,Keith L Ligon,Katharine E Yen,William G Kaelin,Nabeel Bardeesy,Kwok-Kin Wong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-UGPase antibody [EPR10626]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10626] (AB157473)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10626] (AB157473)

Flow Cytometry (Intracellular) - Anti-UGPase antibody [EPR10626] (AB157473)

Immunocytochemistry/ Immunofluorescence - Anti-UGPase antibody [EPR10626] (AB157473)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10626] (AB157473)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10626] (AB157473)

Western blot - Anti-UGPase antibody [EPR10626] (AB157473)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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