AB170858

Anti-UGT1A1 antibody [EPR9592]

Name: Anti-UGT1A1 antibody [EPR9592] (ab170858) | Abcam
Brand: Abcam Limited
SKU: AB170858
Price: 360 USD
Availability: InStock
Rating: 4 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(16 Publications)

Rabbit Recombinant Monoclonal UGT1A1 antibody. Suitable for WB and reacts with Human samples. Cited in 16 publications.

View Alternative Names

GNT1, UGT1, UGT1A1, UDP-glucuronosyltransferase 1A1, Bilirubin-specific UDPGT isozyme 1, UDP-glucuronosyltransferase 1-1, UDP-glucuronosyltransferase 1A isoform 1, hUG-BR1, UDPGT 1-1, UGT1*1, UGT1-01, UGT1.1

3 Images

Supplier Data

Western blot - Anti-UGT1A1 antibody [EPR9592] (AB170858)

All lanes:

Western blot - Anti-UGT1A1 antibody [EPR9592] (ab170858) at 1/1000 dilution

Lane 1:

Human fetal liver lysate at 10 µg

Lane 2:

LoVo lysate at 10 µg

Predicted band size: 47 kDa,60 kDa

Observed band size: 62 kDa

false

Lab

Western blot - Anti-UGT1A1 antibody [EPR9592] (AB170858)

Blocking buffer : 5% NFDM/TBST

Diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-UGT1A1 antibody [EPR9592] (ab170858) at 1/5000 dilution

All lanes:

LoVo (human colorectal adenocarcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 60 kDa

Observed band size: 60 kDa

false

CiteAb

Western blot - Anti-UGT1A1 antibody [EPR9592] (AB170858)

UGT1A1 western blot using anti-UGT1A1 antibody [EPR9592] ab170858. Publication image and figure legend from Gu, C., Li, P., et al., 2019, J Cell Mol Med, PubMed 30950200.

ab170858 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab170858 please see the product overview.

The effect of insulin on the expression of ALB and UGT1A1. A, Secretion of albumin in cell culture supernatant. B, Enzyme activity of UGT1A1 in hiHeps. C, The protein expression of ALB, UGT1A1 and HNF1A in hiHeps treated with 0, 0.5 and 1.0 mg/L insulin by Western blot. D, Expression of the abovementioned proteins was measured in hiHeps transfected with siHNF1A. E, qRT-PCR was performed to analyse the level of related genes in hiHeps after transfection. All data are presented as means ± SD; n = 3 samples per experimental group. *p < 0.05, **p < 0.01

false

Carrier free

Anti-UGT1A1 antibody [EPR9592] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9592

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.16% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UGT1A1 also known as UDP-glucuronosyltransferase 1A1 is an enzyme critical for conjugating bilirubin and various hormones with glucuronic acid. It has a molecular mass of approximately 54 kDa. The enzyme primarily localizes in the liver but is also found in other tissues such as the intestine and kidney. UGT1A1's role involves transforming lipophilic molecules into more water-soluble forms facilitating their excretion from the body.

Biological function summary

UGT1A1 contributes to the detoxification processes by mediating the conjugation of bilirubin a byproduct of red blood cell breakdown. This enzyme is part of the UGT1 complex which includes several enzymes sharing a similar expression pattern and function. Through its activity UGT1A1 helps maintain homeostasis and prevents the buildup of potentially toxic substances in the organism.

Pathways

UGT1A1 is a significant component of the glucuronidation pathway. This pathway is essential for phase II metabolism in the liver working alongside other proteins like UGT1A6 and UGT1A9. The glucuronidation pathway transforms hydrophobic compounds into hydrophilic derivatives for easier elimination. Additionally UGT1A1 activity affects hormone metabolism particularly in the pathways involving estrogen and thyroid hormones.

Mutations or polymorphisms in UGT1A1 relate to conditions such as Gilbert’s syndrome and Crigler-Najjar syndrome. These diseases involve impaired bilirubin clearance leading to jaundice. Connection with other proteins like albumin highlights the collaborative role in bilirubin transport and clearance. Studying UGT1A1 and its interactions continues to be critical for understanding and treating these metabolic disorders.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Isoform 1. UDP-glucuronosyltransferase (UGT) that catalyzes phase II biotransformation reactions in which lipophilic substrates are conjugated with glucuronic acid to increase the metabolite's water solubility, thereby facilitating excretion into either the urine or bile (PubMed : 12181437, PubMed : 15472229, PubMed : 18004206, PubMed : 18004212, PubMed : 18719240, PubMed : 19830808, PubMed : 23288867). Essential for the elimination and detoxification of drugs, xenobiotics and endogenous compounds (PubMed : 12181437, PubMed : 18004206, PubMed : 18004212). Catalyzes the glucuronidation of endogenous estrogen hormones such as estradiol, estrone and estriol (PubMed : 15472229, PubMed : 18719240, PubMed : 23288867). Involved in the glucuronidation of bilirubin, a degradation product occurring in the normal catabolic pathway that breaks down heme in vertebrates (PubMed : 17187418, PubMed : 18004206, PubMed : 19830808, PubMed : 24525562). Also catalyzes the glucuronidation the isoflavones genistein, daidzein, glycitein, formononetin, biochanin A and prunetin, which are phytoestrogens with anticancer and cardiovascular properties (PubMed : 18052087, PubMed : 19545173). Involved in the glucuronidation of the AGTR1 angiotensin receptor antagonist losartan, a drug which can inhibit the effect of angiotensin II (PubMed : 18674515). Involved in the biotransformation of 7-ethyl-10-hydroxycamptothecin (SN-38), the pharmacologically active metabolite of the anticancer drug irinotecan (PubMed : 12181437, PubMed : 18004212, PubMed : 20610558).. Isoform 2. Lacks UGT glucuronidation activity but acts as a negative regulator of isoform 1.

See full target information UDP-glucuronosyltransferase 1A1

style

left-column

Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Translational neuroscience 15:20220357 PubMed39434773

2024

Cystatin C alleviates unconjugated bilirubin-induced neurotoxicity by promoting bilirubin clearance from neurocytes via exosomes, dependent on hepatocyte UGT1A1 activity.

Applications

Unspecified application

Species

Unspecified reactive species

Yating Du,Zhenkun Li

Biological & pharmaceutical bulletin 47:1422-1428 PubMed39111864

2024

Refining Hepatocyte Models to Capture the Impact of CYP2D6*10 Utilizing a PITCh System.

Applications

Unspecified application

Species

Unspecified reactive species

Ryosuke Negoro,Ayu Ouchi,Sayaka Deguchi,Kazuo Takayama,Takuya Fujita

The Journal of biological chemistry 300:107340 PubMed38705390

2024

Triclosan administration to humanized UDP-glucuronosyltransferase 1 neonatal mice induces UGT1A1 through a dependence on PPARα and ATF4.

Applications

Unspecified application

Species

Unspecified reactive species

André A Weber,Xiaojing Yang,Elvira Mennillo,Samantha Wong,Sabrina Le,Jia Ying Ashley Teo,Max Chang,Christopher W Benner,Jeffrey Ding,Mohit Jain,Shujuan Chen,Michael Karin,Robert H Tukey

Molecular therapy. Methods & clinical development 29:32-39 PubMed36936447

2023

Lipid nanoparticle-encapsulated mRNA therapy corrects serum total bilirubin level in Crigler-Najjar syndrome mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Jenny A Greig,Joanna K Chorazeczewski,Vivek Chowdhary,Melanie K Smith,Matthew Jennis,James C Tarrant,Elizabeth L Buza,Kimberly Coughlan,Paolo G V Martini,James M Wilson

Frontiers in pharmacology 13:1053610 PubMed36408246

2022

Dysregulated hepatic UDP-glucuronosyltransferases and flavonoids glucuronidation in experimental colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Wanying Zeng,Xiaojing Liu,Yangyang Wu,Yuting Cai,Zhennan Li,Fei Ye,Yuanhong Sun,Feng Li,Huijie Xing,Shuai Wang

iScience 25:105503 PubMed36404924

2022

Biofabrication of synthetic human liver tissue with advanced programmable functions.

Applications

Unspecified application

Species

Unspecified reactive species

Rodrigo M Florentino,Kazutoyo Morita,Nils Haep,Takashi Motomura,Ricardo Diaz-Aragon,Lanuza A P Faccioli,Alexandra Collin de l'Hortet,Zeliha Cetin,Carla Frau,Lawrence Vernetti,Anna-Klara Amler,Alexander Thomas,Tobias Lam,Lutz Kloke,Kazuki Takeishi,D Lansing Taylor,Ira J Fox,Alejandro Soto-Gutierrez

Biological & pharmaceutical bulletin 45:446-451 PubMed35370268

2022

The Effect of Single-Walled Carbon Nanotubes on UDP-Glucuronosyltransferase 1A Activity in Human Liver.

Applications

Unspecified application

Species

Unspecified reactive species

Yuki Asai,Masayuki Nadai,Miki Katoh

Journal of environmental science and health. Part C, Toxicology and carcinogenesis 40:106-118 PubMed35895929

2022

The expression of Phase II drug-metabolizing enzymes in human B-lymphoblastoid TK6 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xilin Li,Yuxi Li,Kylie G Ning,Si Chen,Lei Guo,Jessica A Bonzo,Nan Mei

Scientific reports 11:11107 PubMed34045606

2021

Potential of therapeutic bile acids in the treatment of neonatal Hyperbilirubinemia.

Applications

Unspecified application

Species

Unspecified reactive species

Lori W E van der Schoor,Henkjan J Verkade,Anna Bertolini,Sanne de Wit,Elvira Mennillo,Eva Rettenmeier,André A Weber,Rick Havinga,Petra Valášková,Jana Jašprová,Dicky Struik,Vincent W Bloks,Shujuan Chen,Andrea B Schreuder,Libor Vítek,Robert H Tukey,Johan W Jonker

Drug metabolism and disposition: the biological fa 48:255-263 PubMed31980500

2020

Differential Role of Liver X Receptor (LXR) and LXR in the Regulation of UDP-Glucuronosyltransferase 1A1 in Humanized Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Eva Hansmann,Elvira Mennillo,Emiko Yoda,Mélanie Verreault,Olivier Barbier,Shujuan Chen,Robert H Tukey

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-UGT1A1 antibody [EPR9592]

Western blot - Anti-UGT1A1 antibody [EPR9592] (AB170858)

Western blot - Anti-UGT1A1 antibody [EPR9592] (AB170858)

Western blot - Anti-UGT1A1 antibody [EPR9592] (AB170858)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (16)

Alternative Products

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise