Rabbit Recombinant Monoclonal UGPase antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse samples.
Images
![Western blot - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (AB249123), expandable thumbnail](https://content.abcam.com/products/images/ugpase-antibody-epr10627b-bsa-and-azide-free-ab249123--western-blot-img103882.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (AB249123)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (AB249123), expandable thumbnail](https://content.abcam.com/products/images/ugpase-antibody-epr10627b-bsa-and-azide-free-ab249123--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129859.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (AB249123)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (AB249123), expandable thumbnail](https://content.abcam.com/products/images/ugpase-antibody-epr10627b-bsa-and-azide-free-ab249123--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img67237.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (AB249123)")
![Flow Cytometry (Intracellular) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (AB249123), expandable thumbnail](https://content.abcam.com/products/images/ugpase-antibody-epr10627b-bsa-and-azide-free-ab249123--flow-cytometry-intracellular-img27476.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (AB249123)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (AB249123), expandable thumbnail](https://content.abcam.com/products/images/ugpase-antibody-epr10627b-bsa-and-azide-free-ab249123--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115282.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (AB249123)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Not recommended | Expected |
| Mouse | Expected | Not recommended | Expected | Not recommended | Tested |
| Rat | Predicted | Not recommended | Predicted | Not recommended | Predicted |
| Pig | Predicted | Not recommended | Predicted | Not recommended | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Pig | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat, Pig | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Pig | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat, Pig | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Pig | Dilution info - | Notes - |
Target data
Function
UTP--glucose-1-phosphate uridylyltransferase catalyzing the conversion of glucose-1-phosphate into UDP-glucose, a crucial precursor for the production of glycogen.
Alternative names
UGP1, UGP2, UTP--glucose-1-phosphate uridylyltransferase, UDP-glucose pyrophosphorylase, UDPGP, UGPase
Recommended products
Rabbit Recombinant Monoclonal UGPase antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR10627(B)
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249123 is the carrier-free version of Anti-UGPase antibody [EPR10627(B)] ab154817.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
UGPase also known as UDP-glucose pyrophosphorylase plays an important role in carbohydrate metabolism by catalyzing the reversible formation of UDP-glucose and pyrophosphate from glucose-1-phosphate and UTP. The enzyme has a mass of about 52 kDa. UGPase is expressed in many tissues but especially in liver muscle and brain where carbohydrate metabolism is highly active. Its presence ensures the availability of UDP-glucose which serves as a glycosyl donor in the synthesis of glycogen.
- Biological function summary
UGPase provides UDP-glucose a central metabolite in carbohydrate biosynthesis. It does not function as part of a complex but independently supports various biosynthetic pathways. UDP-glucose acts as a precursor not only for glycogen but also for glycoproteins glycolipids and proteoglycans. UGPase activity is critical for maintaining glucose homeostasis and its regulation directly affects carbohydrate allocation and storage.
- Pathways
UGPase operates in the glycogen synthesis pathway and the pentose phosphate pathway. In the glycogen synthesis pathway UGPase works closely with glycogen synthase which converts UDP-glucose into glycogen helping in energy storage. In the pentose phosphate pathway UDP-glucose serves as a substrate for enzymes modifying carbohydrate structures. These pathway interactions place UGPase as an essential node linking carbohydrate metabolism routes.
- Associated diseases and disorders
The dysregulation of UGPase affects disorders like glycogen storage diseases and diabetes. An abnormal UGPase activity can lead to inefficient glycogen storage impacting energy balance and contributing to these conditions. The enzyme also connects with proteins like glycogen synthase in glycogen storage diseases illustrating how alterations in its function can disturb glycogen synthesis and storage. Understanding UGPase activity therefore offers insights into metabolic imbalances seen in these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Western blot - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123), expandable thumbnail](https://content.abcam.com/products/images/ugpase-antibody-epr10627b-bsa-and-azide-free-ab249123--western-blot-img103882.jpg "Western blot - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123)")
Western blot - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123)
This data was developed using Anti-UGPase antibody [EPR10627(B)] ab154817, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-UGPase antibody [EPR10627(B)] (Anti-UGPase antibody [EPR10627(B)] ab154817) at 1/1000 dilution
Lane 1: HepG2 cell lysate at 10 µg
Lane 2: HeLa cell lysate at 10 µg
Lane 3: NIH 3T3 cell lysate at 10 µg
Lane 4: Human fetal liver tissue lysate at 10 µg
Predicted band size: 57 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123), expandable thumbnail](https://content.abcam.com/products/images/ugpase-antibody-epr10627b-bsa-and-azide-free-ab249123--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129859.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123)
This data was developed using Anti-UGPase antibody [EPR10627(B)] ab154817, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma labeling UGPase using Anti-UGPase antibody [EPR10627(B)] ab154817 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123), expandable thumbnail](https://content.abcam.com/products/images/ugpase-antibody-epr10627b-bsa-and-azide-free-ab249123--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img67237.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123)
This data was developed using Anti-UGPase antibody [EPR10627(B)] ab154817, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney labeling UGPase using Anti-UGPase antibody [EPR10627(B)] ab154817 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123), expandable thumbnail](https://content.abcam.com/products/images/ugpase-antibody-epr10627b-bsa-and-azide-free-ab249123--flow-cytometry-intracellular-img27476.jpg "Flow Cytometry (Intracellular) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123)")
Flow Cytometry (Intracellular) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123)
This data was developed using Anti-UGPase antibody [EPR10627(B)] ab154817, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of permeabilized NIH 3T3 cells labeling UGPase using Anti-UGPase antibody [EPR10627(B)] ab154817 (red) at 1/10 dilution or a rabbit IgG (negative) (green).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123), expandable thumbnail](https://content.abcam.com/products/images/ugpase-antibody-epr10627b-bsa-and-azide-free-ab249123--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115282.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123)
This data was developed using Anti-UGPase antibody [EPR10627(B)] ab154817, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using Anti-UGPase antibody [EPR10627(B)] ab154817 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123), expandable thumbnail](https://content.abcam.com/products/images/ugpase-antibody-epr10627b-bsa-and-azide-free-ab249123--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132337.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123)
This data was developed using Anti-UGPase antibody [EPR10627(B)] ab154817, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human normal liver tissue using Anti-UGPase antibody [EPR10627(B)] ab154817 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123), expandable thumbnail](https://content.abcam.com/products/images/ugpase-antibody-epr10627b-bsa-and-azide-free-ab249123--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img99009.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123)
This data was developed using Anti-UGPase antibody [EPR10627(B)] ab154817, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human normal heart tissue using Anti-UGPase antibody [EPR10627(B)] ab154817 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123), expandable thumbnail](https://content.abcam.com/products/images/ugpase-antibody-epr10627b-bsa-and-azide-free-ab249123--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118102.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123)
This data was developed using Anti-UGPase antibody [EPR10627(B)] ab154817, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human normal colon tissue using Anti-UGPase antibody [EPR10627(B)] ab154817 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123), expandable thumbnail](https://content.abcam.com/products/images/ugpase-antibody-epr10627b-bsa-and-azide-free-ab249123--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132337.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123), expandable thumbnail](https://content.abcam.com/products/images/ugpase-antibody-epr10627b-bsa-and-azide-free-ab249123--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img99009.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123), expandable thumbnail](https://content.abcam.com/products/images/ugpase-antibody-epr10627b-bsa-and-azide-free-ab249123--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118102.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123)")
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