Mouse Polyclonal UGT1A9 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human UDP-glucuronosyltransferase 1A9.
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Publications
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- Frontiers in pharmacology 13:10536102022Dysregulated hepatic UDP-glucuronosyltransferases and flavonoids glucuronidation in experimental colitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWanying Zeng,Xiaojing Liu,Yangyang Wu,Yuting Cai,Zhennan Li,Fei Ye,Yuanhong Sun,Feng Li,Huijie Xing,Shuai WangPubMed 36408246
- Cell transplantation 23:1229-422013Neonatal livers: a source for the isolation of good-performing hepatocytes for cell transplantation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaia Tolosa,Eugenia Pareja-Ibars,M Teresa Donato,Miriam Cortés,Silvia López,Nuria Jiménez,José Mir,José V Castell,M José Gómez-LechónPubMed 23803290
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human UDP-glucuronosyltransferase 1A9. Database link O60656
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 3 µg/mL | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Isoform 1. UDP-glucuronosyltransferase (UGT) that catalyzes phase II biotransformation reactions in which lipophilic substrates are conjugated with glucuronic acid to increase the metabolite's water solubility, thereby facilitating excretion into either the urine or bile (PubMed:12181437, PubMed:15470161, PubMed:15472229, PubMed:18004212, PubMed:18052087, PubMed:18674515, PubMed:19545173). Essential for the elimination and detoxification of drugs, xenobiotics and endogenous compounds (PubMed:12181437, PubMed:18004212). Catalyzes the glucuronidation of endogenous estrogen hormones such as estradiol and estrone (PubMed:15472229). Also catalyzes the glucuronidation of the isoflavones genistein, daidzein, glycitein, formononetin, biochanin A and prunetin, which are phytoestrogens with anticancer and cardiovascular properties (PubMed:18052087, PubMed:19545173). Involved in the glucuronidation of the AGTR1 angiotensin receptor antagonist caderastan, a drug which can inhibit the effect of angiotensin II (PubMed:18674515). Involved in the biotransformation of 7-ethyl-10-hydroxycamptothecin (SN-38), the pharmacologically active metabolite of the anticancer drug irinotecan (PubMed:12181437, PubMed:20610558). Also metabolizes mycophenolate, an immunosuppressive agent (PubMed:15470161, PubMed:18004212). Isoform 2. Lacks UGT glucuronidation activity but acts as a negative regulator of isoform 1.
Alternative names
GNT1, UGT1, UGT1A9, UDP-glucuronosyltransferase 1A9, UDP-glucuronosyltransferase 1-9, UDP-glucuronosyltransferase 1-I, lugP4, UDPGT 1-9, UGT1*9, UGT1-09, UGT1.9, UGT-1I, UGT1I
Recommended products
Mouse Polyclonal UGT1A9 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human UDP-glucuronosyltransferase 1A9.
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human UDP-glucuronosyltransferase 1A9. Database link O60656
- Carrier free
- Yes
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
UGT1A9 also known as UDP-glucuronosyltransferase 1-9 mechanistically catalyzes the glucuronidation of a variety of substrates including pharmaceuticals endogenous compounds and xenobiotics. It belongs to the UGT1A family with an approximate mass of 56 kDa. UGT1A9 is mainly expressed in the liver but it also shows expression in other tissues like the kidney and the small intestine. The enzyme plays an important role in increasing the solubility of drugs aiding their excretion from the body.
- Biological function summary
UGT1A9 is involved in phase II metabolism contributing to the detoxification processes within the liver. It functions by adding glucuronic acid to lipophilic molecules making them more water-soluble. This glucuronidation process is essential for the clearance of bilirubin steroids and various drugs. UGT1A9 exists as a part of a larger enzyme complex that includes other UGT1A family members. Together these enzymes regulate action and metabolism of diverse physiological substances.
- Pathways
The UGT1A9 enzyme contributes significantly to the glucuronidation pathway and assists in the drug metabolism pathway. It shares functional relationships with other UGT1A enzymes like UGT1A1 particularly in the metabolism of bilirubin. In the drug metabolism pathway UGT1A9 interacts with cytochrome P450 enzymes playing an important role in the reduction of drug toxicity and facilitation of drug clearance.
- Associated diseases and disorders
UGT1A9 has been linked to Gilbert's syndrome and drug-induced hepatotoxicity. Mutations or polymorphisms in the UGT1A9 gene can lead to impaired ability of the enzyme to glucuronidate bilirubin potentially contributing to hyperbilirubinemia in Gilbert's syndrome. UGT1A9 also interacts with proteins such as UGT1A1 in the context of drug-induced liver injury affecting the metabolism of certain therapeutic drugs leading to potential toxic buildup.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-UGT1A9 antibody (ab88517)
All lanes: Western blot - Anti-UGT1A9 antibody (ab88517) at 1 µg/mL
All lanes: Human liver tissue lysate at 50 µg
SecondaryAll lanes: Goat anti-mouse IgG at 1/5000 dilution
Predicted band size: 60 kDa
Western blot - Anti-UGT1A9 antibody (ab88517)
All lanes: Western blot - Anti-UGT1A9 antibody (ab88517) at 1 µg/mL
Lane 1: UGT1A9 transfected 293T cell lysate at 25 µg
Lane 2: Untransfected 293T cell lysate at 25 µg
SecondaryAll lanes: Goat anti-mouse IgG at 1/5000 dilution
Predicted band size: 60 kDa
Observed band size: 58 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGT1A9 antibody (ab88517)
Immunoperoxidase staining of Human small intestine tissue using ab88517 at 3 μg/ml
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