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AB96214

Anti-UGT1A9 antibody

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(3 Publications)

Rabbit Polyclonal UGT1A9 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human UDP-glucuronosyltransferase 1A9 aa 250 to C-terminus.

View Alternative Names

GNT1, UGT1, UGT1A9, UDP-glucuronosyltransferase 1A9, UDP-glucuronosyltransferase 1-9, UDP-glucuronosyltransferase 1-I, lugP4, UDPGT 1-9, UGT1*9, UGT1-09, UGT1.9, UGT-1I, UGT1I

1 Images
Western blot - Anti-UGT1A9 antibody (AB96214)
  • WB

Supplier Data

Western blot - Anti-UGT1A9 antibody (AB96214)

Samples were separated by 0.1 SDS-PAGE.

All lanes:

Western blot - Anti-UGT1A9 antibody (ab96214) at 1/2000 dilution

All lanes:

Mouse liver tissue extracts at 50 µg

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human UDP-glucuronosyltransferase 1A9 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O60656

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UGT1A9 also known as UDP-glucuronosyltransferase 1-9 mechanistically catalyzes the glucuronidation of a variety of substrates including pharmaceuticals endogenous compounds and xenobiotics. It belongs to the UGT1A family with an approximate mass of 56 kDa. UGT1A9 is mainly expressed in the liver but it also shows expression in other tissues like the kidney and the small intestine. The enzyme plays an important role in increasing the solubility of drugs aiding their excretion from the body.
Biological function summary

UGT1A9 is involved in phase II metabolism contributing to the detoxification processes within the liver. It functions by adding glucuronic acid to lipophilic molecules making them more water-soluble. This glucuronidation process is essential for the clearance of bilirubin steroids and various drugs. UGT1A9 exists as a part of a larger enzyme complex that includes other UGT1A family members. Together these enzymes regulate action and metabolism of diverse physiological substances.

Pathways

The UGT1A9 enzyme contributes significantly to the glucuronidation pathway and assists in the drug metabolism pathway. It shares functional relationships with other UGT1A enzymes like UGT1A1 particularly in the metabolism of bilirubin. In the drug metabolism pathway UGT1A9 interacts with cytochrome P450 enzymes playing an important role in the reduction of drug toxicity and facilitation of drug clearance.

UGT1A9 has been linked to Gilbert's syndrome and drug-induced hepatotoxicity. Mutations or polymorphisms in the UGT1A9 gene can lead to impaired ability of the enzyme to glucuronidate bilirubin potentially contributing to hyperbilirubinemia in Gilbert's syndrome. UGT1A9 also interacts with proteins such as UGT1A1 in the context of drug-induced liver injury affecting the metabolism of certain therapeutic drugs leading to potential toxic buildup.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Isoform 1. UDP-glucuronosyltransferase (UGT) that catalyzes phase II biotransformation reactions in which lipophilic substrates are conjugated with glucuronic acid to increase the metabolite's water solubility, thereby facilitating excretion into either the urine or bile (PubMed : 12181437, PubMed : 15470161, PubMed : 15472229, PubMed : 18004212, PubMed : 18052087, PubMed : 18674515, PubMed : 19545173, PubMed : 15231852, PubMed : 21422672, PubMed : 38211441). Essential for the elimination and detoxification of drugs, xenobiotics and endogenous compounds (PubMed : 12181437, PubMed : 18004212). Catalyzes the glucuronidation of endogenous estrogen hormones such as estradiol and estrone (PubMed : 15472229). Involved in the glucuronidation of arachidonic acid (AA) and AA-derived eicosanoids including 15-HETE, PGB1 and F2-isoprostanes (8-iso-PGF2alpha and 5-epi-5-F2t-IsoP) (PubMed : 15231852, PubMed : 38211441). Glucuronates the phytochemical ferulic acid efficently at both the phenolic or the carboxylic acid group (PubMed : 21422672). Also catalyzes the glucuronidation of the isoflavones genistein, daidzein, glycitein, formononetin, biochanin A and prunetin, which are phytoestrogens with anticancer and cardiovascular properties (PubMed : 18052087, PubMed : 19545173). Involved in the glucuronidation of the AGTR1 angiotensin receptor antagonist caderastan, a drug which can inhibit the effect of angiotensin II (PubMed : 18674515). Involved in the biotransformation of 7-ethyl-10-hydroxycamptothecin (SN-38), the pharmacologically active metabolite of the anticancer drug irinotecan (PubMed : 12181437, PubMed : 20610558). Also metabolizes mycophenolate, an immunosuppressive agent (PubMed : 15470161, PubMed : 18004212).. Isoform 2. Lacks UGT glucuronidation activity but acts as a negative regulator of isoform 1.
See full target information UDP-glucuronosyltransferase 1A9
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecules (Basel, Switzerland) 25: PubMed31979355

2020

Dapagliflozin Inhibits Cell Adhesion to Collagen I and IV and Increases Ectodomain Proteolytic Cleavage of DDR1 by Increasing ADAM10 Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Junichi Okada,Eijiro Yamada,Tsugumichi Saito,Hideaki Yokoo,Aya Osaki,Yoko Shimoda,Atsushi Ozawa,Yasuyo Nakajima,Jeffrey E Pessin,Shuichi Okada,Masanobu Yamada

Frontiers in pharmacology 10:1111 PubMed31611795

2019

Oleanolic Acid and Ursolic Acid Induce UGT1A1 Expression in HepG2 Cells by Activating PXR Rather Than CAR.

Applications

Unspecified application

Species

Unspecified reactive species

Na Yao,Caiwen Zeng,Tao Zhan,Fang He,Mingyi Liu,Fanglan Liu,Hong Zhang,Yuqing Xiong,Chunhua Xia

Molecular cancer research : MCR 12:1042-54 PubMed24784839

2014

FGFR3 translocations in bladder cancer: differential sensitivity to HSP90 inhibition based on drug metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Jaime Acquaviva,Suqin He,Chaohua Zhang,John-Paul Jimenez,Masazumi Nagai,Jim Sang,Manuel Sequeira,Donald L Smith,Luisa Shin Ogawa,Takayo Inoue,Noriaki Tatsuta,Margaret A Knowles,Richard C Bates,David A Proia
View all publications
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Product promise

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