AB194697

Anti-UGT1A1 antibody

Name: Anti-UGT1A1 antibody (ab194697) | Abcam
Brand: Abcam Limited
SKU: AB194697
Price: 535 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(14 Publications)

Rabbit Polyclonal UGT1A1 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 14 publications. Immunogen corresponding to Recombinant Fragment Protein within Human UDP-glucuronosyltransferase 1A1 aa 1-200.

View Alternative Names

GNT1, UGT1, UGT1A1, UDP-glucuronosyltransferase 1A1, Bilirubin-specific UDPGT isozyme 1, UDP-glucuronosyltransferase 1-1, UDP-glucuronosyltransferase 1A isoform 1, hUG-BR1, UDPGT 1-1, UGT1*1, UGT1-01, UGT1.1

1 Images

Supplier Data

Western blot - Anti-UGT1A1 antibody (AB194697)

All lanes:

Western blot - Anti-UGT1A1 antibody (ab194697) at 1/500 dilution

Lane 1:

HepG2 cell lysate

Lane 2:

SGC996 cell lysate

Lane 3:

SH-SY5Y cell lysate

Lane 4:

mouse liver lysate

Lane 5:

mouse kidney lysate

Lane 6:

mouse lung lysate

Lane 7:

rat liver lysate

Predicted band size: 60 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human UDP-glucuronosyltransferase 1A1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P22309

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UGT1A1 also known as UDP-glucuronosyltransferase 1A1 is an enzyme critical for conjugating bilirubin and various hormones with glucuronic acid. It has a molecular mass of approximately 54 kDa. The enzyme primarily localizes in the liver but is also found in other tissues such as the intestine and kidney. UGT1A1's role involves transforming lipophilic molecules into more water-soluble forms facilitating their excretion from the body.

Biological function summary

UGT1A1 contributes to the detoxification processes by mediating the conjugation of bilirubin a byproduct of red blood cell breakdown. This enzyme is part of the UGT1 complex which includes several enzymes sharing a similar expression pattern and function. Through its activity UGT1A1 helps maintain homeostasis and prevents the buildup of potentially toxic substances in the organism.

Pathways

UGT1A1 is a significant component of the glucuronidation pathway. This pathway is essential for phase II metabolism in the liver working alongside other proteins like UGT1A6 and UGT1A9. The glucuronidation pathway transforms hydrophobic compounds into hydrophilic derivatives for easier elimination. Additionally UGT1A1 activity affects hormone metabolism particularly in the pathways involving estrogen and thyroid hormones.

Mutations or polymorphisms in UGT1A1 relate to conditions such as Gilbert’s syndrome and Crigler-Najjar syndrome. These diseases involve impaired bilirubin clearance leading to jaundice. Connection with other proteins like albumin highlights the collaborative role in bilirubin transport and clearance. Studying UGT1A1 and its interactions continues to be critical for understanding and treating these metabolic disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Isoform 1. UDP-glucuronosyltransferase (UGT) that catalyzes phase II biotransformation reactions in which lipophilic substrates are conjugated with glucuronic acid to increase the metabolite's water solubility, thereby facilitating excretion into either the urine or bile (PubMed : 12181437, PubMed : 15472229, PubMed : 18004206, PubMed : 18004212, PubMed : 18719240, PubMed : 19830808, PubMed : 23288867). Essential for the elimination and detoxification of drugs, xenobiotics and endogenous compounds (PubMed : 12181437, PubMed : 18004206, PubMed : 18004212). Catalyzes the glucuronidation of endogenous estrogen hormones such as estradiol, estrone and estriol (PubMed : 15472229, PubMed : 18719240, PubMed : 23288867). Involved in the glucuronidation of bilirubin, a degradation product occurring in the normal catabolic pathway that breaks down heme in vertebrates (PubMed : 17187418, PubMed : 18004206, PubMed : 19830808, PubMed : 24525562). Also catalyzes the glucuronidation the isoflavones genistein, daidzein, glycitein, formononetin, biochanin A and prunetin, which are phytoestrogens with anticancer and cardiovascular properties (PubMed : 18052087, PubMed : 19545173). Involved in the glucuronidation of the AGTR1 angiotensin receptor antagonist losartan, a drug which can inhibit the effect of angiotensin II (PubMed : 18674515). Involved in the biotransformation of 7-ethyl-10-hydroxycamptothecin (SN-38), the pharmacologically active metabolite of the anticancer drug irinotecan (PubMed : 12181437, PubMed : 18004212, PubMed : 20610558).. Isoform 2. Lacks UGT glucuronidation activity but acts as a negative regulator of isoform 1.

See full target information UDP-glucuronosyltransferase 1A1

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:28552 PubMed40764617

2025

Temporal changes of hepatic drug-metabolizing enzymes mediated by hepatic ischemia-reperfusion injury and the protective effect of Oridonin.

Applications

Unspecified application

Species

Unspecified reactive species

Tanwei Gu,Fahong Dai,Meiqun Cai,Xi Zhang,Danni Xie,Huanguo Jiang,Lingmin Tian,Liyan Yan,Wenxun Lan,Haitao Lv,Lan Tang,Hong Li,Zhaoguo Liu

Biomedical reports 23:109 PubMed40364906

2025

Effect of tripterine on the pharmacokinetics of cyclosporine A and its mechanism in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jinping Zhou,Rui Zhang,Pengpeng Guo,Peixia Li,Shaojun Shi,Yani Liu

The journal of obstetrics and gynaecology research 48:1591-1606 PubMed35445507

2022

Prenatal glucocorticoid administration enhances bilirubin metabolic capacity and increases Ugt1a and Abcc2 gene expression via glucocorticoid receptor and PXR in rat fetal liver.

Applications

Unspecified application

Species

Unspecified reactive species

Tsukasa Kobayashi,Yuko Takeba,Yuki Ohta,Masanori Ootaki,Keisuke Kida,Minoru Watanabe,Taroh Iiri,Naoki Matsumoto

Frontiers in pharmacology 11:628314 PubMed33628187

2021

Neobavaisoflavone Induces Bilirubin Metabolizing Enzyme UGT1A1 via PPARα and PPARγ.

Applications

Unspecified application

Species

Unspecified reactive species

Ya-Di Zhu,Xiao-Qing Guan,Jing Chen,Sheng Peng,Moshe Finel,Ying-Yuan Zhao,Rui-Min Wang,Hui-Chang Bi,Ming Lei,Dan-Dan Wang,Guang-Bo Ge

Frontiers in oncology 10:1509 PubMed32983995

2020

miR-452 Reverses Abnormal Glycosylation Modification of ERα and Estrogen Resistance in TNBC (Triple-Negative Breast Cancer) Through Targeting UGT1A1.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Li,Yidong Zhou,Feng Mao,Songjie Shen,Bin Zhao,Yali Xu,Yan Lin,Xiaohui Zhang,Xi Cao,Ying Xu,Chang Chen,Jinqian Zhang,Qiang Sun

Hepatic medicine : evidence and research 12:79-92 PubMed32607015

2020

In vitro Differentiation of Human TERT-Transfected Multi-Lineage Progenitor Cells (MLPC) into Immortalized Hepatocyte-Like Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel P Collins,Joel H Hapke,Rajagopal N Aravalli,Clifford J Steer

Particle and fibre toxicology 17:22 PubMed32503629

2020

Caloric restriction attenuates C57BL/6 J mouse lung injury and extra-pulmonary toxicity induced by real ambient particulate matter exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Daochuan Li,Shen Chen,Qiong Li,Liping Chen,Haiyan Zhang,Huiyao Li,Dianke Yu,Rong Zhang,Yujie Niu,Shaoyou Lu,Lizhu Ye,Xiaowen Zeng,Guanghui Dong,Rui Chen,Michael Aschner,Yuxin Zheng,Wen Chen

Journal of pharmaceutical analysis 10:263-270 PubMed32612873

2020

An ultra-sensitive and easy-to-use assay for sensing human UGT1A1 activities in biological systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ya-Di Zhu,Hui-Lin Pang,Qi-Hang Zhou,Zi-Fei Qin,Qiang Jin,Moshe Finel,Yi-Nan Wang,Wei-Wei Qin,Yin Lu,Dan-Dan Wang,Guang-Bo Ge

Frontiers in pharmacology 10:1111 PubMed31611795

2019

Oleanolic Acid and Ursolic Acid Induce UGT1A1 Expression in HepG2 Cells by Activating PXR Rather Than CAR.

Applications

Unspecified application

Species

Unspecified reactive species

Na Yao,Caiwen Zeng,Tao Zhan,Fang He,Mingyi Liu,Fanglan Liu,Hong Zhang,Yuqing Xiong,Chunhua Xia

Molecular medicine reports 18:2599-2612 PubMed30015887

2018

Quercetin‑3‑O‑β‑D‑glucoside decreases the bioavailability of cyclosporin A through regulation of drug metabolizing enzymes, transporters and nuclear receptors in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Tingyu Yang,Yani Liu,Xixi Huang,Rui Zhang,Chunxiao Yang,Jiali Zhou,Yu Zhang,Jing Wan,Shaojun Shi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-UGT1A1 antibody

Western blot - Anti-UGT1A1 antibody (AB194697)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (14)

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