Name: Anti-UGPase antibody [EPR10627(B)]
SKU: ab154817
Rating: 5 (1 reviews)

Rabbit Recombinant Monoclonal UGPase antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

Images

Western blot - Anti-UGPase antibody [EPR10627(B)] (AB154817), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	IP	WB	ICC/IF	Flow Cyt (Intra)
Human	Tested	Not recommended	Tested	Not recommended	Expected
Mouse	Expected	Not recommended	Expected	Not recommended	Tested
Rat	Predicted	Not recommended	Expected	Not recommended	Predicted
Pig	Predicted	Not recommended	Predicted	Not recommended	Predicted

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50 - 1/100	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Rat, Pig	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Human, Rat, Pig	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000 - 1/10000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Pig	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Human, Rat, Pig	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/10 - 1/100	Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Rat, Pig	Dilution info -	Notes -

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

See full target information UGP2

Function

UTP--glucose-1-phosphate uridylyltransferase catalyzing the conversion of glucose-1-phosphate into UDP-glucose, a crucial precursor for the production of glycogen.

Alternative names

UGP1, UGP2, UTP--glucose-1-phosphate uridylyltransferase, UDP-glucose pyrophosphorylase, UDPGP, UGPase

Recommended products

Rabbit Recombinant Monoclonal UGPase antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR10627(B)
Purity: Tissue culture supernatant
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

UGPase also known as UDP-glucose pyrophosphorylase plays an important role in carbohydrate metabolism by catalyzing the reversible formation of UDP-glucose and pyrophosphate from glucose-1-phosphate and UTP. The enzyme has a mass of about 52 kDa. UGPase is expressed in many tissues but especially in liver muscle and brain where carbohydrate metabolism is highly active. Its presence ensures the availability of UDP-glucose which serves as a glycosyl donor in the synthesis of glycogen.

Biological function summary

UGPase provides UDP-glucose a central metabolite in carbohydrate biosynthesis. It does not function as part of a complex but independently supports various biosynthetic pathways. UDP-glucose acts as a precursor not only for glycogen but also for glycoproteins glycolipids and proteoglycans. UGPase activity is critical for maintaining glucose homeostasis and its regulation directly affects carbohydrate allocation and storage.

Pathways

UGPase operates in the glycogen synthesis pathway and the pentose phosphate pathway. In the glycogen synthesis pathway UGPase works closely with glycogen synthase which converts UDP-glucose into glycogen helping in energy storage. In the pentose phosphate pathway UDP-glucose serves as a substrate for enzymes modifying carbohydrate structures. These pathway interactions place UGPase as an essential node linking carbohydrate metabolism routes.

Associated diseases and disorders

The dysregulation of UGPase affects disorders like glycogen storage diseases and diabetes. An abnormal UGPase activity can lead to inefficient glycogen storage impacting energy balance and contributing to these conditions. The enzyme also connects with proteins like glycogen synthase in glycogen storage diseases illustrating how alterations in its function can disturb glycogen synthesis and storage. Understanding UGPase activity therefore offers insights into metabolic imbalances seen in these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Western blot - Anti-UGPase antibody [EPR10627(B)] (ab154817)
All lanes: Western blot - Anti-UGPase antibody [EPR10627(B)] (ab154817) at 1/1000 dilution
Lane 1: HepG2 cell lysate at 10 µg
Lane 2: HeLa cell lysate at 10 µg
Lane 3: NIH 3T3 cell lysate at 10 µg
Lane 4: Human fetal liver tissue lysate at 10 µg
Predicted band size: 57 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] (ab154817)
Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma labeling UGPase using ab154817 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] (ab154817)
Immunohistochemical analysis of paraffin-embedded Human kidney labeling UGPase using ab154817 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry (Intracellular) - Anti-UGPase antibody [EPR10627(B)] (ab154817)
Intracellular flow cytometric analysis of permeabilized NIH 3T3 cells labeling UGPase using ab154817 (red) at 1/10 dilution or a rabbit IgG (negative) (green).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] (ab154817)
Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using ab154817 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] (ab154817)
Immunohistochemical analysis of paraffin embedded Human normal liver tissue using ab154817 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] (ab154817)
Immunohistochemical analysis of paraffin embedded Human normal heart tissue using ab154817 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] (ab154817)
Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab154817 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This image is courtesy of a customer review submitted by Shannon Cruzen
Western blot - Anti-UGPase antibody [EPR10627(B)] (ab154817)
Lanes 1 - 2: Western blot - Anti-UGPase antibody [EPR10627(B)] (ab154817) at 1/10000 dilution
Lanes 3 - 4: Western blot - Anti-UGPase antibody [EPR10627(B)] (ab154817) at 1/20000 dilution
Lanes 5 - 6: Western blot - Anti-UGPase antibody [EPR10627(B)] (ab154817)
All lanes: Pig muscle sarcoplasm tissue lysate at 40 µg
Secondary
All lanes: HRP-conjugated goat anti-rabbit IgG (H+L) polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 45-47 kDa
Exposure time: 5min
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-UGPase antibody [EPR10627(B)] (ab154817)
UGPase western blot using anti-UGPase antibody [EPR10627(B)] ab154817. Publication image and figure legend from Sherman, E. J., Mirabelli, C., et al., 2022, PLoS Pathog, PubMed 35231079.

ab154817 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab154817 please see the product overview.
Interrogation of ACE2 modifiers in Calu-3 cells.(A) FACS plots of ACE2 surface staining in Calu-3 cells treated with control nontargeting (NT) or SMAD4-targeting gRNA for each of 3 technical replicates. (B) Volcano plots of MAGeCK Robust Rank Aggregation scores and gene-level gRNA log2 fold-change in ACE2negative relative to ACE2positive populations for each gene tested in the focused CRISPR library in Calu-3 cells. Genes with FDR <0.05 and absolute log2 fold-change >1 are highlighted, with positive regulators in blue and negative regulators in red. (C) Comparison of log2 fold-change in ACE2negative relative to ACE2positive populations in focused CRISPR screens of HuH7 (x-axis) and Calu-3 (y-axis). Only ACE2, highlighted in blue, was identified in both screens with FDR<0.05 and absolute log2 fold-change >1. Genes identified as significant in only the HuH7 or Calu-3 screens are highlighted in magenta and red, respectively. (D) Immunoblot of lysates collected from Calu-3 cells targeted by CRISPR with a gRNA against the indicated gene or a nontargeting (NT) control sequence. (E) Percentage of cells with detectable surface ACE2 staining after CRISPR-mediated single gene disruption of the indicated target gene or a nontargeting control in Calu-3 cells. Candidate genes associated with positive or negative regulation of ACE2 in the CRISPR screen are highlighted in blue and red, respectively. ACE2-positive gates were defined on control unstained cells and the proportion of ACE2-positive cells for each population is displayed for each of 3–6 technical replicates. Error bars depict standard deviation. Asterisks indicate p<0.05 by Student’s t-test. Source data and statistical analysis are provided in S9 Table.